Silicon uptake by a pasture grass experiencing simulated grazing is greatest under elevated precipitation

Rain-exclusion shelters

Rain-exclusion shelters (249 cm × 188 cm) located at the Hawkesbury campus of Western Sydney University near Richmond, NSW (latitude − 33.609396, longitude 150.737800), as described by Johnson et al. [29], were used to exclude 100% of ambient rainfall from four mesocosms beneath each of 18 shelters. Mesocosm pots (41 cm × 41 cm × 31 cm) were arranged in a 2 × 2 formation and dug into the ground so that the rim of the pot was flush with the soil surface. Each of the 72 mesocosms was filled with the excavated soil (chemical composition defined in Additional file 1: Table S1; methods from GE Rayment and DJ Lyons [30]), which was air-dried and sieved to < 4 mm.

Experimental procedure

Six Microlaena stipoides (var. Burra) seeds were sown and grown in 288 individual seed cells (38 × 57 mm) under field conditions. After 4 weeks (on 26 September 2016), four cells were transplanted into each mesocosm pot. Shelters were assigned at random to one of three rainfall treatments giving six shelters per rainfall regime. The ambient treatment was set at 65 mm per month, which was the average precipitation in Richmond between September and November over the previous 30 years (Bureau of Meteorology, Australia). The drought and elevated precipitation treatments comprised 50% and 150% of the ambient rainfall amounts, respectively. This translated to an application of 586 mL (ambient precipitation), 293 mL (drought) and 878 mL (elevated precipitation) of rainfall water collected at the site three times per week. Soil moisture measurements were taken weekly using a 12 cm Hydrosense II probe (Campbell Scientific, Queensland, Australia).

Each of two of the four mesocosms underneath each shelter were supplemented with Si by dissolving 146.5 mg of sodium metasilicate (Na₂SiO₃.9H₂O) into the water applied under each watering regime. Four weeks after transplantation, grasses in two of the four mesocosms (one with Si applied and one without) were clipped, using hand shears, to leave approximately 40% of their aboveground biomass. The factorial treatment design under each shelter therefore comprised one mesocosm with Si applied alone (Si), one mesocosm with clipped grasses alone (Clipped), one mesocosm with both treatments applied (Si + Clipped), and one mesocosm with neither (Control; Fig. 1a). Plant heights and tiller numbers were recorded every 2 weeks throughout the experiment and whole plants were harvested 7 weeks after transplanting (when plants were 11 weeks old). Soil samples were taken from the centre of each mesocosm at a depth of approximately 5 cm and oven-dried. Roots were separated from shoots and frozen at − 20 °C, then oven-dried and weighed prior to chemical analysis.

Chemical analyses

Dried root and shoot material and soil samples were ball-milled to a fine powder. Plant material from each mesocosm was pooled to provide enough dried material to conduct chemical analyses, giving one root and shoot sample per mesocosm (i.e. 72 roots and 72 shoots). Carbon (C) and nitrogen (N) concentrations were determined with a Carlo Erba CE1110 elemental analyser and total Si concentrations (% of dry soil mass) were determined with an X-ray fluorescence spectrometer (Epsilon-3x, PANalytical, EA Almelo, The Netherlands), as described in I Hiltpold, L Demarta, SN Johnson, BD Moore, SA Power and C Mitchell [31]. The “uptake efficiency” of Si (mg Si per mg root mass) was calculated by dividing the total content of Si in foliar tissue (i.e. % Si × shoot mass) by the total root mass.

Statistical analyses

Soil moisture and plant growth

Soil moisture was significantly affected by the interaction between rainfall and Si-application (Table 1), whereby soil moisture retention increased when Si was applied to the soil, but only under ambient and elevated precipitation conditions (Fig. 1b). Before the clipping treatment was applied, heights were significantly lower under drought compared with ambient and elevated precipitation and increased in Si-supplemented soils compared with untreated soils (Table 1). In general, plants under drought had significantly fewer tillers than those under ambient and elevated precipitation. Similarly, plants that were clipped had fewer tillers than those that were not clipped (Fig. 1c). Tiller numbers did not differ significantly between plants grown in Si-supplemented (13.18 ± 0.33) and untreated (12.62 ± 0.35) soil. Root mass decreased in plants subjected to drought, whereas root mass in plants under elevated precipitation did not vary significantly from those under ambient precipitation (Fig. 1d). Root mass was not significantly affected by clipping (clipped: 59.04 ± 2.50, unclipped: 70.18  ± 4.06) or Si-application (supplemented: 59.57 ± 2.97, untreated 64.65 ± 4.06). Full statistical results are shown in Table 1.

Soil and plant chemistry

At the experiment’s conclusion, the Si concentration of Si-supplemented soil was significantly higher than soil that was not supplemented with Si (Fig. 2a), which likely reflected an increase in bioavailable silicon in the soil (c. 21 mg kg⁻¹). The application of Si had no significant effect on the concentration of Si in the roots or shoots of M. stipoides, however. When plants were clipped, the concentrations of Si in the roots and shoots increased by 12 and 41%, respectively, compared with those that were not clipped (Fig. 2b). Grass roots and shoots tended to have higher concentrations of Si (Fig. 2b) and lower concentrations of C (Fig. 2c) under elevated precipitation compared with drought and ambient rainfall conditions, although these effects were less pronounced in the roots (Table 1). Root chemistry, in particular, responded more to drought than elevated precipitation, with Si and C concentrations decreasing and increasing, respectively, under drought compared with ambient rainfall. Concentrations of C and Si were negatively correlated (Fig. 2d). Moreover, Si concentrations in the roots relative to the shoots (i.e. the ratio of root to shoot Si) were significantly reduced in plants under elevated precipitation compared with those under ambient precipitation and drought. In other words, high water availability increased the uptake of Si in plant shoots relative to concentrations in their roots (Table 1). Rainfall had a significant effect on Si uptake efficiency (F_2,30 = 39.83, P < 0.001), which increased by 84% under elevated precipitation (11.87 ± 0.75) and decreased by 40% under drought (3.89 ± 0.35) relative to ambient rainfall conditions (6.46 ± 0.76). In general, average soil moisture content was positively correlated with root (r = 0.25, P = 0.034, df = 70) and shoot Si concentrations (r = 0.61, P < 0.001, df = 70) and negatively correlated with root (r = − 0.26, P = 0.025, df = 70) and shoot C concentrations (r = 0.55, P < 0.001, df = 70). Root N concentrations increased and decreased under elevated precipitation (1.68 ± 0.03%) and drought (1.32 ± 0.03%), respectively, relative to ambient rainfall (1.53 ± 0.04%), whereas shoot N concentrations did not vary between treatments. Rainfall, Si-application and clipping had no significant effects on soil C or N concentrations (Table 1).

Soil and plant responses to Si-application and water availability

The application of Si to the soil increased the retention of water in the soil, especially as rainfall increased, which was likely responsible for the increase in plant height in Si-supplemented plots observed before the clipping treatment was applied. The observed increase in soil water could be associated with either a physical effect of Si-application on the soil profile (via improved soil structure and water penetration; [36]) or an increase in plant water-use efficiency [37]. The application of Si in the form of Na₂SiO₃ could slightly increase soil concentrations of sodium (Na), though treatment concentrations were very low (1 mM) and field soil has the capacity to dissipate impacts. In any case, plants would most likely be unaffected as uptake of Na tends to decrease as plants take up Si [38].

Silicification of leaves has been shown to reduce water loss by as much as 30% in rice [39] and increase the biomass of drought-stressed wheat by 36% [40]. In this case, however, plant shoot and root Si concentrations did not respond to Si-application despite the increase in soil Si concentrations, suggesting that the uptake of plant-available H₄SiO₄ by M. stipoides may already be sufficient in the natural soil environment for optimal growth [41]. Under stressful conditions (e.g. grazing) or high water availability, however, the allocation of Si to structural defence or the passive uptake of Si, respectively, may increase, resulting in an increase in Si above the optimal level for plant growth [42].

Root and shoot Si concentrations and Si uptake efficiency were positively correlated with soil moisture, demonstrating the importance of plant water status in driving Si uptake and storage [13]. The accumulation of Si was associated with decreases in plant C concentrations, a trade-off response that has been identified in other grass species above- [43] and below-ground [10]. More specifically, however, the Si concentration of M. stipoides shoots relative to that of their roots was maintained under drought but was significantly higher under elevated precipitation, which was also evident in plants that had been clipped. The maintenance of shoot Si deposition under drought may be caused by or responsible for the drought tolerance that M. stipoides is generally known for [44], likely associated with osmotic adjustment and nutrient regulation [45]. Other grass species have been shown to maintain [25] or reduce foliar Si uptake under drought [24, 46]. Species-specific differences may be associated with changes in life-history strategies coupled with costs associated with Si accumulation [9]. Alternatively, the benefits of improved drought tolerance associated with Si accumulation in some species may outweigh the relative importance in others [47].

The effects of simulated grazing on plant growth and chemistry

Silicification patterns in roots are understudied, despite evidence that Si concentrations can occur at highest levels in these tissues [11]. This study quantified the short-term changes in Si dynamics in both above-ground and below-ground tissues. In terms of plant growth, the results demonstrated that simulated grazing decreased the number of tillers but had no effect on below-ground biomass. Therefore, no compensatory growth in response to clipping occurred. Instead, M. stipoides responded to clipping by storing Si in its roots and shoots, indicative of an induced defensive response, which was consistent across all watering regimes. Kindomihou et al. [14] also identified a Si-uptake response to simulated grazing in three of five tropical fodder grass species, whereas KM Quigley and TM Anderson [13] identified no effects of simulated grazing on Si accumulation in two Serengeti grasses, suggesting that Si uptake responses are contingent upon plant identity and/or grazing intensity. Grasses that respond to wounding by increasing Si uptake likely involve active processes that are regulated at the gene level, as demonstrated by E McLarnon, S McQueen-Mason, I Lenk and SE Hartley [16]. The effects of simulated grazing on C concentrations showed clear opposite responses to Si concentrations, which were correlated in both roots and shoots and were consistent across all rainfall treatments. Si clearly helps protect grasses from wounding but this investment in structural defence may limit C sequestration.

Simulated herbivory may not mimic natural damage exactly but it enables researchers to control the type, timing and intensity of damage with fewer confounding effects [48], which is especially useful in field systems such as this. Plants subjected to simulated grazing combined with the application of phagostimulants or natural herbivory by grazing vertebrates increase plant defensive responses more than those that are subjected to simulated grazing alone [4]. Moreover, studies with repeated defoliation events (i.e. continuous wounding) have been shown to increase the Si content of grasses compared with single defoliation events, with Si concentrations increasing by up to 400% in response to leaf damage [2] and persisting for several months ([16] and references therein). Microlaena stipoides may therefore show an even greater defence response in the form of higher grazing-induced uptake of Si when subjected to herbivore attack. High levels of rainfall may further enhance Si uptake by increasing transpiration overall [13], with implications for reducing palatability and digestibility by herbivores. Drought, however, is likely to reduce this defensive response to promote resource-conservation (i.e. water and nutrient-uptake) over investment in plant structural defence [49]. Above-ground–below-ground Si distribution only changed under elevated precipitation, under which shoot Si concentrations increased relative to root concentrations. Si may therefore remain in the roots until plants have enough water to enable the transfer of Si from the roots to the shoots.