- Research article
- Open Access
Phylogeography of higher Diptera in glacial and postglacial grasslands in western North America
BMC Ecology volume 19, Article number: 53 (2019)
- The Correction to this article has been published in BMC Ecology 2020 20:38
Pleistocene glaciations have had an important impact on the species distribution and community composition of the North American biota. Species survived these glacial cycles south of the ice sheets and/or in other refugia, such as Beringia. In this study, we assessed, using mitochondrial DNA from three Diptera species, whether flies currently found in Beringian grasslands (1) survived glaciation as disjunct populations in Beringia and in the southern refugium; (2) dispersed northward postglacially from the southern refugium; or (3) arose by a combination of the two. Samples were collected in grasslands in western Canada: Prairies in Alberta and Manitoba; the Peace River region (Alberta); and the southern Yukon Territory. We sequenced two gene regions (658 bp of cytochrome c oxidase subunit I, 510 bp of cytochrome b) from three species of higher Diptera: one with a continuous distribution across grassland regions, and two with disjunct populations between the regions. We used a Bayesian approach to determine population groupings without a priori assumptions and performed analysis of molecular variance (AMOVA) and exact tests of population differentiation (ETPD) to examine their validity. Molecular dating was used to establish divergence times.
Two geographically structured populations were found for all species: a southern Prairie and Peace River population, and a Yukon population. Although AMOVA did not show significant differentiation between populations, ETPD did. Divergence time between Yukon and southern populations predated the Holocene for two species; the species with an ambiguous divergence time had high haplotype diversity, which could suggest survival in a Beringian refugium.
Populations of Diptera in Yukon grasslands could have persisted in steppe habitats in Beringia through Pleistocene glaciations. Current populations in the region appear to be a mix of Beringian relict populations and, to a lesser extent, postglacial dispersal northward from southern prairie grasslands.
Pleistocene glaciations have left their mark on North America, affecting topography and drainage systems, as well as biota. Changes in climate altered species distributions and community compositions, forcing species southward or into other refugia .
Although the ice receded approximately 10,000 years ago, the effects of the last glaciation are still felt: the continent is still rebounding , many species are left in remnants of their former range where a suitable microclimate has persisted  and populations, once disconnected by ice, still bear the genetic signature of former separation [4, 5]. However, these patterns are intricate due to the complicated nature of landscape changes during glacial cycles; Milankovitch cycles  shaped glacial movements during the Pleistocene, creating cycles of glacial states and interglacials, and asynchronous oscillations of regional ice margins .
Most species survived past glaciations south of the ice sheets [7, 8]. However, the large landmass of Beringia remained unglaciated in the northwest, and was a refugium or a series of refugia during the Pleistocene. The body of evidence that supports the Beringia refugium hypothesis spans many fields such as geology, palynology, biogeography, phylogeography and paleontology [9, 10].
The biogeographic patterns of many Beringian taxa have been explored to infer the glacial history of the region (e.g., [4, 5]). However, because some species assemblages from glaciated Beringia have no modern analogue [10,11,12,13], untangling the history of the Beringian biota is complex.
Xeric grasslands in the Yukon Territory and Alaska are rare and distinctive environments. Characterised by prairie sage (Artemisia frigida Willd.), bunch-grasses and forbs, these isolated communities are associated with arid, exposed, south-facing slopes and have a unique insect fauna. Although located mostly in the southern Yukon, there are northern outliers on steep slopes near Old Crow and along the Yukon and Firth Rivers .
To date, paleoecological and distributional data have been used to infer the origin of these grasslands (e.g., [11, 15, 16]). Fossil evidence suggests that these communities are analogues of the late Pleistocene arctic steppe ecosystem [11,12,13, 17]. Regional aridity during the Wisconsinan glaciation would have allowed this xeric steppe-like flora to be widespread [12, 18]. Subsequent climatic changes have reduced this ecosystem to a few arid, exposed areas.
Some plant and insect distributions suggest that some species in these grasslands may have a southern origin [15, 16] when warm, dry conditions during the Holocene Hypsithermal warm period could have allowed northward expansion of grassland biota. Subsequent cooling, and recession of grasslands, would have left disjunct populations in sites with warmer, drier microclimates. This has been suggested for the occurrence of disjunct northwestern grasslands in the Peace River region of Alberta, up to 54° N [19, 20], but has not been formally tested in the context of xeric Yukon grasslands.
Solecki et al.  analysed the community structure of the family Chloropidae (Diptera) in Yukon xeric grasslands, and in two grassland regions farther south (disjunct grasslands in the Peace River region, Alberta, and in the main body of the prairies in southern Alberta to Manitoba), and found that the Yukon assemblages are distinct from those in southern prairies, and suggested that the Yukon assemblages were a mix of species that persisted in Beringia through glaciation and species that dispersed northward postglacially. Some species in that study were present in all grassland regions studied, but even those widely distributed species may retain, at the population level, genetic evidence of isolation into Beringian and southern populations during glaciation. Such widespread species are the focus of this study.
The objective of this study was to (1) obtain molecular data on population structure of flies in western grasslands (including southern prairie grasslands, Peace River grasslands and Yukon grasslands) and (2) evaluate these population patterns in the context of glacial and postglacial history. Species associated with these habitats may exhibit population patterns that mirror the postglacial history of the habitat. The Yukon grasslands have been hypothesized to be primarily composed of species that persisted in the Pleistocene Beringian steppe; or species that dispersed northward postglacially due to expanding prairie grasslands which later became disjunct because of mid-Holocene climate change.
Diptera populations found in all three grassland regions may have (1) survived glaciation as disjunct populations in Beringia and in the southern refugium; (2) dispersed northward postglacially from the southern refugium; or (3) arisen by a combination of the two. In terms of genetic expectations at the population level, each alternative would have different consequences: (1) would show distinct Yukon haplotypes in a network clearly separated from southern haplotypes; (2) would show haplotypes in the Yukon that are also found in the south, or are linked by short branches to clusters of southern haplotypes; (3) would show a combination of distinct clusters of Yukon haplotypes (reflecting survival and diversification in Beringia), as well as other Yukon haplotypes that are linked to clusters of southern haplotypes (reflecting additional dispersal of the populations northward postglacially).
Phylogeographic patterns for single species can be difficult to decipher due to factors such as mutation rates, gene flow within populations or variable genetic diversity through time . Therefore, we selected three species of Diptera to examine congruence in patterns . A major lineage within the order Diptera are brachyceran flies, or higher Diptera, which are stout flies with shortened antennae. This lineage includes acalyptrate flies, a polyphyletic grouping . The study species were small (2–4 mm) acalyptrate flies which likely have limited dispersal abilities, a trait that could enhance genetic signals of past separation . One of the species (Incertella incerta (Becker) (Chloropidae)) has a continuous distribution in multiple habitats, and two (Trixoscelis fumipennis (Melander) (Heleomyzidae) and Meromyza columbi Fedoseeva (Chloropidae)) are disjunct between the southern grasslands and the Yukon. By using species with different distribution patterns, we hoped to characterize the isolation which may have been due to range disjunction to ensure patterns were due to historical rather than landscape factors .
For each species, we obtained 17–21 sequences per gene (cytochrome c oxidase subunit I (COI) and cytochrome b (Cyt b)) per region, except T. fumipennis in the Prairie region (13 COI, 15 Cyt b sequences) (Additional file 1). Because no major differences were found in separate analyses, all results presented are for the concatenated dataset.
Haplotype and nucleotide diversity
Haplotype and nucleotide diversity were calculated for each species. Both I. incerta and T. fumipennis had the same overall number of haplotypes (35), and similar values of haplotype and nucleotide diversity (Table 1). Although they also had the same number of haplotypes in the Yukon, I. incerta had higher nucleotide diversity in the Yukon, and while I. incerta had the same number of haplotypes (14) in both the Prairies and Peace River, there were more T. fumipennis haplotypes in the Peace River region (18 versus 11).
Overall, I. incerta nucleotide diversity decreased from north to south, but haplotype diversity was similar in the two southern regions and decreased in the north. For T. fumipennis, nucleotide and haplotype diversity were highest in the Peace River region and lowest in the Yukon. The overall nucleotide diversity of M. columbi was much lower than that of the other species and nucleotide and haplotype diversity were highest in the north.
Haplotype networks were built using statistical parsimony to characterize population patterns and examine them visually. Only M. columbi had a haplotype shared among all three regions (Fig. 1b). No haplotypes of I. incerta or T. fumipennis were shared between the two southern regions (Prairies + Peace River) and the Yukon.
There were, however, shared haplotypes for each species between the two southern regions: I. incerta had one shared haplotype between the Prairies and Peace River region (Fig. 2b), M. columbi shared three (Fig. 1b) and T. fumipennis, two (Fig. 3b).
The two species with widespread southern geographic components, I. incerta and T. fumipennis, showed similarities in their networks not exhibited by M. columbi. Both had fewer, more common clustered Yukon haplotypes: I. incerta had two clusters, one of which was separated from other haplotypes by at least six base pair differences; and T. fumipennis had one, separated from other haplotypes by at least five base pair differences. A unique feature of the T. fumipennis network was the lone Yukon haplotype in a cluster of southern haplotypes (South 2, Fig. 3b).
In the M. columbi network, all haplotypes were separated by 1–2 base pair differences and there were fewer more common haplotypes. Despite links to haplotypes of other origins and one shared haplotype, the M. columbi Yukon haplotypes formed two clusters.
Population structure and migration
We estimated the number of populations in our dataset without a priori assumptions using a Bayesian approach in the program Geneland . Multiple runs in Geneland were consistent for each species. For M. columbi and T. fumipennis, two geographically structured populations were found: one Peace River + Prairie, and one Yukon. However, although I. incerta individuals grouped into the same geographically structured populations, Geneland recognized three populations. The third “ghost population”, which contained no individuals, was likely an artefact of the Bayesian analysis overestimating the genetic structure due to isolation by distance or data that did not adhere to modelling assumptions . Guillot  recommended ignoring these ghost populations and Frantz et al.  suggested testing for isolation by distance. As expected, the partial Mantel test showed that I. incerta did exhibit isolation by distance, but population structure was also correlated with genetic distance when removing the effect of geographic distance (p = 0.06, R = 0.77), implying that population disjunction also plays a role in forming this pattern. The two other species overall did not show isolation by distance (results not shown).
We used analysis of molecular variance (AMOVA) to test for differentiation among the populations defined by Geneland. The AMOVA showed no significant differentiation between the southern population grouping of the Peace River and Prairie regions, and a Yukon population, for any species (p ~ 0.3, for all ΦCT) (Table 2).
An exact test of population differentiation (ETPD) was performed on this same population structure. The ETPD results were not concordant with AMOVA; there was a significant difference between the southern population assemblage (Peace River + Prairies) and the Yukon population (Table 3). In addition, comparisons among the two southern assemblages were not significant, supporting the population delimitations suggested by Geneland.
Migration was estimated using M values (absolute value of migrants between populations per generation). The M values showed that migration between the Peace River region and Prairies was high for all species, especially T. fumipennis whose M value was estimated to be infinity (Table 3). Although unrealistic, this value stems from an Fst value approaching 0, meaning that there is no differentiation between the two regions. In contrast, migration between those two regions and the Yukon was low for all species.
Estimates of divergence time between populations
Dated Bayesian phylogenies were produced for each species to establish divergence times. Trees recovered groupings found in haplotype networks but did not resolve uncertain relationships. Clusters, particularly those from a single region of origin, tended to be supported in both networks and trees (e.g., Yukon 2 in the I. incerta tree) (Fig. 2). Unresolved regions in the haplotype networks were reflected in the Bayesian analyses through low posterior values (e.g., node of group Yukon 1, Yukon 2 and PRR in M. columbi tree) (Fig. 1).
The method used to find divergence times yielded conservative time intervals for nodes spanning hundreds of thousands and sometimes more than a million years. Furthermore, it is difficult to provide estimates for the divergence of Yukon populations from southern ones. Yukon individuals were not always assigned to the same monophyletic grouping (e.g., Yukon 1, 2 and 3 in M. columbi tree, Fig. 1a). In addition, branches at divergence nodes often had low posterior values (e.g., node of the group containing Yukon 1, Yukon 2, PRR in M. columbi tree, Fig. 1a). Posterior values below 0.7 are not reported. Nevertheless, nodes at the base of groupings (which represent the group’s time to most recent common ancestor (Tmrca, in million years (Myr)) with high posteriors allows placement of the origin of the group within a time frame. Incertalla incerta (Fig. 2a) and T. fumipennis (Fig. 3a) have nodes at the base of Yukon groups with high posteriors which range from well before the beginning of the Wisconsinan glacial, which began approximately 120 kya, and from before or during the Pleistocene glaciations (which began approximately 3 Mya ). The highest posterior density (HPD) interval of expansion for the Yukon population of T. fumipennis was 0.33–1.2 Mya (mean = 0.83) (Fig. 3a). For I. incerta, one of the Yukon groupings (Yukon 1) had an HPD interval between 0.16 and 0.7 Mya (mean = 0.67), and the Yukon 2 grouping 0.21–1.29 Mya (mean = 0.98) (Fig. 2a).
For M. columbi, only one of the nodes of a Yukon grouping had a high posterior (Yukon 3), with an HPD interval between 0 and 0.54 Mya (mean = 0.38) (Fig. 1a).
Survival in Beringia, northward dispersal, or both?
Phylogeographic studies have shown that Beringia has been a refugium for species in multiple taxa, including plants, mammals and fish . Our results mirror, at the population level, the patterns we found at the species level , in that widespread Diptera species in xeric Yukon grasslands are likely a mix of populations that apparently persisted in Beringian steppe communities through the Pleistocene, and those that dispersed northward during the Holocene. There was evidence for population differentiation between the Yukon and southern regions in some analyses (haplotype networks, ETPD, Geneland), although AMOVA results differed.
Trixoscelis fumipennis showed the most distinct division between Yukon and southern populations, with all Yukon haplotypes, except one, in a single group (Fig. 3b) whose divergence dates to the Pleistocene, based on Tmrca (Fig. 3a). This is suggestive of persistence of populations in Beringia during Pleistocene glaciation. The single Yukon haplotype in the South 2 group, otherwise containing only prairie and Peace region haplotypes, suggests a separate dispersal northward postglacially.
Haplotype networks and Bayesian analyses for I. incerta and M. columbi do not show such a clear pattern of population divergence.
In the two Yukon clusters of Incertella incerta (Fig. 2), the time intervals at the base overlap (0.16–0.7 vs. 0.21–1.29). It is possible that they originated at the same time and that additional sampling would recover missing intermediate haplotypes. However, the low haplotype diversity in the Yukon suggests that this is unlikely (Table 1). The number of base pairs separating the Yukon 2 cluster from the others accounts for high nucleotide diversity in this region (Fig. 2b), and may reflect a longer history of divergence, supported by the branch length of the Yukon 2 group (Fig. 2a).
The branches preceding the Yukon 1 group in I. incerta are short and nodes have low posterior values. Although this could be due to conflicting phylogenetic signal, this pattern is seen during rapid diversification events in species trees . A rapid expansion event in an intraspecific tree would likely show the same signal. It is thus likely that this group has a more recent history.
This overall pattern is consistent with survival of populations of I. incerta in Beringia during the Pleistocene. While the region remained unglaciated, multiple glacials and interglacials affected species ranges, creating bottlenecks during glacials and allowing expansion during interglacials. The genetic patterns of I. incerta suggest that it could have been affected by at least two such events. The few frequent haplotypes in each group suggests that both were subjected to bottlenecks, and the branch lengths connected to each group suggest that they were affected by bottleneck events at different times.
Some phylogeographic studies have found population substructure within Beringia , as we did with I. incerta. In the ground squirrel, Spermophilus parryii Richardson (Rodentia: Sciuridae) at least four clades whose divergences can be dated back to glaciation events have persisted through several glacials . The spruce beetle, Dendroctonus rufipennis Kirby (Coleoptera: Curculionidae), also exhibits two distinct clades in Beringia separated by a more southern clade which suggests secondary contact between both northern clades following glacial cycles .
In the case of our study, the precise reason for a population substructure in the I. incerta Yukon groups is unclear. The geographic extent of our sampling was limited and does not allow us to determine whether this substructure could be due to populations residing in different refugia, for instance. Many patterns of Beringian population substructure have been detected in different taxa  and our results warrant future investigation.
The haplotype network and dated tree of M. columbi are also difficult to interpret (Fig. 1). Few nodes have high posteriors and the HPD interval of 0–0.54 at the base of the Yukon 3 group encompasses the Holocene and the late Pleistocene. The low posterior values of the nodes and short branches preceding groups Yukon 1 and Yukon 2 suggest a period of rapid change, but it is difficult to speculate beyond this. Nonetheless, the high haplotype diversity in the Yukon, coupled with few shared haplotypes with the South, suggests survival of populations in Beringia . Haplotype diversity of M. columbi was higher in the Yukon than in the other regions (Table 1), a pattern often associated with survival in a refugium , although this was not the case with the other species. The single widespread haplotype of M. columbi found in all three regions (Fig. 1b) suggests recent dispersal, consistent with a pattern seen in I. incerta.
Although results suggested that populations of all three species survived in Beringia during the Pleistocene, patterns differed considerably between species. This is not surprising given the differences in geographic distributions of each species, and their trophic roles. The lower haplotype and nucleotide diversity for the phytophagous species M. columbi, which has more disjunct populations compared to the other species, is consistent with patterns in butterflies with disjunct versus widespread distributions in the prairies and Peace River regions . In such species, the distribution of suitable host plants is a factor in determining distribution. This may not be the case for generalist saprophagous species such as I. incerta or T. fumipennis. The more obvious genetic split between Yukon and southern populations of T. fumipennis mirrors the apparent disjunction in its overall distribution: it is widespread in southern Canada and the western United States but Foster and Mathis  recorded no specimens between prairie grassland sites in western Canada (British Columbia, Alberta, Saskatchewan) and the southern Yukon.
There are a few possible explanations for the disagreement between AMOVA (no significant population structure) and ETPD (significant difference in population structure). When examining disjunct and continuous butterfly species, Bromilow and Sperling  found that species with continuous distributions tended to lack significant population structure. This would correspond well with the distribution of I. incerta and it could be that the distributions of the other two species have not been recorded adequately. However, this would contradict ETPD results. The non-significant AMOVA could also be an artefact of small sample size. While this may be true particularly for T. fumipennis, where there was lower sampling in the Prairies, the sampling for M. columbi seems to have been sufficient given the higher frequency of many haplotypes. Another possibility is that the non-significance is due to the high variance both among population and within region, particularly for I. incerta and T. fumipennis. Certain haplotypes, even within populations or regions, are highly differentiated with many base pair differences in both the south and the Yukon. The presence of two Yukon groups of I. incerta haplotypes indicates population substructure which, among other explanations, could be due two different glaciation events.
Bromilow and Sperling  evaluated the population structure of continuously distributed and disjunct butterfly species in the Peace River and southern grasslands. Unlike their study, we did not find any significant population structure in the two southern regions. We also found more gene flow between both regions than Bromilow and Sperling  found in butterflies. The M values between the Peace River and Prairies were considerably higher (> 12.5) for all of our species than those found for any of the continuous species in their study (highest value: 10.24; average: 4.71). This was unexpected, because acalyptrate Diptera are thought to be poor fliers. One possible explanation for the gene flow between individuals from the Peace River and Prairies is that because of their small size acalyptrate flies can be passively dispersed over long distances by wind (e.g., [34, 35]). It may also be that populations of the Diptera species remained larger through time, and/or experienced fewer bottlenecks than Lepidoptera, and thus have retained higher genetic polymorphism.
Divergence time estimates are critical in testing phylogeographic hypotheses , but this is not a simple matter in Diptera, particularly Schizophora. Although strict-clock models tend to be appropriate for intraspecific datasets such as ours, the rate of evolution is a required parameter [37, 38]. Rates are known for mitochondrial genes of some Diptera, but individual Schizophora families exhibit substantially different diversification rates . In addition, mutation rates may be time-scale dependent, where recently derived rates (through pedigree and laboratory studies) do not necessarily reflect rates on relevant time scales .
Because of the Pleistocene-Holocene time frame of our study, recent calibration points would have provided more accurate rate estimates for divergence time . However, the massive Tertiary diversification of Schizophora has masked more recent phylogenetic patterns, and existing deep fossil calibration points for flies are not specific to our study taxa . In addition, deep calibration times are problematic beyond time-dependency biases. Divergence in genes often predates population divergence and this can also lead to an overestimate of divergence times.
To mitigate issues regarding time-dependency due to calibration constraints, we used multiple demographic models depending on whether the data was interspecific or intraspecific . Other methods have been suggested to deal with these issues, such as expansion dating where a well-documented population expansion was used to calibrate rates . However, the lack of reliable data on phylogeny, diversity and population patterns in many Diptera limits the possible approaches to obtaining divergence time estimates. Although it is possible that our method has inflated divergence times, our estimates do fit the time frame of the Pleistocene glaciations .
While our analyses are concordant with one another, this study was based on two mitochondrial genes, which represent only the matrilineal side and may not fully reflect population history . Additional mitochondrial or nuclear genes could provide more insight into these patterns.
Our analyses support the conclusion that populations of Diptera in Yukon grasslands could have persisted in steppe habitats in Beringia through Pleistocene glaciations. Current populations in the region appear to be a mix of Beringian relict populations and, to a lesser extent, postglacial dispersal northward from southern prairie grasslands.
Given the limited current extant and potential glacial history of xeric Yukon grasslands, they have been surprisingly understudied. Most research to date has been focused on paleoecological data and current species assemblages, and not on phylogeography or genetic patterns (e.g., [21, 44,45,46]). In a comparison of plant species present in Alaska, in the Boreal forest on the northern Great Plains, and the southwest Yukon, Vetter  found that 25% of plant species in each region were restricted to that region. These grasslands are not uniform in their composition and could potentially have different origins or at least be good systems in which to study modern landscape genetics if these differences are recent. These grasslands offer a unique opportunity to study ice age dynamics through extant systems.
Most phylogeographic work with a Beringian scope has been on arctic and alpine organisms (e.g., most examples in Shafer et al. ). However, xeric Beringian grasslands may be as vulnerable to climate change as other Arctic ecosystems. Conway and Danby  found a reduction in the extent of grassland due to forest encroachment, particularly on flat terrain and south-facing slopes near Kluane Lake, Yukon. Although more restricted than other arctic ecosystems, these grasslands host a unique species assemblage. Some insects, such as the weevil Connatichela artemisiae Anderson (Coleoptera: Curculionidae), are endemic to eastern Beringian grasslands , and some potentially endemic Diptera await formal description (A.M. Solecki and J.J. Mlynarek, unpublished data). Our study has shown that flies present on these south-facing slopes represent unique genetic lineages. The insect fauna of these grasslands may be as distinct and unique as the grasslands themselves.
Diptera were collected from grasslands in three regions in Canada: Prairies (Alberta, 5 sites; Saskatchewan, 1 site; Manitoba, 2 sites), the Peace River region (Alberta, 2 sites), and southern Yukon Territory (4 sites) (Table 4, Fig. 4).
Vegetation in the Prairies is broadly characterized by grasses (Poaceae), sedges (Cyperaceae), Asteraceae, especially sages (Artemisia), and other forbs . Sampling focused on dry sites in the mixed grassland ecoregion, dominated by blue grama, speargrass, low sedge and Artemisia frigida . Sites characterized by different vegetation (e.g., tall-grass prairie, Cypress Uplands) were also sampled for widespread Diptera species.
The Peace River grasslands are isolated from the southern Prairies by 300–400 km and are restricted to the Peace River valley and its tributaries . Sampling in the Peace River was restricted to xeric, steep slopes which tend to have Hesperostipa spartea-Carex-A. frigida associations .
Yukon grasslands are characterized by A. frigida, bunch grasses and forbs and are generally associated with arid, exposed south-facing slopes .
Diptera were collected into 95% ethanol and dried with hexamethyldisilazane prior to mounting. Specimens were identified to species to determine those present in all three regions. Geographic distributions were determined by creating maps  using published literature [33, 52] and museum records [53, 54] (Additional file 2). Three species were selected for analysis: Incertella incerta (Becker) (Chloropidae), a widespread Nearctic generalist saprophagous species, present in habitats between the study regions (Fig. 5); Meromyza columbi Fedoseeva (Chloropidae), a phytophagous western Nearctic grassland species disjunct between the three study regions  (Fig. 6); and Trixoscelis fumipennis (Melander) (Heleomyzidae), an apparently saprophagous species widespread in the southern Nearctic, south of the Peace River region, but disjunct between Peace River and the Yukon (Fig. 7). Although T. fumipennis is mostly present at disturbed sites in its southern range , in the Yukon it has been collected almost exclusively in xeric grassland sites.
DNA extraction, amplification and sequencing protocols follow Gibson et al. . Total genomic DNA was extracted using whole specimens. For each species, 20–21 specimens were extracted for each region. Since specimens were mounted on points, water was used to dissolve glue prior to extraction when necessary. The DNA was extracted using a DNeasy Tissue kit (Qiagen Inc., Santa Clara, CA, USA). After extraction, specimens were critical point dried. All specimens were assigned unique identifiers and vouchers are deposited at the Lyman Entomological Museum, McGill University (Table 5).
Two mitochondrial gene regions were targeted and amplified: (1) a 658 bp fragment of cytochrome c oxidase subunit I (COI) (the DNA barcode) using the forward primer LC01490 (5′-GGTCAACAAATCATAAAGATATTGG-3′)  and the reverse primer COI-Dipt-2183R (5′-CCAAAAAATCARAATARRTGYTG-3′) ; (2) a 510 bp fragment of cytochrome b using the forward primer CytB-Dipt-11035F (5′-GGNTTYKCNGTNGAYAAYGC-3′)  and the reverse primer CytB-Dipt-11545R (5′-ACDGGDCGDGCYCCRATTC-3′) . Amplifications were carried out in 25 μL reactions: 16.75 μL ddH2O, 2.5 μL 10X Ex-Taq PCR buffer (containing 20 mM MgCl2), 0.625 μL 25 mM MgCl2, 1 μL of each 10 μM primer, 2 μL 10 μM dNTPs, 0.125 μL ExTaq HS DNA polymerase (Takara Bio USA, Madison, WI, USA), and 1 μL genomic DNA template. Amplification cycles were performed on an Eppendorf ep Gradient S Mastercycler (Eppendorf AG, Hamburg, Germany) as follows: 94 °C for 3 min; 30 amplification cycles of 94 °C for 45 s, 45 °C for 45 s, 72 °C for 1 min; and a final step for 5 min at 72 °C.
Amplification products were visualised on 1% agarose electrophoresis gels and target genes were isolated and purified using the E-Gel® system (Invitrogen™, Carlsbad, CA, USA) as outlined in Gibson et al. . Purified products were sequenced at the Agriculture & Agri-Food Canada, Eastern Cereal and Oilseed Research Centre Core Sequencing Facility (Ottawa, ON, Canada). The same primers used in PCR reactions were used to sequence forward and reverse strands. Sequencing reactions were carried out in a volume of 10 μL and used an ABI BigDye® Terminator v3.1 Cycle Sequencing kit (PE Applied Biosystems, Foster City, CA, USA).
Chromatograms for sequences LEM0276023–0276140 were edited and visualised using Sequencher 4.7 (Gene Codes Corp., Ann Arbour, MI, USA). Other chromatograms (sequences LEM0049920, LEM0049922–0049923, LEM0276204–0276286) were edited and visualised using ChromasPro (Technelysium, South Brisbane, QLD, Australia).
Sequences were aligned using Clustal X v.2.0 with default parameters . Overhangs were removed in BioEdit v.7.2.3 . Nucleotide sequences were translated into amino acids using the invertebrate mitochondrial genetic code with ORF Finder  to place sequences in the appropriate reading frame. GenBank numbers for all sequences are in Table 5.
All analyses were performed on the concatenated dataset. Mitochondrial haplotype diversity (h) and nucleotide diversity (π) were calculated for the entire dataset and for each region using the program DnaSP v.5 .
Haplotype networks were constructed using statistical parsimony with the program TCS v.1.2.1  with a 95% cut-off value for parsimonious branch connections between haplotypes.
To avoid a priori assumptions about the data, the program Geneland v.4.0.3  run in R  was used to estimate the number of populations in the total sample. It uses a Bayesian approach that can incorporate molecular and geographic data to estimate population clusters without prior population definitions .
The Geneland analysis was performed under the correlated allele frequencies model. Geographic coordinates (WGS84) were converted to UTM. Coordinate uncertainty did not affect results and thus, for the final runs, none was assigned. The analysis was executed for five runs, with 1,000,000 Markov chain Monte Carlo (MCMC) iterations each. Thinning was set at 400. The minimum and maximum value of K (number of populations) was set to 1 and 10, respectively. Burn-in was set to 2000.
Because Bayesian methods can overestimate population structure when there is isolation by distance , we examined correlation between geographic and genetic distance using partial Mantel tests executed in Isolation by distance web service v.3.23 . The partial Mantel test compares genetic and geographic distances while allowing additional variables to be incorporated into the test and for their effects to be isolated . We removed the effect of pre-existing population structure due to disjunction to verify that patterns were not just due to isolation by distance . To build this indicator matrix, for each pairwise comparison, a value of 0 was given when both individual sequences came from the same population and 1 when they did not. For each species, 10,000 randomizations were performed. The genetic distances were calculated using ΦST and Kimura’s Two-Parameter Model which accounts for different rates of transition and transversion .
Population structures defined by Geneland were tested within an AMOVA framework and with ETPD. Both were implemented in Arlequin v.3.5 . The AMOVA was examined at three hierarchical levels: ΦST—within region (regions being defined as Prairies, Peace River region or Yukon), ΦSC—within regions among populations and ΦCT—among populations as defined by Geneland. Arlequin was also used to calculate M values using the formula M = (1 − FST)/2FST .
Divergence times were calculated with BEAST v.1.7.5  and the output examined via Tracer v.1.6 . Because there are no suitable recent calibration points or intraspecific mutation rates for the study taxa, we used fossil data to estimate mutation rates. A dated Diptera tree was calibrated using fossils for specific clades and dates obtained at the nodes of the relevant lineages were used for subsequent analyses. This method was adapted from Nardi et al.  and Marino et al. .
The Diptera tree was built using sequences of Cyclorrhapha, Schizophora and Acalyptratae obtained from GenBank (Additional file 3). The two fossil dating points used were: 70 Mya for Schizophora [24, 73] and 42 Mya for Chloropidae  (Table 6). The Hasegawa-Kishino-Yano (HKY) nucleotide substitution model was chosen for sequence evolution  with partition of nucleotides into their separate coding positions and rate variation described by a four category discrete distribution. Models that consider codons tend to outperform models that do not, even when fewer parameters are considered . The two genes were unlinked to allow separate base frequencies to be estimated. A relaxed lognormal clock model was used to allow different rates of evolution for each branch . The tree prior for this interspecific Diptera tree was set as the Yule process , a model appropriate for multiple species. MCMC chain length was set to 100 million, with a 10% burn-in. Convergence was confirmed by examining Effective Sample Size (ESS > 200). Other parameters were set to the default.
A simplified phylogeny of each study taxon was then generated, using one sequence per haplotype and the other Chloropidae species to root the tree. For each subset, the HKY model with invariant sites was selected based on results from jModelTest 2 (v.2.1.4) . Nucleotides were partitioned into their separate coding positions. Posterior distributions from the Diptera tree were used as priors to calibrate the Chloropidae and the study taxa nodes (Table 6, Additional file 3). The intraspecific aspect of the data was ignored and a Birth–Death prior was used . The analyses were performed under a strict clock for a MCMC chain length of 10 million (10% burn-in). A strict molecular clock was used for this and the following analyses due to the intraspecific nature of the data and the expected low rate of variation between branches . Analyses were verified for convergence (ESS > 200).
Demographic analyses were run using BEAST, for each species separately. All sequences available for the study taxa were used. Tree shape was defined by a Bayesian skyline prior, a variable population size coalescent model . The Tmrca, and mutation rate for each taxon from the simplified phylogenies were used as priors (Table 6). Analyses were run for a MCMC length of 30 million (ESS > 200) with a 10% burn-in. Maximum clade credibility trees were viewed in FigTree v.1.4.0 .
Availability of data and materials
The sequences used in this study have been deposited in GenBank under the accession numbers outlined in Table 5.
analysis of molecular variance
cytochrome c oxidase subunit I
- Cyt b :
effective sample size
exact tests of population differentiation
- h :
highest posterior density
thousand years ago
- M value:
absolute value of migrants between populations per generation
Markov Chain Monte Carlo
million years ago
time to most recent common ancestor (in millions of years)
Hewitt G. The genetic legacy of the Quaternary ice ages. Nature. 2000;405:907–13.
Menzies J. The Pleistocene legacy: glaciation. In: Orme A, editor. The physical geography of North America. New York: Oxford University Press; 2002. p. 36–54.
Kuzmina S, Elias S, Matheus P, Storer JE, Sher A. Paleoenvironmental reconstruction of the Last Glacial Maximum, inferred from insect fossils from a tephra buried soil at Tempest Lake, Seward Peninsula, Alaska. Palaeogeogr Palaeocl. 2008;267:245–55.
Soltis DE, Morris AB, McLachlan JS, Manos PS, Soltis PS. Comparative phylogeography of unglaciated eastern North America. Mol Ecol. 2006;15:4261–93.
Shafer ABA, Cullingham CI, Côté SD, Coltman DW. Of glaciers and refugia: a decade of study sheds new light on the phylogeography of northwestern North America. Mol Ecol. 2010;19:4589–621.
Pielou EC. After the Ice Age: the return of life to glaciated North America. Chicago: The University of Chicago Press; 1991.
Scudder GGE. Present patterns in the fauna and flora of Canada. In: Danks HV, editor. Canada and its insect fauna. Memoirs of the Entomological Society of Canada No. 108. Ottawa: Tyrell Press Limited; 1979. p. 87–179.
Beatty GE, Provan J. Refugial persistence and postglacial recolonization of North America by the cold-tolerant herbaceous plant Orthilia secunda. Mol Ecol. 2010;19:5009–21.
DeChaine EG. A bridge or a barrier? Beringia’s influence on the distribution and diversity of tundra plants. Plant Ecol Divers. 2008;1:197–207.
Elias SA, Crocker B. The Bering Land Bridge: a moisture barrier to the dispersal of steppe-tundra biota? Quat Sci Rev. 2008;27:2473–83.
Schweger CE. Late quaternary palaeoecology of the Yukon: a review. In: Danks HV, Downes JA, editors. Insects of the Yukon. Ottawa: Biological Survey of Canada (Terrestrial Arthropods); 1997. p. 59–72.
Zazula GD, Froese DG, Schweger CE, Mathewes RW, Beaudoin AB, Telka AM, Harington CR, Westgate JA. Ice-age steppe vegetation in east Beringia. Nature. 2003;423:603.
Zazula GD, Schweger CE, Beaudoin AB, McCourt GH. Macrofossil and pollen evidence for full-glacial steppe within an ecological mosaic along the Bluefish River, eastern Beringia. Quatern Int. 2006;142–143:2–19.
Scudder GGE. Environment of the Yukon. In: Danks HV, Downes JA, editors. Insects of the Yukon. Ottawa: Biological Survey of Canada (Terrestrial Arthropods); 1997. p. 13–57.
Finnamore AT. Aculeate wasps (Hymenoptera: Aculeata) of the Yukon, other than Formicidae. In: Danks HV, Downes JA, editors. Insects of the Yukon. Ottawa: Biological Survey of Canada (Terrestrial Arthropods); 1997. p. 867–900.
Boucher S, Wheeler TA. Diversity of Agromyzidae (Diptera) in disjunct grasslands of the southern Yukon Territory. Can Entomol. 2001;133:593–621.
Willerslev E, Davison J, Moora M, Zobel M, Coissac E, Edwards ME, Lorenzen ED, Vestergård M, Gussarova G, Haile J, Craine J, Gielly L, Boessenkool S, Epp LS, Pearman PB, Cheddadi R, Murray D, Bråthen KA, Yoccoz N, Binney H, Cruaud C, Wincker P, Goslar T, Alsos IG, Bellemain E, Brysting AK, Elven R, Sønstebø JH, Murton J, Sher A, Rasmussen M, Rønn R, Mourier T, Cooper A, Austin J, Möller P, Froese D, Zazula G, Pompanon F, Rioux D, Niderkorn V, Tikhonov A, Savvinov G, Roberts RG, MacPhee RDE, Gilbert MTP, Kjær KH, Orlando L, Brochmann C, Taberlet P. Fifty thousand years of Arctic vegetation and megafaunal diet. Nature. 2014;506:47–51.
Guthrie RD. Origin and causes of the mammoth steppe: a story of cloud cover, woolly mammal tooth pits, buckles, and inside-out Beringia. Quat Sci Rev. 2001;20:549–74.
Strong WL, Hills LV. Post-hypsithermal plant disjunctions in western Alberta, Canada. J Biogeogr. 2003;30:419–30.
Strong WL, Hills LV. Late-glacial and Holocene palaeovegetation zonal reconstruction for central and north-central North America. J Biogeogr. 2005;32:1043–62.
Solecki AM, Buddle CM, Wheeler TA. Distribution and community structure of chloropid flies (Diptera: Chloropidae) in Nearctic glacial and post-glacial grasslands. Insect Cons Div. 2016;9:358–68.
Bromilow SM, Sperling FAH. Phylogeographic signal variation in mitochondrial DNA among geographically isolated grassland butterflies. J Biogeogr. 2011;38:299–310.
Riddle BR, Hafner DJ. Phylogeography in historical biogeography: investigating the biogeographic histories of populations, species and young biotas. In: Ebach MC, Tangney RS, editors. Biogeography in a changing world. Boca Raton: CRC Press; 2006. p. 161–76.
Wiegmann BM, Trautwein MD, Winkler IS, Barr NB, Kim J-A, Lambkin C, Bertone MA, Cassel BK, Bayless KM, Heimberg AM, Wheeler BM, Peterson KJ, Pape T, Sinclair BJ, Skevington JH, Blagoderov V, Caravas J, Kutty SN, Schmidt-Ott U, Kampmeier GE, Thompson FC, Grimaldi DA, Beckenbach AT, Courtney GW, Friedrich M, Meier R, Yeates DK. Episodic radiations in the fly tree of life. Proc Natl Acad Sci USA. 2011;108:5690–5.
Federov VB, Stenseth NC. Multiple glacial refugia in the North American Arctic: inference from phylogeography of the collared lemming (Dicrostonyx groenlandicus). Proc R Soc B. 2002;269:2071–7.
Guillot G, Estoup A, Mortier F, Cosson JF. A spatial statistical model for landscape genetics. Genetics. 2005;170:1261–80.
Guillot G. Inference of structure in subdivided populations at low levels of genetic differentiation—the correlated allele frequencies model revisited. Bioinformatics. 2008;24:2222–8.
Frantz AC, Cellina S, Krier A, Schley L, Burke T. Using spatial Bayesian methods to determine the genetic structure of a continuously distributed population: clusters or isolation by distance? J Appl Ecol. 2009;46:493–505.
Lambeck K, Esat TM, Potter E-K. Links between climate and sea levels for the past three million years. Nature. 2002;419:199–206.
He K, Shinohara A, Jiang X-L, Campbell KL. Multilocus phylogeny of talpine moles (Talpini, Talpidae, Eulipotyphla) and its implications for systematics. Mol Phylogenet Evol. 2014;70:513–21.
Eddingsaas AA, Jacobsen BK, Lessa EP, Cook JA. Evolutionary history of the arctic ground squirrel (Spermophilus parryii) in Nearctic Beringia. J Mammal. 2004;85:601–10.
Maroja LS, Bogdanowicz SM, Wallin KF, Raffa KF, Harrison R. Phylogeography of spruce beetles (Dendroctonus rufipennis Kirby) (Curculionidae: Scolytinae) in North America. Mol Ecol. 2007;16:2560–73.
Foster GA, Mathis WN. A revision of the Nearctic species of the genus Trixoscelis Rondani (Diptera: Heleomyzidae: Trixoscelidinae). Smithson Contrib Zool. 2011;637:1–128.
Johnson CG, Taylor LR, Southwood TRE. High altitude migration of Oscinella frit L. (Diptera: Chloropidae). J Anim Ecol. 1962;31:373-383.
Holzapfel EP, Harrell JC. Transoceanic dispersal studies of insects. Pac Insects. 1968;10:115–53.
Wang Q, Mao K-S. Puzzling rocks and complicated clocks: how to optimize molecular dating approaches in historical phytogeography. New Phytol. 2016;209:1353–8.
Brown RP, Yang Z. Rate variation and estimation of divergence times using strict and relaxed clocks. BMC Evol Biol. 2011;11:271.
Ho SYW, Duchêne S. Molecular-clock methods for estimating evolutionary rates and timescales. Mol Ecol. 2014;23:5947–65.
Ho SYW, Lanfear R, Bromham L, Phillips MJ, Sourbier J, Rodrigo AG, Cooper A. Time-dependent rates of molecular evolution. Mol Ecol. 2011;20:3087–101.
Hoareau TB. Late glacial demographic expansion motivates a clock overhaul for population genetics. Syst Biol. 2016;65:449–64.
Marino IAM, Pujolar JM, Zane L. Reconciling deep calibration and demographic history: Bayesian inference of post glacial colonization patterns in Carcinus aestuarii (Nardo, 1847) and C. maenas (Linnaeus, 1758). PLoS ONE. 2011;6:e28567.
Crandall ED, Sbrocco EJ, DeBoer TS, Barber PH, Carpenter KE. Expansion dating: calibrating molecular clocks in marine species from expansions onto the Sunda Shelf following the Last Glacial Maximum. Mol Biol Evol. 2012;29:707–19.
Bazin E, Glémin S, Galtier N. Population size does not influence mitochondrial genetic diversity in animals. Science. 2006;312:570–2.
Boucher S. Diversity and zoogeography of Brachycera (Diptera) in disjunct grasslands of the southern Yukon. M.Sc. Thesis, McGill University, Montreal; 1998.
Vetter MA. Grasslands of the Aishihik-Sekulmun Lakes area, Yukon Territory, Canada. Arctic. 2000;53:165–73.
Berman D, Alfimov A, Kuzmina S. Invertebrates of the relict steppe ecosystems of Beringia, and the reconstruction of Pleistocene landscapes. Quat Sci Rev. 2011;30:2200–19.
Conway AJ, Danby RK. Recent advance of forest-grassland ecotones in southwestern Yukon. Can J For Res. 2014;44:509–20.
Anderson RS. An overview of the beetles (Coleoptera) of the Yukon. In: Danks HV, Downes JA, editors. Insects of the Yukon. Ottawa: Biological Survey of Canada (Terrestrial Arthropods); 1997. p. 405–44.
Shorthouse JD. Ecoregions of Canada’s prairie grasslands. In: Shorthouse JD, Floate KD, editors. Arthropods of Canadian Grasslands (volume 1): ecology and interactions in grassland habitats. Ottawa: Biological Survey of Canada; 2010. p. 53–81.
Schmidt BC, Sperling FAH, Macaulay AD. Moths and butterflies (Lepidoptera) of the Peace River Region: case study of a disjunct grassland fauna. In: Giberson DJ, Cárcamo HA, editors. Arthropods of Canadian Grasslands (volume 4): biodiversity and systematics, part 2. Ottawa: Biological Survey of Canada; 2014. p. 241–67.
Shorthouse DP. SimpleMappr, an online tool to produce publication-quality point maps. http://www.simplemappr.net. Accessed 19 June 2016.
Fedoseeva LI. A revision of the Nearctic species of grass flies of the genus Meromyza Mg. (Diptera, Chloropidae). Entomol Rev. 1971;50:520–9.
Canadensys. http://www.canadensys.net. Accessed 1 Oct 2014.
Ratnasingham S, Hebert PDN. BOLD: The barcode of life data system (www.barcodinglife.org). Mol Ecol Notes. 2007;7:355–64.
Gibson JF, Skevington JH, Kelso S. Placement of Conopidae (Diptera) within Schizophora based on mtDNA and nrDNA gene regions. Mol Phylogenet Evol. 2010;56:91–103.
Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994;3:294–9.
Gibson JF, Kelso S, Jackson MD, Kits JH, Miranda GFG, Skevington JH. Diptera-specific polymerase chain reaction amplification primers of use in molecular phylogenetic research. Ann Entomol Soc Am. 2011;104:976–97.
Gibson JF, Kelso S, Skevington JH. Band-cutting no more: a method for the isolation and purification of target PCR bands from multiplex PCR products using new technology. Mol Phylogenet Evol. 2010;56:1126–8.
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG. Clustal W and Clustal X version 2.0. Bioinformatics. 2007;23:2947–8.
Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser. 1999;41:95–8.
Librado P, Rozas J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009;25:1451–2.
Clement M, Posada D, Crandall KA. TCS: a computer program to estimate gene genealogies. Mol Ecol. 2000;9:1657–9.
Guillot G, Mortier F, Estoup A. Geneland: a computer package for landscape genetics. Mol Ecol Notes. 2005;5:712–5.
R Development Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org/. Accessed 24 Sept 2013.
Jensen JL, Bohonak AJ, Kelley ST. Isolation by distance, web service. BMC Genet. 2005;6:13. http://ibdws.sdsu.edu/. Accessed 1 Aug 2013.
Manel S, Schwartz MK, Luikart G, Taberlet P. Landscape genetics: combining landscape ecology and population genetics. Trends Ecol Evol. 2003;18:189–97.
Ngan EC. Isolation by distance web service with incorporation of DNA datasets. M.Sc. Thesis, San Diego State University, San Diego; 2006.
Excoffier L, Laval G, Schneider S. Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform. 2005;1:47–50.
Slatkin M. Inbreeding coefficients and coalescence times. Genet Res. 1991;58:167–75.
Drummond AJ, Suchard MA, Xie D, Rambaut A. Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol. 2012;29:1969–73.
Rambaut A, Suchard MA, Xie D, Drummond AJ. Tracer v.1.6: MCMC trace analysis package. 2014. http://beast.bio.ed.ac.uk/Tracer. Accessed 7 Apr 2014.
Nardi F, Carapelli A, Boore JL, Roderick GK, Dallai R, Frati F. Domestication of olive fly through a multi-regional host shift to cultivated olives: comparative dating using complete mitochondrial genomes. Mol Phylogenet Evol. 2010;57:678–86.
Hasegawa M, Kishino H, Yano T. Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol. 1985;22:160–74.
Shapiro B, Rambaut A, Drummond AJ. Choosing appropriate substitution models for the phylogenetic analysis of protein-coding sequences. Mol Biol Evol. 2006;23:7–9.
Drummond AJ, Ho SYW, Phillips MJ, Rambaut A. Relaxed phylogenetics and dating with confidence. PLoS Biol. 2006;4:e88.
Gernhard T. The conditioned reconstructed process. J Theor Biol. 2008;253:769–78.
Darriba D, Taboada GL, Doallo R, Posada D. jModelTest 2: more models, new heuristics and parallel computing. Nat Methods. 2012;9:772.
Drummond AJ, Rambaut A, Shapiro B, Pybus OG. Bayesian coalescent inference of past population dynamics from molecular sequences. Mol Biol Evol. 2005;22:1185–92.
Rambaut A. FigTree v.1.4.0: Tree figure drawing tool. 2012. http://tree.bio.ed.ac.uk/software/figtree/. Accessed 5 Jan 2013.
We thank S. Rochefort and E. Vajda for assistance in the field and lab, and J.J. Mlynarek for specimens from the Prairies. Thanks to S. Kelso for help with the molecular work. Collecting permits were granted by the Yukon Government.
This research was supported by: the Natural Sciences and Engineering Research Council of Canada (Canada Graduate Scholarship to AMS (supporting the design of the study, and analysis of specimen data); Strategic Project Grant to CMB, TAW and DC Currie (supporting the design of the study, field work for specimen collection in 2011, and analysis of specimen data); Discovery Grant and Northern Research Supplement to TAW (supporting field work for specimen collection in 2012, acquisition of data and interpretation of data); Discovery Grant to JHS (supporting data collection and student training)); Agriculture and Agri-Food Canada (supporting data collection and student training); the Northern Scientific Training Program (supporting field work for AMS); Fonds de recherche du Québec—Nature et technologies (Masters Research Scholarship) (supporting data analysis and interpretation for AMS); the Canadian Northern Studies Trust; and the W. Garfield Weston Foundation (supporting data analysis, interpretation and manuscript writing for AMS).
Ethics approval and consent to participate
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
The original version of this article was revised: Following publication of the original article, the authors reported an error with the copyright holder.
Coordinates for distribution maps of all 3 species. Each excel sheet contains the coordinates used to create the distribution map of one of the species along with the locality information and source.
GenBank accession numbers for Diptera phylogeny. Additional taxa (with GenBank accession numbers) used for calculating divergence times.
About this article
Cite this article
Solecki, A.M., Skevington, J.H., Buddle, C.M. et al. Phylogeography of higher Diptera in glacial and postglacial grasslands in western North America. BMC Ecol 19, 53 (2019). https://doi.org/10.1186/s12898-019-0266-4
- Cyt b