Judging a salmon by its spots: environmental variation is the primary determinant of spot patterns in Salmo salar
© The Author(s) 2018
Received: 21 August 2017
Accepted: 27 March 2018
Published: 12 April 2018
In fish, morphological colour changes occur from variations in pigment concentrations and in the morphology, density, and distribution of chromatophores in the skin. However, the underlying mechanisms remain unresolved in most species. Here, we describe the first investigation into the genetic and environmental basis of spot pattern development in one of the world’s most studied fishes, the Atlantic salmon. We reared 920 salmon from 64 families of domesticated, F1-hybrid and wild origin in two contrasting environments (Hatchery; tanks for the freshwater stage and sea cages for the marine stage, and River; a natural river for the freshwater stage and tanks for the marine stage). Fish were measured, photographed and spot patterns evaluated.
In the Hatchery experiment, significant but modest differences in spot density were observed among domesticated, F1-hybrid (1.4-fold spottier than domesticated) and wild salmon (1.7-fold spottier than domesticated). A heritability of 6% was calculated for spot density, and a significant QTL on linkage group SSA014 was detected. In the River experiment, significant but modest differences in spot density were also observed among domesticated, F1-hybrid (1.2-fold spottier than domesticated) and wild salmon (1.8-fold spottier than domesticated). Domesticated salmon were sevenfold spottier in the Hatchery vs. River experiment. While different wild populations were used for the two experiments, on average, these were 6.2-fold spottier in the Hatchery vs. River experiment. Fish in the Hatchery experiment displayed scattered to random spot patterns while fish in the River experiment displayed clustered spot patterns.
These data demonstrate that while genetics plays an underlying role, environmental variation represents the primary determinant of spot pattern development in Atlantic salmon.
Animal coats display a fascinating diversity of patterns across different species: the stripes and spots of cats, giraffes and zebras, the symmetry and colour of butterfly wings, and exotic swirl patterns on shellfish and fish . Morphological colour changes in fish occur from variations in pigment concentrations and in the morphology, density, and distribution of chromatophores in three dimensions in the integument of the skin. Despite the wide variety of colours and patterns observed, the structural organization of pigments in teleost fish display similar features, suggesting a common mechanism of development and colour revelation .
All vertebrate chromatophores originate from the neural crest in the embryo, formed shortly after gastrulation . The birth, migration, and differentiation of chromatophores is tightly regulated by signalling pathways and transcription factors [2, 3]. Although at least 100 genes involved in patterning in vertebrates have been identified, little is known about the molecular or cellular processes that generate complex patterns, or how alterations in those processes might produce different phenotypes . Recent experimental studies [4–6], notably also in zebrafish (Danio rerio), point to the mechanism being Turing pattern formation, acting through differences in diffusion or cell–cell communication and reaction across the fish skin surface [7–9].
The pigment patterns of wild animals, including fish, are of importance to their fitness and serve a variety of functions in predator avoidance, prey capture and social communication [2, 3]. Melanin-based colouration may co-vary with traits such as dominance, stronger immune responses, and greater stress resistance . Present evidence also suggests that fitter, dominant fish are spottier . Pigment phenotype, or “spottiness” as it is commonly known, is often determined by polymorphisms in the melanocortin-1 receptor (MC1R) in vertebrates, and this receptor family is also involved in regulating immunity and stress responses [3, 10]. The specific role of melanophores is to protect the skin from UV damage . In anadromous fish, such as Atlantic salmon (Salmo salar L.), plasticity of patterning is probably the consequence of ontogenic habitat shifts . Patterning is a sequential process where the coat markings (known as ultimate patterns) repeatedly change as a result of a series of transitions between life stages such as parr/smolt and immature/nuptial . At the present, only general information is available about skin colours and patterns in salmonids, and their genetic basis and environmental triggers are largely unknown . One exception is the fine-spotted pigmentation pattern observed in some populations of brown trout (Salmo trutta L.) which has been shown to be inherited in a simple Mendelian pattern [14, 15]. Quantitative trait loci (QTL) analysis can assist in understanding the relationship between inheritance and phenotype and is an early step in identifying key genes . Life-stage skin colouration can also be modulated in response to environmental factors, allowing fine-tuning of the ultimate patterns. Environmental factors can be primary when they have a direct effect on chromatophores, or they can be secondary, allowing further adjustment of colour during a life stage .
Atlantic salmon are characterized by a wide variety of genetic-based life-history, phenotypic and morphological diversity within and among populations . This species has also been subject to domestication selection since the early 1970s , and as a result, domesticated salmon display a wide variety of genetic differences to wild salmon . Notably, growth rate under controlled farming conditions is now several-fold higher than for wild salmon [20, 21]. In addition, variation in spot patterns between domesticated and wild salmon have been reported, with domesticated salmon displaying more spots [22, 23]. However, whether the difference in spot patterns between domesticated and wild salmon results from environmental or genetic variation remains to be determined. Salmon are easy to rear, and common-garden experiments can be conducted in a variety of environments ranging from farms [21, 24] to natural river systems [25, 26]. Such studies provide the ability to disentangle the relative roles of genetics, environment, and developmental triggers for phenotypic traits, such as spot patterns. In the present study, we aimed to determine whether the reported difference in spot patterns between domesticated and wild salmon is caused by genetic or environmental variation. In order to address this, we reared pedigree-controlled domestic, F1 wild-domesticated hybrid and wild salmon in both a natural river environment, as well as a hatchery, and quantified their spot patterns using a mixture of manual and automatic spot counting methods.
Overall study design
Production of the fish for the Hatchery experiment
Production of the fish for the River experiment
Eyed eggs of domesticated, wild and F1 hybrid salmon families were planted into the river Guddal (61°14′N, 5°37′E) in 2008 and 2010 (Fig. 2). The planted families were established from broodfish captured in the river Etne (59°40′N, 5°56′E), as well as the domesticated Mowi strain, and their F1 hybrids. Eyed-eggs from 17 families (2–8 families per cross, family range Q1-30) and 29 families (9–10 families per cross, family range Q31-60) were planted in the river in April 2008 and 2010, respectively (Fig. 2; Additional file 1). This natural river system has previously been used for common-garden experiments involving planting out genetically diverse fish that are subsequently identified by DNA parentage testing [25, 28]. The planted eggs hatched naturally, and juveniles competed for resources until they smoltified at age 2+ and 4+. The river Guddal is a summer-cold river as it receives run-off from the Folgefonna glacier, limiting growth rates. Average survival from eyed egg to the smolt stage in this river is typically 1–2% based upon previous experiments . Upon smoltification in May 2012, we caught a random sample (initial n = 379 consisting of 4 year smolts representing 16 families from cohort 2008 and 2 year smolts representing 9 families from cohort 2010) of the surviving fish planted into the river in a downstream trap, and thereafter transported them to the Matre fish farm where they were placed into 1.5 m2 rearing tanks with saltwater. At Matre, these fish were weaned onto Skretting pellets. These fish were reared in saltwater tanks from May 2012 onwards, fin clipped and PIT tagged in March 2013 and phenotyped for this study in April 2014 at the age of 4+ and 6+. In the river, salmon were exposed to natural light and had access to natural hiding places with shade. After being transferred to Matre, the salmon were held in tanks experiencing the natural day-length for Bergen. Tank colour was white for the first year (May 2012 to February 2013), and green during the second year. Osram L18W/865 Lumilux Cool Daylight, 1300 lm (UV free light) were used as the light source in fish tanks.
Phenotyping fish from both experiments
In April 2014, salmon from the Hatchery (n = 2249) and River (n = 344) experiments were sampled. Fish were first anaesthetized with metacain (Finquel® Vet, ScanVacc, Årnes, Norway), thereafter identified by their PIT tag, measured, and then photographed using a digital Canon EOS 650D camera. DNA parentage testing with microsatellites was used to identify all fish to their families and thus strains/populations of origin. Extensive details with respect to the exact genotyping procedure are available elsewhere .
From the Hatchery experiment, up to 20 individuals per family were selected for the image analysis (Fig. 1) using random selection in R version 3.2.2 . In the River experiment, we used the same criteria for selection of individuals as above, however, many families were represented with a low number of individuals (reflecting differences in family survival in the river as has been observed previously ). Among the fish randomly chosen for image analysis from the Hatchery experiment, early maturing fish (n = 33) were excluded from the analysis. Early maturation was not registered for fish from the River experiment. Thus, the total number of individuals used for image analysis were 745 and 185 for the fish originating from the Hatchery and River experiments respectively. All raw data are presented (Additional file 2).
Quantification of spots
In addition to automatic spot counts, human judgement was used to assess the overall spottiness of the fish. This counting method mirrored the results of the automatic method, and therefore, these data are not presented. The number of spots on the fishes’ head between the gills and the crease behind the gills, and from the crease to the eye of the fish were counted manually (Fig. 3, top). The entire head of the fish was not always represented in the photograph, and consequently, there were missing values: Hatchery (n = 4), River (n = 54).
All salmon from the Hatchery experiment were subject to linkage mapping using an identical approach to that described in . In short, this included analysis of genome-wide distributed SNPs and associating their genetic variation to measurements of spottiness. An initial set of 118 SNP markers (Additional file 4) were selected as good candidates to cover the salmon genome at regular 20–30 cM intervals based on the salmon linkage map , and for having provided the best information content in a similar experimental design . After genotyping was performed on a MassARRAY Analyzer 4 (Agena Bioscience™), a few of the SNPs did not amplify. This left the final number of SNPs at 109. The raw data was processed in Typer 4.0.20. The phenotypes used for the QTL analysis are found in Additional file 5, and the genotypes in Additional file 6. Genes in the detected linkage group can be found in Additional file 7. Genes identified by KEGG as participants in melanosome biogenesis are found in Additional file 8.
To perform QTL mapping, we first reconstructed the haplotypes of both parents and offspring based on pedigree and genotype data. The next step consists in estimating the Identity By Descent (IBD) coefficient between each pair of individuals at each locus along the genome. IBD coefficients were obtained from a recursive approach  implemented to account for haplotype information as input.
In both models, we consider the adjusted profile log-likelihood profiled over random effects as provided by HGLM [30, 36]. The likelihood ratio between model 1 and model 2 is then considered as the indicator for QTL i.e., correlation between genotype and phenotype variance.
To account for multiple testing along the genome, the genome wide significance threshold for likelihood ratio was obtained through a permutation test as in Churchill and Doerge .
All data processing was carried out in R version 3.2.2 . Correlation between response and explanatory variables was estimated using anova and linear models (not indicated in main text when used). To estimate the variance components of spot density, a mixed model was fitted using the lmer() function from the lme4 package . A Kruskal–Wallis rank sum test was employed when testing the effect of strain on categorical data (spottiness, spots below lateral). Mann–Whitney (for categorical data) and T tests (for numerical data) were used for direct comparisons between the Hatchery and River experiments, and between the domesticated populations (Mowi) in the Hatchery and River experiments. Fish with no spots were excluded [missing in the Hatchery experiment n = 6 (4 Mowi, 2 Arna), River experiment n = 3 (all Mowi)] from analysis of automatic count variables (ROI size—and therefore all spot densities corrected for this), since these depend on the presence of spots. In the analysis of ANN values, 10 spots were chosen as an arbitrary threshold for exclusion, since whether the spots are near or far apart is random at low spot numbers [fish excluded in the Hatchery experiment n = 37 (9 Mowi, 2 F × M, 4 M × F, 11 Arna, 6 Figgjo, 5 Vosso)], River experiment [n = 24 (15 Mowi, 4 M × E, 5 Etne)]. In the analysis of influence of family on spot density, families with only one member were removed in the River experiment [n = 4, Q49 (Mowi), Q34, Q40, Q50 (M × E)]. The box plots used for data display are R default.
Spot counts, fish weight and spot density
Number of spots (automatic counts within region of interest (ROI)) and weight (g) per strain
No. of spots (n per ROI)
Figgjo × Mowi
Mowi × Figgjo
Mowi × Etne
In both experiments, the number of spots correlated with fish size (Additional file 9: Figure A9.1) [R2 = 0.19, p < 2.2e−16 (Hatchery) and R2 = 0.07, p = 0.005 (River)]. Furthermore, fish weight and rectangular window size used to count the number of spots were strongly correlated (Hatchery, R2 = 0.94 p ≤ 2.2e−16, River, R2 = 0.94 p < 2.2e−16, Additional file 9: Figure A9.2). Therefore, to exclude the potentially biasing influence of fish size on estimates of spottiness, we adjusted the head spot counts for fish size using weight, and we adjusted the automatic spot counts using window size (ROI area), resulting in measures of spot density.
Genetic basis of spot density
Head spot counts and spots below the lateral line
Fish from the Hatchery experiment displayed an average of 10–15 head spots (13.3 ± 6.9), while fish from the River experiment displayed an average of six head spots (6.0 ± 3.1) (t test Hatchery expt. Vs. River expt. 2, t = 19.1, df = 389.5, p < 2.2e−16) (Additional file 9: Figure A9.3). Adjusted for fish weight, the trends per strain in the data also resemble those for spot density of the whole fish (Additional file 9: Figure A9.4 compared to Fig. 5). Head spot density and overall spot density were correlated in both datasets [Additional file 9: Figure A9.5, (a) Hatchery, R2 = 0.39 p < 2.2e−16. (b) River, R2 = 0.31, p = 3.6e−14]. Counts of spots below the lateral line were highly correlated with total spottiness in both experiments (Hatchery, R2 = 0.86 p < 2.2e−16. River, R2 = 0.92 p < 2.2e−16), Additional file 9: Figures A9.6, A9.7).
This study provides the first experimental insight into the primary determinant of spotting patterns in one of the world’s most extensively studied fishes, the Atlantic salmon. We observed heritable variation and a significant QTL for spot density, demonstrating an underlying genetic influence for this trait. However, environmental variation was clearly the primary determinant as fish of domesticated, wild-domesticated F1 hybrid and wild pedigree all displayed a six to sevenfold higher density of spots when reared under farming conditions (Hatchery experiment—fish tanks, then sea cages), as opposed to a combination of the river and thereafter farming conditions (River experiment—natural river, then fish tanks). Different wild populations were used in the two experiments, potentially confounding the comparisons between environments. However, the domesticated Mowi strain was used in both experiments and showed a sevenfold higher density of spots in the Hatchery vs. the River experiment. We therefore conclude that environmental variation, as opposed to genetic variation, is the primary determinant of spot patterns in Atlantic salmon.
Our results beg two questions: what are the causative environmental triggers, and when are the fish receptive to these triggers? The available evidence from our study indicates that the diversity in spot patterns observed here were primarily set in the freshwater stage. Evidence supporting this includes: (1) Despite being reared in tanks for the marine phase, the fish reared in the natural river system up to smoltification (i.e. the River experiment) retained a clustered spot pattern with low spot density like that typically observed in salmon spending their entire lives in the wild [23, 39], (2) Kause et al. , reported no influence of freshwater vs saltwater-rearing on spot patterns in rainbow trout (Oncorhyncus mykiss) that were produced under identical rearing conditions until smoltification, (3) data from salmonids generally demonstrate that spot patterns are largely persistent over time, albeit with modifications [41–43]. Nevertheless, evidence suggesting that spot patterns can change during the marine phase can be obtained from a study of morphological differences between fish from farms and the wild . These authors reported that salmon originating from six different farms displayed a greater number of spots than salmon originating from several rivers, a result consistent with ours. In contrast to the present study, however, these authors reported that salmon reared in tanks up to and including smoltification, then released into the sea to complete their marine phase of the life cycle in the wild, displayed spot patterns similar to wild salmon. In the present study, the fish reared under farming conditions were kept in sea cages post smoltification while the fish initially starting their life’s in the natural river system were held in tanks on land during the marine stage. This means that the exact environmental trigger that leads to spot pattern development cannot be determined from present evidence. Nevertheless, our data unequivocally demonstrate, for the first time, that environmental, as opposed to genetic variation, is the primary trigger for spotting patterns in this globally significant species.
Any combination of the environmental differences between freshwater rearing in the river and then marine rearing in tanks, vs. freshwater rearing in tanks and then marine rearing in open sea cages, may have triggered the diversity in spot patterns observed here. These could include, among other factors, light, diet, predation (selection), temperature, habitat, fish density and growth rate. Light is known to influence spot patterns in several ways . 24-hour artificial lighting (typically lacking UV), is standard in salmon farming (including our study), in contrast to the situation in rivers. However, different artificial light regimes are not known to cause differences in spot patterns. Diet can affect melanisation . Different farms and rivers have different dietary conditions, and current evidence suggests that the patterns form predictably regardless of this . Salmon with many spots have been found to be less stress responsive and more dominant , and it is possible that this plays a role in the differences in pattern formation between the farm and the wild. However, a 14-week stress experiment in a hatchery did not result in pattern differences noticeable by eye . The controlled conditions of fish farming are very well suited for further studies aiming to identify the trigger signal in Atlantic salmon.
With notable exceptions, current understanding of the genetics of spot pattern development in salmonids in general is sparse. Skaala and Jørstad demonstrated that the difference between fine and normally spotted brown trout is caused by a single unidentified gene displaying Mendelian inheritance . Sivka et al. found that differences in spot patterns between brown trout and marble trout (Salmo marmoratus) is linked to differences in the expression of four genes involved in Wnt signalling, melanosome biogenesis and proopio-melanocortin (POMC) processing . We found that narrow sense heritability was low for number of spots (14%) and spot density (6%), much lower than previously found for silver and spotty character in rainbow trout (23–45%) [13, 40]. Furthermore, we observed a significant QTL associated with spot density on linkage group SSA014. MC1R, a well-known determinant of degree of spotting in vertebrates , lies on linkage group SSA011 in Atlantic salmon. Thus, in our study, we did not observe a link between MC1R and the observed genetic variation. The linkage group covers over 4000 transcripts, but different isoforms of adenylate cyclase (ADCY1, ADCY2, ADCY8), endothelin B receptor (EDNRB) and MEK2 (MAP2K2) have predicted sequences within it, all of which are known participants in melanogenesis according to KEGG. Colouration has been shown to be regulated irrespective of both the sequence and expression level of MC1R in other poikilotherms . Furthermore, in mammals, mutations in EDNRB causes a complete lack of spots, but in zebrafish this has been shown to only halve the number, which seems consistent with the effect seen here . Thus, EDNRB is a good candidate for explaining our QTL hit.
Our results have an important application. Domestication of Atlantic salmon was first initiated in Norway in the early 1970s. As a result, domesticated salmon display genetic differences to wild salmon in a wide range of traits . Each year, large numbers of domesticated Atlantic salmon escape from farms, and many find their way onto the spawning grounds of native populations. As a result, introgression of domesticated salmon has occurred in a large number of wild salmon populations [45, 46], and a reduction in population-genetic differentiation has been documented in Norway where this has been studied . Therefore, identification, and subsequent removal of escapees from rivers is of importance to protect native populations from further impact. On the river bank, escapees are typically identified through deviating morphological characteristics such as fin erosion, spottiness and body condition [22, 23]. However, some of the morphological characteristics used for identification are plastic, and may partially or completely revert to the wild-type when they have been in the wild for an extended period of months or possibly years. Here, we provide further evidence that spot patterns, while primarily environmentally-induced, can be used to assist sorting farmed and wild fish in the rivers.
KAG, MFS conceived and designed the study. ØS produced the fish in Guddal. MFS, PGF phenotyped the fish. AT, KJ established the automatic spot counting software. KJ, KM, FB analysed the data. KJ wrote the first draft of the paper and all authors read and contributed to the paper. KAG managed the study. All authors read and approved the final manuscript.
This study took over 7 years to produce the fish, and involved field, hatchery and laboratory logistics. We would therefore like to acknowledge the generous efforts of a large number of technical staff at IMR for assistance in various parts of the process. We are especially grateful to Lise Dyrhovden and Ivar Helge Matre for hatchery assistance, and Anne-Grete Eide Sørvik and Geir Dahle for genotyping assistance. Finally, we gratefully acknowledge the river Guddal land-owners organisation for collaboration in the river. Thanks also to Karen Gjertsen, NMD, for producing Fig. 2.
The authors declare that they have no competing interests.
Availability of data and materials
All data generated or analyzed during this study are included in this published article: Additional file 2: Raw data, Additional file 4: SNPs for QTL analysis, Additional file 5: Phenotypes for QTL data, Additional file 6: Genotypes for QTL data.
Consent for publication
Ethics approval and consent to participate
The experimental protocols (Permit Number 5296 for the Hatchery experiment, and 5186 for the River experiment) were approved by the Norwegian Animal Research Authority (NARA). Welfare and use of experimental animals was performed in strict accordance with the Norwegian Animal Welfare Act of 19th of June 2009, in force on the 1st of January 2010, while all personnel involved in the experiment had undergone mandatory training approved by the Norwegian Food Safety Authority.
The study was funded by two grants from the Research Council of Norway. The Hatchery experiment, Interact (No. 200501) and the River experiment, QuantEscape (No.216105).
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