- Open Access
BioVeL: a virtual laboratory for data analysis and modelling in biodiversity science and ecology
BMC Ecology volume 16, Article number: 49 (2016)
Making forecasts about biodiversity and giving support to policy relies increasingly on large collections of data held electronically, and on substantial computational capability and capacity to analyse, model, simulate and predict using such data. However, the physically distributed nature of data resources and of expertise in advanced analytical tools creates many challenges for the modern scientist. Across the wider biological sciences, presenting such capabilities on the Internet (as “Web services”) and using scientific workflow systems to compose them for particular tasks is a practical way to carry out robust “in silico” science. However, use of this approach in biodiversity science and ecology has thus far been quite limited.
BioVeL is a virtual laboratory for data analysis and modelling in biodiversity science and ecology, freely accessible via the Internet. BioVeL includes functions for accessing and analysing data through curated Web services; for performing complex in silico analysis through exposure of R programs, workflows, and batch processing functions; for on-line collaboration through sharing of workflows and workflow runs; for experiment documentation through reproducibility and repeatability; and for computational support via seamless connections to supporting computing infrastructures. We developed and improved more than 60 Web services with significant potential in many different kinds of data analysis and modelling tasks. We composed reusable workflows using these Web services, also incorporating R programs. Deploying these tools into an easy-to-use and accessible ‘virtual laboratory’, free via the Internet, we applied the workflows in several diverse case studies. We opened the virtual laboratory for public use and through a programme of external engagement we actively encouraged scientists and third party application and tool developers to try out the services and contribute to the activity.
Our work shows we can deliver an operational, scalable and flexible Internet-based virtual laboratory to meet new demands for data processing and analysis in biodiversity science and ecology. In particular, we have successfully integrated existing and popular tools and practices from different scientific disciplines to be used in biodiversity and ecological research.
Environmental scientists, biologists and ecologists are pressed to provide convincing evidence of contemporary changes to biodiversity, to identify factors causing biodiversity decline, to predict the impact of, and suggest ways of combating biodiversity loss. Altered species distributions, the changing nature of ecosystems and increased risks of extinction, many of which arise from anthropogenic activities all have an impact in important areas of societal concern (human health and well-being, food security, ecosystem services, bioeconomy, etc.). Thus, scientists are asked to provide decision support for managing biodiversity and land-use at multiple scales, from genomes to species and ecosystems, to prevent or at least to mitigate such losses. Generating enough evidence and providing decision support increasingly relies on large collections of data held in digital formats, and the application of substantial computational capability and capacity to analyse, model, simulate and predict using such data [1–3]. Achieving the aims of the recently established Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES)  requires progressive developments in approach and method.
The complexity and scope of analyses in biodiversity science and ecology is growing very fast. It is becoming more common to carry out complex analysis using hundreds of data files with different structures and data types (e.g., genetic, species, geographical, environmental) combined with a variety of algorithms; producing results that need to be visualized in innovative ways. The requirement for scientists to work together, with collaborations that integrate datasets across many different parties and synthesize answers computationally to address larger scientific questions are becoming the norm. Biodiversity science and ecology are now in the era of data-intensive science [5, 6]. New research practices that productively exploit data pipelines and data-driven analytics need infrastructure that enables reliability, robustness, repeatability, provenance and reproducibility for large and complex scientific investigations. Methods evolve, exploiting tendencies to base on variants of previous processes, composed of common steps. However, usage statistics from developed science-wide e-Infrastructures show that biodiversity, conservation, and ecology scientists do not carry out large-scale experiments to the same extent as scientists in the physical sciences .
Scientific workflow systems, such as Kepler , Pegasus , Apache Taverna , VisTrails , KNIME , Galaxy  and RapidMiner  are mature technology for practical ways to carry out computer-based experimentation and analysis of relevant data in disciplines as diverse as medical ‘omics’/life sciences, heliophysics and toxicology [15–17]. Scientific workflow systems can be broadly organised into three categories. First, those developed for specialist domains, often with capabilities to be extended to other disciplines (e.g., LONI pipeline for neuro-imaging ; Galaxy for omics data processing; KNIME for pharmacological drug discovery). Secondly, there are workflow systems developed to be independent of any particular science discipline, with features for incorporating specialised customisations (e.g., Apache Taverna). Thirdly, there are those that cut across disciplines and focus on specific tasks (e.g., RapidMiner for data mining).
Workflows support both automation of routine tasks (data transformation, mining, integration, processing, modelling) and pursuit of novel, large-scale analysis across distributed data resources. Today, such activities are typically done in the R environment and here the integration with workflow systems can add value to current practice in ecological research. Most importantly, the record of what was done (the provenance) and the reproducibility of complex analyses can be enhanced when migrating ecological analysis into workflow environments, while workflow systems are able to handle the procedures for scaling computation on cloud infrastructure, for example. For this purpose, scientific workflow systems are starting to become used in biodiversity science and ecology for example: in ecology [19, 20], niche and distribution modelling [21–23], and digitisation of biological collections [24, 25].
Workflows can be deployed, executed, and shared in virtual laboratories. A modern virtual laboratory (sometimes also known as a virtual research environment, VRE) is a web-enabled software application that brings the digital, Internet-based data resources (which may include data collections, databases, sensors and/or other instruments) together with processing and analytical tools needed to carry out “in silico” or “e-science” work. As in a real laboratory, the essence of a virtual laboratory is providing the capability to carry out experimental work as a sequence of interconnected work processes i.e., a workflow. Data and tools are combined harmoniously to present a consistent joined-up computer-based work environment to the scientist user. The laboratory keeps track of the details of experiments designed and executed, as well as creating relevant provenance information about the data and tools used; to assist repeatability and replication of results. A virtual laboratory often also incorporates elements to provide assistance and to support collaborations between persons and across teams. These can include sharing and publishing mechanisms for data, experiments and results, as well as supplemental communications capabilities (either built-in or external) for Web-based audio/video conferencing, email and instant messaging, technical training and support.
The aim of our work has been to explore use of the workflow approach in ecology and to encourage wider adoption and use by developing, deploying and operating this Biodiversity Virtual e-Laboratory as a showcase for what is possible and as an operational service . We have demonstrated this with results from a number of scientific studies that have used the BioVeL platform (Additional file 4).
The biodiversity virtual e-laboratory (BioVeL) provides a flexible general-purpose approach to processing and analysing biodiversity and ecological data (such as species distribution, taxonomic, ecological and genetic data). It is based on distributed computerised services accessible via the Internet that can be combined into sequences of steps (workflows) to perform different tasks.
The main components of the platform are illustrated in Fig. 1 and described following, with cross-references (A)–(F) between the text and the figure. Additional file 1 to the present article provides ‘how-to’ guidelines on how to make use of the various components.
Web services for biodiversity science and ecology: (A) in Fig. 1
In computing terms, Web services are pieces of computing functionality (analytical software tools and data resources) deployed at different locations on the Internet (Worldwide Web) . The idea of presenting data resources and analytical tools as Web services is an essential principle of the notion of the Worldwide Web as a platform for higher value “Software as a Service” applications, meaning users have to install less and less specialised software on their local desktop computers. Web services are central to the concept of workflow composition and execution; increasingly so with proliferation of third-party data resources and analytical tools, and trends towards open data and open science. Wrapping data resources and analytical tools to present the description of their interfaces and capabilities in a standard way aids the process of matching the outputs of one element in a workflow sequence to the inputs of the next. Where such matches are inexact, specialised services can be called upon to perform a translation function. Another benefit of describing resources and functions in a standardised way is the ability to register and advertise details in a catalogue akin to a ‘Yellow Pages’ directory, such that the resources and tools can be more easily discovered by software applications.
Many candidate Web services, representing useful biodiversity data resources and analytical tool capabilities can be identified from the different thematic sub-domains of biodiversity science. These include services coming from domains of enquiry such as: taxonomy, phylogenetics, metagenomics, ecological niche and population modelling, and ecosystem functioning and valuation; as well as more generally useful services relating to statistics, data retrieval and transformations, geospatial processing, and visualization. Working with domain experts via a series of workshops during 2012–2013 and other community networking mechanisms, we considered and prioritised more than 60 candidate services in seven groups (Table 1) many of which went on to be further developed, tested and deployed by their owning “Service Providers”. A full list of services is available in the Additional information.
We have catalogued these capabilities (Web services) in a new, publicly available, curated electronic directory called the Biodiversity Catalogue (http://www.biodiversitycatalogue.org) . This is an openly available online registry of Web services targeted towards the biodiversity science and ecology domain. It is an instance of software developed originally by the BioCatalogue project for the life sciences community , branded and configured for use in ecology. Our intention is that this catalogue should be well-founded through careful curation, and should become well-known and used, as is the case for the BioCatalogue in life sciences. The catalogue uses specialised service categories and tag vocabularies for describing services specific to biodiversity science and ecology and it has been operational since October 2012. Currently (date of writing), it catalogues 70+ services (some of which are aggregates of multiple capabilities) from 50+ service providers, including Global Biodiversity Information Facility (GBIF), European Bioinformatics Institute (EBI), EUBrazilOpenBio, PenSoft Publishers, Royal Botanic Gardens Kew, Species2000/ITIS Catalogue of Life (CoL), Pangaea, World Register of Marines Species (WoRMS), Naturalis Biodiversity Center and Canadensys). It has 130+ contributing members and is open for any provider of similar kinds of capabilities to register their Web services there.
The catalogue supports registration, discovery, curation and monitoring of Web services. Catalogue entries are contributed by the community and also curated by the community. Experts oversee the curation process to ensure that descriptions are high quality and that the services entries are properly annotated. We developed a 4-level service maturity model to measure the quality of service descriptions and annotations. Biodiversity Catalogue supports service ‘badging’ using this model. In this way, users can distinguish between services that are poorly described and perhaps unlikely to perform reliably, and those with higher quality descriptions and annotations. This encourages service providers to invest more time and effort in annotating their services and improving their documentation. It eases discovery and use of services by end users and scientists.
Within the catalogue we have provided an automated framework for service availability monitoring. Monitoring is performed on a daily basis. Service providers and curators are notified of potential availability problems when these are detected. The statistics collected over time are compiled into service reliability reports to give end users some indication of longer-term reliability of services and to help them choose the most reliable services for their scientific workflows and applications. This public portrayal of service performance information encourages Service Providers to invest time and effort in maintaining and improving the availability and reliability of their offering.
Composing custom programs and workflows with Web services: (A), (B) and (C) in Fig. 1
Today, it is not only reliable and open Web services that are still scarce in ecology, but also easy-to-use applications and orchestration mechanisms that connect such services in a sequence of analytical steps. It takes significant effort to compose and prove an efficient workflow when the sequence of steps is complex—from tens to perhaps many hundreds of individual detailed steps. The inter-relations and transformations between components have to be properly understood to generate confidence in the output result.
In the R language  for example, interaction with servers via the HTTP protocol is built-in, so that a program client for a RESTful service only needs to compose the right request URL and decode the response. Both URL parameters and response formats are exhaustively documented in the Biodiversity Catalogue and off-the-shelf parsers exist for the syntax formats that our services return (e.g., JSON, CSV, domain-specific formats). For SOAP services, the R open source community uses the ‘S SOAP’ package to build more complex, stateful client–server interaction workflows, where an analysis is built up in multiple, small steps rather than a single request/response cycle.
The general-purpose Apache Taverna Workflow tool suite  is a widely used and popular approach to creating, managing and running workflows. With an established community of more than 7500 persons, organised into more than 300 specialised groups, having publicly shared more than 3700 workflows (information correct at March 2016) it represents a rich resource for scientists developing new analysis methods. We have chosen Apache Taverna as the basis for workflows that we developed to build on this already extensive platform, gaining advantage in expertise, familiarity, opportunities for cross-fertilisation and interdisciplinarity that increasingly characterises the science of biodiversity and ecology. With comprehensive capabilities to mix distributed Web Services, local programs/command line tools and other service types (e.g., BioMart queries or R programs) into a single workflow that can be executed locally, on specialised community or institutional computing facilities or “in the cloud”, Taverna was a suitable candidate for the task. We adapted the Taverna tools to meet new requirements we anticipated would arise during the course of this work.
We have developed appropriate interfaces in the Biodiversity Catalogue to invoke Web services directly from the R environment , or from within the Apache Taverna workflow management system . We developed 20 interactive workflows to explore and showcase the utility of the Web services in ecological research. The workflows can be executed through the BioVeL Portal (E) described below. They are summarised in Table 2, with references to scientific studies using these workflows. A more detailed version of Table 2 is available as Additional file 3; also further Additional information describing different scientific studies that have made use of them.
Creating R programs that use Web services: (B) in Fig. 1
Users can interact with the Biodiversity Catalogue and its services in a variety of ways, one of which is by developing their own analysis programs that invoke services in the catalogue. Both the catalogue itself and the services that are advertised in it are exposed through Applications Programming Interfaces (API) that are accessible using standard Internet protocols (HTTP, with RESTful or SOAP functionality). Hence, writing custom analysis code is relatively straightforward in commonly-used programming languages, such as R . The advantage of this way of interacting with the Biodiversity Catalogue services is that users can do this within a development environment (such as RStudio ). This enables them to go through their analysis one step at a time (in a “read-eval-print loop”) visually probing their data as it accumulates. Users can include additional functionalities accessible through Web services  as well as from relevant third-party R packages for biodiversity and ecological analysis; many of which have been developed in recent years. These latter are available, for example via CRAN . The Additional file 1 ‘how-to’ guidelines points to an example of how to create an R program that calls a Web service. Given the popularity of the R programming language in biodiversity and ecology, we expect to see not just ad hoc analysis programs but also published, re-usable analysis libraries written against Web services APIs. The Biodiversity Catalogue provides a single place where such Web services can be found.
Several Web services can be linked together in sequence in an R program to create a ‘work flow’. However, this can rapidly become quite complex. Outputs of one Web service may not match the inputs of the next. Conversions and other needs (such as conditional branching, nesting of sub-flows, parallel execution of multiple similar steps, or waiting asynchronously for a long-running step to complete) all add to the complexity, which has to be managed. Here, workflow management systems, like Apache Taverna  can hide some of the complexity and make workflows easier to create, test and manage. Such systems often offer graphical ‘what you see is what you get’ user interfaces to compose workflows from Web and other kinds of services, such as embedded R programs. Reasonably complex custom workflows can be created (see below) without writing a single line of programming code, which can be attractive for scientists with little or no programming background.
Combined with other capabilities of the BioVeL platform, including transparent access to greater levels of computing capability and capacity for processing large amounts of data, managing the complexity of multiple workflow runs, sharing workflows and provenance, offering data services, etc.) Web services can be applied (i) consistently and (ii) in combination. We have given further examples of potential areas of application where these functions can be combined to support and accelerate new research in the "Discussion" section below (under ‘Towards more comprehensive and global investigations’).
Creating a workflow from an R program: (B) and (C) in Fig. 1
It is possible to convert pre-existing R programs for inclusion into Taverna workflows as discrete ‘R service’ steps. We have developed some recommendations  to make this as easy as possible. We have, for example taken an existing R program that uses data from a local directory and incorporated this into a workflow that generates graphical plots from Ocean Sampling Day (OSD) data  to visualise the metagenomic sequence diversity in ocean water samples. We exposed the inputs and outputs of the R program as ‘ports’ of the corresponding R service, such that the program can be easily re-run using different data. A user could re-use this or another R program, wrapped as a service into their own workflow. Because the workflow is executed on the BioVeL platform, including execution of the R service, there is no need to run R locally on their own computer. This approach gives the possibility to combine R programs and workflows in complementary fashion, the full power of which becomes evident when workflows are embedded as executable objects in 3rd party web sites and web applications (see "Execute a workflow in external applications" section, below).
Building a workflow from Web services: (C) in Fig. 1
Using the Apache Taverna Workbench , we devised workflows meeting scientists’ own needs or fulfilling common needs for routine tasks performed by many scientists in a community. The design and creation of a workflow from Web services requires some programming skills and has often been done by service curators at institutes that also provide Web services.
The Taverna Workbench is a ‘what you see is what you get’ graphical tool, locally installed on the user’s desktop computer that can be used to create and test workflows using a ‘drag and drop’ approach. In the Workbench, users select processing steps from a wide-ranging list of built-in local processing steps and on-line Web services to create a workflow. They do this by dragging and dropping the step into a workflow and linking to its other steps. Each step is in reality an encapsulation of a software tool (an R program, for example) with its own inputs and outputs. Workbench users link the inputs of a step to the outputs from a preceding step and the outputs to the inputs of the next step. Links can be edited when steps are inserted, removed or re-organised. The user can test the workflow by running it locally on their desktop computer or by uploading it to BioVeL Portal (described below).
We have provided a customised version of the Workbench, Taverna Workbench for Biodiversity , configured with a selectable palette of services especially relevant to biodiversity science and ecology. This version provides a direct link to the Biodiversity Catalogue, allowing users to search for the most recent and useful external Web services provided by the community as a whole.
Customising existing workflows: (D) and (C) in Fig. 1
Scientists with programming skills can inspect and modify existing workflows available in the myExperiment workflow library (http://biovel.myexperiment.org), again using the Taverna Workbench tool. There is a direct link to the public myExperiment workflow library (described below), allowing to search for and download existing workflows.
As an example, a scientist used an existing workflow for statistical calculations of differences between ENM output raster files (Table 2, ESW DIFF) to create a new variant that additionally calculates the magnitude and direction of shift in distribution between two model projections. Enhancing the underlying logic (R program in this case) with additional code to compute the weighted centre point of each model projection, and the geographic distance between them was all that was needed. The required data management and visualization resources were already in place, provided by other elements of the existing workflow and the BioSTIF service (Table 1). The ESW DIFF workflow was modified to include the functionality of the new variant.
In a further example: Aphia, the database underlying the World Register of Marine Species (WoRMS)  is a consolidated database for marine species information, containing valid species names, synonyms and vernacular names, higher taxon classification information and extra information such as literature and biogeographic data. Its principal aim is to aid data management, rather than to suggest any taxonomic or phylogenetic opinion on species relationships. As such it represents a resource that is complementary to those already programmed as part of the Data Refinement Workflow (Table 2). After working with the Service Provider to register the service in the Biodiversity Catalogue, we easily modified the Data Refinement Workflow to present the AphiaName lookup service as a choice alongside the Catalogue of Life and GBIF Checklist Bank lookup services when carrying out the taxonomic name resolution stage of the workflow.
Building and using workflow components (not illustrated in Fig. 1)
Packaging a series of related processing steps into a reusable component eases the complexity of building workflows. For example, the task of dynamically defining a geographically bounded area (known as a mask) within which something of interest should be modelled (or selecting from a list of pre-defined masks) involves a lengthy sequence of steps and interactions between a user and a Web service that is used to do the mask creation and selection. To create this from scratch every time it is needed in a workflow would be time-consuming and error-prone. A “create_or_select_mask” component makes it easier to do.
Such components serve as basic building blocks in larger or more complex workflows, making workflows quicker and easier to assemble. We have developed a series of ecological niche modelling (ENM) components that have been mixed and matched for investigating the effects of mixing different spatial resolutions in ENM experiments ; as well as to assemble a jack-knife resampling workflow to study the influence of individual environmental parameters as part of our study on species distribution responses to scenarios of predicted climate change . The packages of population modelling workflows (Table 2) are also based on component families, allowing mix-and-match configuration of population modelling analyses. Well-designed and well-documented sets of workflow components can effectively allow a larger number of scientists without in-depth programming skills to more easily assemble new analytical pipelines.
Discovering workflows: (D) in Fig. 1
As with making Web services available in a directory to encourage discovery and re-use, sharing workflows publicly encourages re-use and adoption of new methods. It makes those methods available to users having less skills or time and effort to create such methods. More importantly, sharing enables more open science, repeatability and reproducibility of science, as well as favouring peer-review of both the methods themselves and results arising from their use.
One mechanism for sharing nurtures a distinctive community of biodiversity workflow practitioners within the well-established myExperiment online workflows repository . This social repository provides workflow publishing, sharing and searching facilities. Within myExperiment we have established a discrete group with its own distinctive branding, where our workflows are shared. The BioVeL group  allows scientists from the biodiversity community to upload their workflows, in silico experiments, results and other published materials. Currently (at the time of writing) the BioVeL domain of myExperiment features almost 40 workflows. Through active participation and collaboration, users can contribute to and benefit from a pool of scientific methods in the biodiversity domain, and be acknowledged when their workflows have been re-used or adapted.
Executing workflows: (E) in Fig. 1
As a part of the BioVeL virtual laboratory, we designed and deployed the BioVeL Portal (http://portal.biovel.eu) , an Internet Web browser based execution environment for workflows. The Portal does not require any local software installations and scientists can use a Web browser interface to upload and execute workflows from myExperiment (or they can choose one already uploaded). Once initiated, users are able to follow the progress of the analysis and interact with it to adjust parameters or to view intermediate results. When satisfied with the final results, a user can share these with others or download them to their local computer. Results can be used as inputs to subsequent work or incorporated into publications, with citation to the workflow and parameters that produced them.
We adopted and adapted SEEK, the systems biology and data model platform  to meet the needs of the biodiversity science and ecology community. We re-branded SEEK for BioVeL and gave it a user interface suited to typical tasks associated with uploading and executing workflows and managing the results of workflow runs. We equipped it to execute workflows on the users’ behalf, for multiple users and multiple workflows simultaneously.
The BioVeL Portal offers functions for discovering, organising and sharing both blueprints for analyses (i.e., workflows) as well as results of analyses (i.e., workflow runs) among collaborators and groups. The Portal provides users with their own personal workspace in which to execute workflows using their own data and to keep their results. Users can manage how their results are shared. At any time, they can share workflows and results publicly, within and between projects, or in groups of individuals. Users can return to their work at any time and pick up where they left off. This ability to create ‘pop-up’ collaborations by inviting individuals into a shared workspace to explore an emerging topic, and to keep track of work offers an immediate way to establish exciting new collaborations with little administrative overhead.
Presently (at the time of writing) there are 50 workflows publicly available within the BioVeL Portal. To support them we have provided a Support Centre, including training materials, documentation (http://wiki.biovel.eu) and helpdesk (mailto:email@example.com) where users can obtain assistance. The expanded version of Table 2 in the Additional file 3 gives full details.
As an example, workflows created for invasive alien species studies  have been frequently re-used in other scientific analyses; for example, to predict potential range shifts of commercially important species under scenarios of climatic change , and to describe the biogeographic range of Asian horseshoe crabs . Here especially, seamless linkage of data access to species occurrence records and environmental data layers, as well as the partly automated cleaning and processing procedures are useful functions when running niche modelling experiments for several species across a large number of parameter settings. The Data Refinement Workflow  has likewise been used in both preparation of niche modelling experiments as well as in analysis of historical changes in benthic community structure . Here especially, the Taxonomic Name Service and data cleaning functions were helpful in resolving synonyms, correcting misspellings, and dealing with other inconsistencies in datasets compiled from different sources.
The Portal also offers functions for data and parameter sweeping. This includes batch processing of large quantities of separate input data using the same parameters (data sweeping) and batch processing the same data using different parameters (parameter sweeping). As example, the niche modelling workflow (Table 2, ENM) has 15 user interaction steps where parameters or files have to be supplied. When repeated manually multiple times this is error-prone. The sweep functions can be used to automate systematic exploration of how data and parameters affect the results in a larger analysis. In such cases the Portal can automatically initiate multiple workflow runs in parallel, significantly reducing the time needed to complete all the planned experiments. It is possible to delegate computing intensive operations to 3rd party computing facilities such as a high performance computing (HPC) centre or a cloud computing service.
Scientists have used the batch processing capability of BioVeL to explore parameter space in models and to generate comparable results for a large number of species. For example, in investigations of present and future distributions of shellfish (Asian Horseshoe Crabs) under predicted climate changes, the technique has been used to generate consensus outputs based on several different, individually executed niche modelling algorithms (for example: MaxEnt, Support Vector Machine and Environmental Distance) to build and evaluate a wide range of models with different combinations of environmental data layers (parameter sweep with 12 different combinations of environmental layers); and to build models for multiple ecologically similar species (data sweep for six intertidal shellfish species). Such calculations, running three modelling algorithms with 12 different environmental datasets for six species (i.e., 216 models) can be concluded in a single day via the Portal.
Execute a workflow in external applications
Finally, BioVeL supports executable workflows to be embedded in other web sites and applications; just like YouTube™ videos can be embedded in web sites. Such embedding would allow, for example a web site giving statistical information about fluctuations in a species population to be rapidly updated as soon as the most recent survey data is entered. Or, it could allow members of the public (e.g., school students) to explore ‘what-if’ scenarios by varying the data and parameter detail without specific knowledge of the workflow executing behind the website. In Scratchpads , 6000+ users have the possibility now to embed workflows into their personal and collaborative Scratchpad websites to repeatedly process their data; as in BioAcoustica  for example. BioAcoustica is an online repository and analysis platform for scientific recordings of wildlife sounds. It embeds a workflow based on an R package that allows scientists contributing data to the site to analyse the sounds.
Distributed computing infrastructure and high performance computing
Although not reported in detail in the present paper, we configured and deployed the underlying information and communications technology (ICT) infrastructure needed to support a multi-party distributed heterogeneous network of biodiversity and ecology Web services (the Biodiversity Service Network), and the execution of workflows simultaneously by multiple users. We offered a pilot operational service. In doing so we utilised different kinds of distributed computing infrastructure, including: Amazon web services (AWS), EGI.eu Federated Cloud/INFN ReCaS Network Computing, SZTAKI Desktop Grid, as well as various localised computer servers under the administration of the partner and contributing organisations. This demonstrates the ability of the BioVeL Web services Network to cope with heterogeneity of underlying infrastructures by adopting a service-oriented computing approach.
We wanted to kick-start familiarisation and application of the workflow approach in biodiversity science and ecology. Our work shows that the Biodiversity Virtual e-Laboratory (BioVeL) is a viable operational and flexible general-purpose approach to collaboratively processing and analysing biodiversity and ecological data. It integrates existing and popular tools and practices from different scientific disciplines to be used in biodiversity and ecological research. This includes functions for: accessing data through curated Web services; performing complex in silico analysis through exposure of R programs, workflows, and batch processing functions; on-line collaboration through sharing of workflows and workflow runs; experiment documentation through reproducibility and repeatability; and computational support via seamless connections to supporting computing infrastructures. Most of these functions do exist today individually and are frequently used by biodiversity scientists and ecologists. However, our platform unites them as key components of large-scale biodiversity and ecological research in a single virtual research environment.
We developed scientifically useful workflows in thematic sub-domains (taxonomy, phylogenetics, metagenomics, niche and population modelling, biogeochemical modelling) useful to address topical questions related to ecosystem functioning and valuation, biospheric carbon sequestration and invasive species management. These topical science areas have real unanswered scientific questions, with a potentially high societal impact arising from new knowledge generated. We applied our workflows to case studies in two of these areas, as well as to case studies more generally in niche modelling and phylogenetics. Our scientific results (Additional file 4) demonstrate that the combination of functions in BioVeL have potential to support biodiversity and ecological research involving large amounts of data and distributed data, tools and researchers in the future.
Strengths and weaknesses
The key criterion for success of the infrastructure and the associated use of Web services is delivering the ability to perform biodiversity and ecology research faster, and/or cheaper, and/or with a higher quality. From the scientists’ perspective, we have seen increased ease of use and improved ability to manage complexity when faced with manipulation and analysis of large amounts of data. The upfront investment to design new workflows pays off not only in the multiple applications of it to different scientific questions and re-uses of it across data and parameter sweeps; but also in terms of time to accomplish work, especially when large analysis can be easily delegated to appropriate computing infrastructures.
Exploiting distributed data resources and processing tools via the Internet opens access to vastly greater computing capacity and analytical capability than is normally available in a desktop or local cluster computer. Our work with the Biome-BGC workflows (see Additional file 4) model and supporting database reused 1100 datasets and 84 parameter sets 84 times, achieving a performance of about 92,000 model runs during 22 days (three simulations per minute on average).
Meeting conditions for reproducibility of work
Wrapping R programs and Web service interactions in workflows removes the repetitiveness, inconsistency and lack of traceability of manual work, while permitting consistent repetition of an experiment. The BioVeL system keeps track of how the analysis was done, documents the research steps and retains the provenance of how the workflow executed. This provenance information helps in recording and tracing back to decisions, reducing time for error discovery and remedy; as well as formalization for reporting. It is these consistent processing and tracking features (rather than speed of execution per se) that are a principal advantage when dealing with large amounts of data, and when running many algorithms and different parameter settings across that data. They give an investigator the ability to document, overview, share and collaboratively evaluate the results from a complex large-scale study.
A progressive drive towards more open research, including with greater reproducibility [62, 63] and stronger emphasis on ‘elevating the status of code in ecology’  is leading journal publishers (including those of the present article, BioMed Central) to make it a condition of publication that data (and increasingly, software) should be accessible and easy to scrutinise. As noted in a BMC Ecology editorial  the idea that the data underlying a study should be available for validation of conclusions is not unreasonable. By implication, “…readily reproducible materials… freely available…” includes the workflows and software that have been used for preparation and analysis of that data. Using the BioVeL ecosystem is an easy way of meeting such conditions.
Increased levels of inter-disciplinary working
The infrastructure enables increased levels of inter-disciplinary working and more scalable scientific investigations. The first generation of publications resulting from the e-laboratory is encouraging and shows that BioVeL services start providing these features. The majority of the users of ecological niche modelling workflows (for example) may not be experts in this field. They can be scientists with backgrounds in ecology, systematics, and environmental sciences that use the workflows to become familiar with new analytical methods [33, 36, 54]. Similarly, the taxonomic, phylogenetic and metagenomic services have been used by scientists to complement their existing analytical expertise with that from another field [36, 44]. A further example: Amplicon-based metagenomics approaches have been widely used to investigate both environmental and host associated microbial communities. The BioMaS (Bioinformatic analysis of Metagenomic ampliconS) Web service (Table 2; ) offers a way to simply and accurately characterize fungal and prokaryotic communities, overcoming the necessity of computer-science skills to set up bioinformatics workflows. This is opening the field to a wide range of researchers, such as molecular biologists  and ecologists .
Towards more comprehensive and global investigations
The principal BioVeL functionalities support more comprehensive and global investigations of biodiversity patterns and ecological processes. Such investigations are not impossible today but they are expensive and often can only be addressed with large and resourceful scientific networks. Exploiting such scalability is particularly attractive, for example to prepare and verify large-scale data products relating to the essential biodiversity variables (EBV) ; for phyloclimatic investigations ; and for characterisation of biogeographic regions . In addition, complex predictive approaches that couple mechanistic with statistical models may benefit from the use of the BioVeL environment . All these kinds of processing usually require integration of distributed biological, climate and environmental data, drawn from public databases as well as personal sources. They depend on a wide range of analytical capabilities, computational power and, most importantly the combined knowledge of a large number of experts. The BioVeL platform can connect these critical resources on the fly. In conjunction with an easy-to-use interface (the Portal) they can be used to dynamically create ad hoc scientific networks and cross-disciplinary collaborations fast. In the absence of dedicated funding it is a mechanism that can help scientists to react more quickly to newly emerging socio-environmental problems. The infrastructure is increasingly used for this purpose of ‘next-generation action ecology’ .
Dependency on supporting infrastructure and robust Web services
One apparent drawback of the approach we describe is dependency on the ready availability of robust infrastructure to provide access to data and to processing capabilities. This is out of the control of the end-user scientists but it is a matter for service providers. It is the same issue we face as consumer users of the Internet, whereby we rely on a well-developed portfolio of robust related services; for example, for making our travel arrangements with airlines, rental cars and hotels. In the biodiversity and ecology domain this is not the case. The portfolio of services is not yet well developed. There are only a limited number of robust large-scale service providers thus far (GBIF, EMBL-EBI, OBIS, PANGAEA to name just four examples) and not many smaller ones. Compare this with the life sciences community, where more than 1000 Web services from more than 250 service providers are listed in the BioCatalogue . By promoting the Biodiversity Catalogue  as the well-founded one-stop shop to keep track of high-quality Web services as they appear; and annotating entries in the catalogue to document their capabilities we are hoping to encourage steps towards greater maturity. As with all software, the services and workflows, and the platforms on which they run have to be maintained. There is a cost associated with that. Projects like Wf4ever (“Workflow for Ever”)  have examined some of the challenges associated with preserving scientific experiments in data-intensive science but long-term it is a community responsibility that still has to be addressed.
Results in context
Prototypes to operational service
Historical projects such the UK’s BBSRC-funded Biodiversity World project, 2003–2006  and the USA’s NSF-funded SEEK project 2002–2007  (not to be confused with the SEEK platform for systems biology) successfully explored the potential of automated workflow systems for large-scale biodiversity studies. Moving from concept-proving studies towards a reliable infrastructure supporting collaboration is a substantial challenge. In the long-run such infrastructure has to robustly serve many thousands of users simultaneously.
With BioVeL we offer a pilot-scale operational service, delivered continuously and collaboratively by multiple partner organisations. This “Biodiversity Commons” of workflows, services and technology products can be used by anyone. Embedding elements of it within third party applications and contexts such as Scratchpads , Jupyter/iPython Notebooks , data analysis for Ocean Sampling Day collection events , national level biodiversity information infrastructures  and biodiversity observation networks has a multiplier effect, making it possible for all users of those wider communities and others to execute and exploit the power of workflows.
The underlying SEEK platform  on which BioVeL is based (not to be confused with the SEEK project mentioned above) is designed fundamentally to assist scientists to organise their digital data analysis work. As well as supporting execution of workflows, it allows them to describe, manage and execute their projects. These normally consist of experiments, datasets, models, and results. It helps scientists by gathering and organising pieces of information related to these different artefacts into different categories and making links between them; namely: yellow pages (programmes, people, projects, institutions); experiments (investigations, studies, assays); assets (datasets, models, standard operating procedures, publications); and activities (presentations, events). Not all the functionality of SEEK is presently enabled in the BioVeL Portal variant but in future it can be enabled as the needs of the community grow.
Global research infrastructures
Globally, organisations with data and processing facilities across the world are working to deliver research infrastructure services to their respective scientific user communities. Initiatives in Europe (LifeWatch), Australia (Atlas of Living Australia), Brazil (speciesLink network, SiBBr Brazilian Biodiversity Information System), China (Academy of Sciences National Specimen Information Infrastructure and the World Federation of Culture Collections), South Africa (SANBI Integrated Biodiversity Information System), USA (DataONE and NEON) as well as GBIF, Catalogue of Life, Encyclopedia of Life, Biodiversity Heritage Library, and others are all mutually interdependent. They are driven not only by the direct needs of curiosity science but also more and more by the science needs of global policy initiatives. All research infrastructure operators recognise the need to remove barriers to global interoperability through common approaches based on interoperable Web services and promoting the development, sharing and use of workflows . Our work is relevant to and supports this goal.
IPBES, GEO BON, and essential biodiversity variables
The Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES) has to provide assessments of the state of the environment . Guidelines for authors of assessments focus on several areas highly relevant in the context of the present paper: (i) improving access to data, information and knowledge of all types; (ii) managing data uncertainty and quality; and (iii) performing various model simulations and scenario-based analysis of future developments . Additionally, some key principles and practices are given to ensure respect for and to consistently apply transparency at all steps of data collection, selection, analysis and archiving. This is so that IPBES can enable replication of results and informed feedback on assessments; comparability across scales and time; and use of systematic methodology and shared approach in all steps of the assessment process. The workflow approach, applied via BioVeL tools and infrastructure with specific additional developments to support Essential Biodiversity Variables in conjunction with other partners from the Group on Earth Observations Biodiversity Observation Network (GEO BON) would be a very progressive move to fulfil these requirements .
Towards wider use of workflows
Tools for creating, executing and sharing workflows to process and analyse scientific data (see third paragraph of the introduction) have been around for 15 years. Most of these started life as desktop tools. Indeed, Kepler was a product of the previously mentioned SEEK project , with origins in ecological science. Despite variable usage across disciplines the cumulative experience is that the general approach of configurable, flexible workflows to assist the process of transforming, analysing and modelling with large amounts of data is well accepted. Workflows as a paradigm for orchestrating disparate capabilities to pursue large-scale data intensive ecological science are an important next step for the community. They represent “primacy of method” for a community evolving towards a new research culture that is increasingly dependent on working collaboratively, exchanging and aggregating data and automating analyses [63, 81]. They balance shareability, repeatability and flexibility with simplicity.
In conclusion, we have presented a virtual laboratory that unites critical functions necessary for supporting complex and data intensive biodiversity science and ecological research in the future. We have created and deployed multiple Web services and ‘off-the-shelf’ packs of pre-defined workflows that meet the specific needs for several types of scientific study in biodiversity science and ecology. We have made these available respectively through a catalogue of services, the Biodiversity Catalogue and via a public repository of workflows, myExperiment. Each part can be used independently of the others or as an integrated part of the platform as a whole. BioVeL is operational and we have provided guidelines for its use (Additional file 1). We can refer (via Additional file 4) to many scientific studies that have used and are using the platform. We have raised awareness of what is possible and have laid foundations for further adoption and convergence activities as more ecologists encounter the worlds of big data and open science.
We foresee two main directions of future development
Firstly, building complete, flexible, independent virtual laboratories will become more commonplace. Scientists want to be in control of their own real physical laboratories and there is no reason to assume they will not want to be in control of their own virtual laboratories for data processing and analysis. As with their physical laboratories, scientists will not want to build all elements from scratch. They will wish to take advantage of proven ready-built workflows and workflow components built and tested by trusted suppliers. Such workflows and components are part of an emerging Biodiversity Commons those labs can draw upon. We already have the first cases where scientists use BioVeL to expose and share their own analytical assets, and begin to pool and aggregate tools developed by the community rather than for the community. Capabilities for data management have been built into the core of BioVeL because the users requested it. Combined, such functionality provides a comprehensive collaborative platform that supports the needs of modern-day reproducible digital science.
The second direction extends towards building the sufficient base of robust data and computational services in biodiversity and ecological sciences (Web services, R programs and command line tools) that can be combined to automate multi-stage processing and analysis tasks. This will give scientists the freedom to compose sequences of processing steps to perform the scientific tasks they know and are familiar with today. At the same time the approach retains a dual flexibility. It permits the addition of new capabilities as those develop and evolve. It also allows for composing capabilities in ways that cannot presently be foreseen; thus meeting scientists’ needs of the future.
application programming interface
bioinformatic analysis of metagenomic ampliconS
biodiversity virtual e-laboratory
Bayesian phylogenetic inference
Species 2000/ITIS catalogue of life
comprehensive R archive network
Centro de Referência em Informação Ambiental
data observation network for earth
data refinement workflow
European Bioinformatics Institute
essential biodiversity variable
European Molecular Biology Laboratory
EMBL European Bioinformatics Institute
ecological niche modelling
ecosystem service indicator
ENM statistical difference workflow
Global Biodiversity Information Facility
- GEO BON:
Group on Earth Observations Biodiversity Observation Network
high performance computing
Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services
integral projection model
Laboratory of Neuro Imaging
microbial metagenomic trait calculation and statistical analysis workflow
matrix projection model
multiple sequence alignment based on PFAM accessed domain information
National Ecological Observatory Network
Ocean Biogeographic Information System
Ocean Sampling Day
operational taxonomic unit
persistent uniform resource locators
representational state transfer (a mechanism for communicating between application programs and some web services cf. SOAP)
South African National Biodiversity Institute
science environment for ecological knowledge (historical project)
systems biology and data model platform (unrelated to SEEK, the project)
simple object access protocol (a mechanism for communicating between application programs and some web services cf. REST)
virtual research environment
World Register of Marine Species
Evans MR. Modelling ecological systems in a changing world. Philos Trans R Soc Lond B Biol Sci. 2012;367:181–90. doi:10.1098/rstb.2011.0172.
Evans MR, Bithell M, Cornell SJ, Dall SRX, Díaz S, Emmott S, et al. Predictive systems ecology. Proc Biol Sci. 2013;280:20131452. doi:10.1098/rspb.2013.1452.
Purves D, Scharlemann J, Harfoot M, Newbold T, Tittensor DP, Hutton J, et al. Ecosystems: time to model all life on Earth. Nature. 2013;493:295–7. doi:10.1038/493295a.
Díaz S, Demissew S, Carabias J, Joly C, Lonsdale M, Ash N, et al. The IPBES conceptual framework—connecting nature and people. Curr Opin Environ Sustain. 2015;14:1–16. doi:10.1016/j.cosust.2014.11.002.
Hampton SE, Strasser CA, Tewksbury JJ, Gram WK, Budden AE, Batcheller AL, et al. Big data and the future of ecology. Front Ecol Environ. 2013;11:156–62. doi:10.1890/120103.
Michener WK, Jones MB. Ecoinformatics: supporting ecology as a data-intensive science. Trends Ecol Evol. 2012;27:85–93. doi:10.1016/j.tree.2011.11.016.
Koureas D, Arvanitidis C, Belbin L, Berendsohn W, Damgaard C, Groom Q, et al. Community engagement: the “last mile” challenge for European research e-infrastructures. Res Ideas Outcomes. 2016;2:e9933.
Altintas I, Berkley C, Jaeger E, Jones M, Ludascher B, Mock S. Kepler: an extensible system for design and execution of scientific workflows. In: Proceedings 16th international conference on scientific and statistical database management. IEEE. 2004:423–424. doi:10.1109/SSDM.2004.1311241.
Deelman E, Vahi K, Juve G, Rynge M, Callaghan S, Maechling PJ, et al. Pegasus, a workflow management system for science automation. Futur Gener Comput Syst. 2015;46:17–35. doi:10.1016/j.future.2014.10.008.
Wolstencroft K, Haines R, Fellows D, Williams A, Withers D, Owen S, et al. The Taverna workflow suite: designing and executing workflows of web services on the desktop, web or in the cloud. Nucleic Acids Res. 2013;41:W557–61. doi:10.1093/nar/gkt328.
Callahan SP, Freire J, Santos E, Scheidegger CE, Silva CT. Managing the evolution of dataflows with VisTrails. In: 22nd international conference on data engineering workshops (ICDEW’06). IEEE. 2006:71. doi:10.1109/ICDEW.2006.75.
Berthold MR, Cebron N, Dill F, Gabriel TR, Kötter T, Meinl T, Ohl P, Thiel K, Wiswedel B. KNIME-the Konstanz information miner: version 2.0 and beyond. In: ACM SIGKDD explorations Newsletter. vol. 16. New York: ACM. 2009. p. 26–31.
Goecks J, Nekrutenko A, Taylor J. Galaxy: a comprehensive approach for supporting accessible, reproducible, and transparent computational research in the life sciences. Genome Biol. 2010;11:R86. doi:10.1186/gb-2010-11-8-r86.
Hofmann M, Klinkenberg R. RapidMiner: data mining use cases and business analytics applications. Boca Raton: CRC Press, Taylor & Francis Group; 2013.
Fisher P, Hedeler C. A systematic strategy for large-scale analysis of genotype–phenotype correlations: identification of candidate genes involved in African trypanosomiasis. Nucleic Acids Res. 2007;35:5625–33. doi:10.1093/nar/gkm623.
Bentley RD, Csillaghy A, Aboudarham J, Jacquey C, Hapgood MA, Bocchialini K, et al. HELIO: the heliophysics integrated observatory. Adv Space Res. 2011;47:2235–9. doi:10.1016/j.asr.2010.02.006.
Hardy B, Douglas N, Helma C, Rautenberg M, Jeliazkova N, Jeliazkov V, et al. Collaborative development of predictive toxicology applications. J Cheminform. 2010;2:7. doi:10.1186/1758-2946-2-7.
Rex DE, Ma JQ, Toga AW. The LONI pipeline processing environment. Neuroimage. 2003;19:1033–48. doi:10.1016/S1053-8119(03)00185-X.
Lu Y, Yue T, Wang C, Wang Q. Workflow-based spatial modeling environment and its application in food provisioning services of grassland ecosystem. In: 2010 18th international conference on geoinformatics. IEEE. 2010:1–6. doi:10.1109/GEOINFORMATICS.2010.5567853.
Krüger F, Clare EL, Greif S, Siemers BM, Symondson WOC, Sommer RS. An integrative approach to detect subtle trophic niche differentiation in the sympatric trawling bat species Myotis dasycneme and Myotis daubentonii. Mol Ecol. 2014;23:3657–71. doi:10.1111/mec.12512.
Michener W, Beach J, Bowers S, Downey L, Jones M, Ludäscher B, et al. Data integration and workflow solutions for ecology. In: Proceedings, 2nd International Workshop on Data Integration in the Life Sciences July 20-22, 2005 Univ Calif, San Diego, San Diego, USA. Lecture Notes in Computer Science. Vol. 3615. Berlin: Springer; 2005. p. 321–324.
Pennington D, Higgins D, Peterson A, Jones M, Ludäscher B, Bowers S. Ecological niche modeling using the Kepler workflow system. In: Taylor I, Deelman E, Gannon D, Shields M, editors. Workflows for e-Science scientific workflows for grids. London: Springer; 2007. p. 91–108. doi:10.1007/978-1-84628-757-2_7.
Jarnevich CS, Holcombe TR, Bella EM, Carlson ML, Graziano G, Lamb M, et al. Cross-scale assessment of potential habitat shifts in a rapidly changing climate. Invasive Plant Sci Manag. 2014;7:491–502. doi:10.1614/IPSM-D-13-00071.1.
Dou L, Zinn D, McPhillips T, Köhler S, Riddle S, Bowers S, et al. Scientific workflow design 2.0: demonstrating streaming data collections in Kepler. In: IEEE 27th international conference on Data engineering (ICDE). IEEE. 2011. doi:10.1109/ICDE.2011.5767938.
Dou L, Cao G, Morris P, Morris R, Ludäscher B, Macklin J, et al. Kurator: a Kepler package for data curation workflows. Proc Comput Sci. 2012;9:1614–9. doi:10.1016/j.procs.2012.04.177.
BioVeL portal. http://portal.biovel.eu/. Accessed 21 Mar 2016.
Papazoglou MP, Georgakopoulos D. Introduction: service-oriented computing. Commun ACM. 2003;46:24. doi:10.1145/944217.944233.
De Giovanni R, Torres E, Amaral R, Blanquer I, Rebello V, Canhos V. OMWS: a web service interface for ecological niche modelling. Biodivers Inform. 2015;10:35–44. doi:10.17161/bi.v10i0.4853.
Biodiversity catalogue. http://www.biodiversitycatalogue.org/. Accessed 4 Apr 2015.
Bhagat J, Tanoh F, Nzuobontane E, Laurent T, Orlowski J, Roos M, et al. BioCatalogue: a universal catalogue of web services for the life sciences. Nucleic Acids Res. 2010;38((Web Server issue)):W689–94. doi:10.1093/nar/gkq394.
Ihaka R, Gentleman R. R: a language for data analysis and graphics. J Comput Graph Stat. 1996;5:299–314. doi:10.1080/10618600.1996.10474713.
Racine JS. RStudio: a platform-independent IDE for R and sweave. J Appl Econom. 2012;27:167–72. doi:10.1002/jae.1278.
Leidenberger S, Obst M, Kulawik R, Stelzer K, Heyer K, Hardisty A, et al. Evaluating the potential of ecological niche modelling as a component in marine non-indigenous species risk assessments. Mar Pollut Bull. 2015;97:470–87. doi:10.1016/j.marpolbul.2015.04.033.
Obst M, Vicario S, Berggren M, Karlsson A, Lundin K, Haines R, et al. Marine long-term biodiversity assessment indicates loss of species richness in the Skagerrak and Kattegat region. Mar Biodivers (In review).
De Giovanni R, Williams AR, Vera Hernández E, Kulawik R, Fernandez FQ, Hardisty AR. ENM components: a new set of web service-based workflow components for ecological niche modelling. Ecography (Cop). 2015;. doi:10.1111/ecog.01552.
Laugen AT, Hollander J, Obst M, Strand Å. The Pacific Oyster (Crassostrea gigas) invasion in Scandinavian coastal waters in a changing climate: impact on local ecosystem services. In: Canning-Clode J, editor. Biological invasions in aquatic and terrestrial systems: biogeography, ecological impacts, predictions, and management. Berlin: De Gruyter Open; 2015. p. 230–52.
Hidy D, Barcza Z, Haszpra L, Churkina G, Pintér K, Nagy Z. Development of the Biome-BGC model for simulation of managed herbaceous ecosystems. Ecol Model. 2012;226:99–119. doi:10.1016/j.ecolmodel.2011.11.008.
Sándor R, Ma S, Acutis M, Barcza Z, Ben Touhami H, Doro L, et al. Uncertainty in simulating biomass yield and carbon-water fluxes from grasslands under climate change. Adv Anim Biosci. 2015;6:49–51.
Sándor R, Barcza Z, Hidy D, Lellei-Kovács E, Ma S, Bellocchi G. Modelling of grassland fluxes in Europe: evaluation of two biogeochemical models. Agric Ecosyst Environ. 2016;215:1–19. doi:10.1016/j.agee.2015.09.001.
Sándor R, Acutis M, Barcza Z, Doro L, Hidy D, Köchy M, et al. Multi-model simulation of soil temperature, soil water content and biomass in Euro-Mediterranean grasslands: uncertainties and ensemble performance. European. Eur J Agron. 2016; (In Press). doi:10.1016/j.eja.2016.06.006.
Kopf A, Bicak M, Kottmann R, Schnetzer J, Kostadinov I, Lehmann K, et al. The ocean sampling day consortium. Gigascience. 2015;4:27. doi:10.1186/s13742-015-0066-5.
Manzari C, Fosso B, Marzano M, Annese A, Caprioli R, D’Erchia AM, et al. The influence of invasive jellyfish blooms on the aquatic microbiome in a coastal lagoon (Varano, SE Italy) detected by an Illumina-based deep sequencing strategy. Biol Invasions. 2014;17:923–40. doi:10.1007/s10530-014-0810-2.
Fosso B, Santamaria M, Marzano M, Alonso-Alemany D, Valiente G, Donvito G, et al. BioMaS: a modular pipeline for Bioinformatic analysis of Metagenomic AmpliconS. BMC Bioinformatics. 2015;16:203. doi:10.1186/s12859-015-0595-z.
Sandionigi A, Vicario S, Prosdocimi EM, Galimberti A, Ferri E, Bruno A, et al. Towards a better understanding of Apis mellifera and Varroa destructor microbiomes: introducing “phyloh” as a novel phylogenetic diversity analysis tool. Mol Ecol Resour. 2014;15:697–710. doi:10.1111/1755-0998.12341.
Antonelli A, Hettling H, Condamine FL, Vos K, Nilsson RH, Sanderson MJ, et al. Towards a self-updating platform for estimating rates of speciation and migration, ages, and relationships of taxa (SUPERSMART). Syst Biol. 2016. doi:10.1093/sysbio/syw066.
Balech B, Vicario S, Donvito G, Monaco A, Notarangelo P, Pesole G. MSA-PAD: DNA multiple sequence alignment framework based on PFAM accessed domain information. Bioinformatics. 2015;31:2571–3. doi:10.1093/bioinformatics/btv141.
Delić D, Balech B, Radulović M, Lolić B, Karačić A, Vukosavljević V, et al. Vmp1 and stamp genes variability of “Candidatus phytoplasma solani” in Bosnian and Herzegovinian grapevine. Eur J Plant Pathol. 2016;45:221–5. doi:10.1007/s10658-015-0828-z.
RStudio integrated development environment. https://www.rstudio.com/. Accessed 15 Jul 2016.
CRAN task view: web technologies and services. https://cran.r-project.org/web/views/WebTechnologies.html. Accessed 16 Oct 2015.
CRAN task view: analysis of ecological and environmental data. https://cran.r-project.org/web/views/Environmetrics.html. Accessed 22 Oct 2015.
Creating a workflow from an R script. https://wiki.biovel.eu/x/iYSz. Accessed 28 Aug2015.
Taverna workbench for biodiversity. http://www.taverna.org.uk/download/workbench/2-5/biodiversity/. Accessed 16 Jul 2016.
World register of marine species (WoRMS). http://www.marinespecies.org/. Accessed 21 Mar 2016.
Leidenberger S, De Giovanni R, Kulawik R, Williams AR, Bourlat SJ. Mapping present and future potential distribution patterns for a meso-grazer guild in the Baltic Sea. J Biogeogr. 2015;42:241–54. doi:10.1111/jbi.12395.
De Roure D, Goble C, Stevens R. The design and realisation of the myExperiment virtual research environment for social sharing of workflows. Futur Gener Comput Syst. 2009;25:561–7. doi:10.1016/j.future.2008.06.010.
myExperiment BioVeL group. http://biovel.myexperiment.org/. Accessed 21 Mar 2016.
Wolstencroft K, Owen S, Krebs O, Nguyen Q, Stanford NJ, Golebiewski M, et al. SEEK: a systems biology data and model management platform. BMC Syst Biol. 2015;9:33. doi:10.1186/s12918-015-0174-y.
Funch P, Obst M, Quevedo F. et al. Present and future distributions of horseshoe crabs under predicted climate changes. The third international workshop on the science and conservation of horseshoe crabs, June 15–19, 2015, Saikai national park kujukushima, Sasebo-City, Nagasaki, Japan. http://forskningsdatabasen.dk/en/catalog/2297666567. Accessed 18 Oct 2016.
Mathew C, Güntsch A, Obst M, Vicario S, Haines R, Williams AR, et al. A semi-automated workflow for biodiversity data retrieval, cleaning, and quality control. Biodivers Data J. 2014;2:e4221. doi:10.3897/BDJ.2.e4221.
Haines R, Goble C, Rycroft S, Smith V. Integrating taverna player into scratchpads. Manchester: University of Manchester; 2014. http://zenodo.org/record/10871. Accessed 20 Apr 2015.
Baker E, Price BW, Rycroft SD, Hill J, Smith VS. BioAcoustica: a free and open repository and analysis platform for bioacoustics. Database. 2015;2015:bav054. doi:10.1093/database/bav054.
The royal society. Science as an open enterprise. Final report june. 2012. http://royalsociety.org/uploadedFiles/Royal_Society_Content/policy/projects/. Accessed 1 Sept 2016.
Hampton SE, Anderson SS, Bagby SC, Gries C, Han X, Hart EM, et al. The Tao of open science for ecology. Ecosphere. 2015;6:1–13. doi:10.1890/ES14-00402.1.
Mislan KAS, Heer JM, White EP. Elevating the status of code in ecology. Trends Ecol Evol. 2016;31:4–7. doi:10.1016/j.tree.2015.11.006.
Kenall A, Harold S, Foote C. An open future for ecological and evolutionary data? BMC Ecol. 2014;14:10. doi:10.1186/1472-6785-14-10.
Rigoni R, Fontana E, Guglielmetti S, Fosso B, D’Erchia AM, Maina V, et al. Intestinal microbiota sustains inflammation and autoimmunity induced by hypomorphic RAG defects. J Exp Med. 2016;213:355–75. doi:10.1084/jem.20151116.
Pereira HM, Ferrier S, Walters M, Geller GN, Jongman RHG, Scholes RJ, et al. Essential biodiversity variables. Science. 2013;339:277–8. doi:10.1126/science.1229931.
Verbruggen H, Tyberghein L, Pauly K, Vlaeminck C, Van Nieuwenhuyze K, Kooistra WHCF, et al. Macroecology meets macroevolution: evolutionary niche dynamics in the seaweed Halimeda. Glob Ecol Biogeogr. 2009;18:393–405. doi:10.1111/j.1466-8238.2009.00463.x.
Vilhena DA, Antonelli A. A network approach for identifying and delimiting biogeographical regions. Nat Commun. 2015;6:6848. doi:10.1038/ncomms7848.
Kearney MR, Wintle BA, Porter WP. Correlative and mechanistic models of species distribution provide congruent forecasts under climate change. Conserv Lett. 2010;3:203–13. doi:10.1111/j.1755-263X.2010.00097.x.
White RL, Sutton AE, Salguero-Gómez R, Bray TC, Campbell H, Cieraad E, et al. The next generation of action ecology: novel approaches towards global ecological research. Ecosphere. 2015;6:1–16. doi:10.1890/ES14-00485.1.
Belhajjame K, Zhao J, Garijo D, Hettne K, Palma R, Corcho Ó, et al. The research object suite of ontologies: sharing and exchanging research data and methods on the open web. 2014. arXiv:1401.4307.
Pahwa JS, Brewer P, Sutton T, Yesson C, Burgess M, Xu X, et al. Biodiversity World: a problem-solving environment for analysing biodiversity patterns. In: Sixth IEEE international symposium on cluster computing and the grid (CCGRID’06). IEEE. 2006:1. doi:10.1109/CCGRID.2006.23.
Michener W, Beach J, Jones M, Ludäscher B, Pennington D, Pereira R, et al. A knowledge environment for the biodiversity and ecological sciences. J Intell Inf Syst. 2007;29:111–26. doi:10.1007/s10844-006-0034-8.
Smith VS, Rycroft SD, Brake I, Scott B, Baker E, Livermore L, et al. Scratchpads 2.0: a virtual research environment supporting scholarly collaboration, communication and data publication in biodiversity science. Zookeys. 2011;150:53–70. doi:10.3897/zookeys.150.2193.
Pérez F, Granger B. IPython: a system for interactive scientific computing. Comput Sci Eng. 2007;9:21–9. doi:10.1109/MCSE.2007.53.
Gärdenfors U, Jönsson M, Obst M, Wremp AM, Kindvall O, Nilsson J. Swedish LifeWatch ─ a biodiversity infrastructure integrating and reusing data from citizen science, monitoring and research. Hum Comput. 2014;1:147–61. doi:10.15346/hc.v1i2.6.
García EA, Bellisari L, De Leo F, Hardisty A, Keuchkerian S, Konijn J, et al. Flock together with CReATIVE-B: a roadmap of global research data infrastructures supporting biodiversity and ecosystem science. 2014. http://orca.cf.ac.uk/88151/. Accessed 21 Mar 2016.
IPBES Secretariat. Guide on the production and integration of assessments from and across all scales. 2014. http://www.ipbes.net/work-programme/guide-production-assessments. Accessed 21 Mar 2016.
Kissling WD, Hardisty A, García EA, Santamaria M, De Leo F, Pesole G, et al. Towards global interoperability for supporting biodiversity research on essential biodiversity variables (EBVs). Biodiversity. 2015:1–9. doi:10.1080/14888386.2015.1068709.
Goble C, De Roure D. The impact of workflow tools on data-centric research. In: Hey T, Tansley S, Tolle K, editors. The fourth paradigm: data intensive scientific discovery. Redmond: Microsoft Research; 2009. p. 137–46.
The study was conceived and designed by AH, MO and CG with assistance from FH, AP, HS, YdJ and SV. It was directed by AH, MO, CG, EP and HS. All authors contributed to the detailed implementation of the work, to its reporting and to writing parts of the present article and Additional information. All authors read and approved the final manuscript.
More than 80 persons across 15 partner organisations have been involved in the work over 4 years as part of the BioVeL consortium. Without the contributions of each of them we would not have arrived at the position of being able to offer a pilot service to the scientific community for in silico digital biodiversity and ecosystems research. We thank you for your contribution. We acknowledge the contribution of Yannick Legré, who contributed to the conception and design of the work but ultimately was unable to participate in the project.
We acknowledge the helpful support and constructive criticism we received during the validation stage of our work from A. Townsend Peterson and Jorge Soberón (both of Biodiversity Institute and Natural History Museum, University of Kansas), Robert Guralnick (Florida Museum of Natural History, University of Florida) and Mark P. Schildhauer (National Center for Ecological Analysis and Synthesis, University of California). They gave us the benefit of their expertise and experience.
Finally, we are grateful for the continuing support for our ideas from among our friends in the wider community of biodiversity and ecological informatics specialists around the world, and from those scientists who “get” the idea of e-Science. They number more than 400 persons. Together we are on a journey.
The authors declare that they have no competing interests.
Availability of data and materials, including guidelines for use
The web services and workflows referred to in the text, and particularly in Tables 1 and 2 and their expanded versions in the Additional information are available via their specified urls in the text. Collectively, they are available in the myExperiment workflow library (http://biovel.myexperiment.org) and the appropriate documentation is available in the BioVeL documentation wiki (http://wiki.biovel.eu). Direct Documentation links are also available via the Additional information tables. Additional file 1 provides pointers to other resources for the various different tasks illustrated in Fig. 1.
The work reported in this article has been performed by the Biodiversity Virtual e-Laboratory (BioVeL) project (http://www.biovel.eu) funded by the European Union 7th Framework Programme, Grant no. 283359 (2011–2014). The work was furthermore supported by the Swedish LifeWatch project funded by the Swedish Research Council (Grant no. 829-2009-6278). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the present article.
About this article
- Biodiversity science
- Computing software
- Virtual laboratory
- Biodiversity virtual e-laboratory
- Data processing