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Palaeoenvironmental drivers of vertebrate community composition in the Belly River Group (Campanian) of Alberta, Canada, with implications for dinosaur biogeography

BMC Ecology volume 16, Article number: 52 (2016) Cite this article

  • The Erratum to this article has been published in BMC Ecology 2017 17:1

Abstract

Background

The Belly River Group of southern Alberta is one of the best-sampled Late Cretaceous terrestrial faunal assemblages in the world. This system provides a high-resolution biostratigraphic record of terrestrial vertebrate diversity and faunal turnover, and it has considerable potential to be a model system for testing hypotheses of dinosaur palaeoecological dynamics, including important aspects of palaeoecommunity structure, trophic interactions, and responses to environmental change. Vertebrate fossil microsites (assemblages of small bones and teeth concentrated together over a relatively short time and thought to be representative of community composition) offer an unparalleled dataset to better test these hypotheses by ameliorating problems of sample size, geography, and chronostratigraphic control that hamper other palaeoecological analyses. Here, we assembled a comprehensive relative abundance dataset of microsites sampled from the entire Belly River Group and performed a series of analyses to test the influence of environmental factors on site and taxon clustering, and assess the stability of faunal community composition both temporally and spatially. We also test the idea that populations of large dinosaur taxa were particularly sensitive to small-scale environmental gradients, such as the paralic (coastal) to alluvial (inland) regimes present within the time-equivalent depositional basin of the upper Oldman and lower Dinosaur Park Formations.

Results

Palaeoenvironment (i.e. reconstructed environmental conditions, related to relative amount of alluvial, fluvial, and coastal influence in associated sedimentary strata) was found to be strongly associated with clustering of sites by relative-abundance faunal assemblages, particularly in relation to changes in faunal assemblage composition and marine-terrestrial environmental transitions. Palaeogeography/palaeolandscape were moderately associated to site relative abundance assemblage clustering, with depositional setting and time (i.e. vertical position within stratigraphic unit) more weakly associated. Interestingly, while vertebrate relative abundance assemblages as a whole were strongly correlated with these marine-terrestrial transitions, the dinosaur communities do not appear to be particularly sensitive to them.

Conclusions

This analysis confirms that depositional setting (i.e. the sediment type/sorting and associated characteristics) has little effect on faunal assemblage composition, in contrast to the effect of changes in the broader palaeoenvironment (e.g. upper vs. lower coastal plain, etc.), with marine-terrestrial transitions driving temporal faunal dynamics within the Belly River Group. The similarity of the dinosaur faunal assemblages between the time-equivalent portions of the Dinosaur Park Formation and Oldman Formation suggests that either these palaeoenvironments are more similar than characterized in the literature, or that the dinosaurs are less sensitive to variation in palaeoenvironment than has often been suggested. A lack of sensitivity to subtle environmental gradients casts doubt on these forces acting as a driver of altitudinal zonation of dinosaur communities in the Late Cretaceous of North America.

Background

Differences in faunal composition in the Late Cretaceous of Western North America have been hypothesized to reflect adaptation to latitudinal and altitudinal climatic gradients [1–7]. Environmental changes caused by transgression-regression cycles of the Western Interior Sea have been suggested to drive the high diversity and high faunal turnover rates of non-avian dinosaurs [4, 5, 8–11], along with changes in the vertebrate community structure more generally [12–16]. However, global-scale analyses of dinosaurs, as a whole [17] and at the family level [18], indicate that large-scale changes in sea level may not have had a significant influence on broad patterns of diversity, evolution, or migration. This suggests that putative patterns in dinosaur ecology and evolution related to sea level, such as those described from Western North America, may be either the result of other factors, such as sampling biases, or may be occurring on a scale that is too small to be readily detected in such coarse-scale analyses [4]. Previous studies have suggested that the composition and diversity of taxa recovered from specific fossil localities across Western North America varies depending on their distance from the palaeoshoreline of the Western Interior Sea [1–3, 5, 7, 19, 20]. This has led to considerable debate regarding the degree of provinciality/endemism in dinosaur populations [1–7, 9, 10, 14, 15, 19–28], the putatively high diversity and restricted range of dinosaur taxa when compared to modern large mammals [2, 5, 7, 19, 20, 22, 29–37], as well as discussions of niche-partitioning in dinosaurs across environmental gradients in a single depositional basin [2, 5, 10, 19, 20, 23, 24, 29, 30, 38–45]. These noted variations in palaeocommunities over sub-million year timeframes and over relatively small palaeogeographic areas suggests that many taxa, but particularly large-bodied dinosaurs, may have been sensitive to palaeoclimatic and palaeoenvironmental change [1–3, 5, 7, 9, 43, 44, 46]. However, this model has been challenged for reliance on data derived from disjunct geographic areas that are poorly constrained chronostratigraphically [28]. the ability to test hypotheses about dinosaur biogeography, endemism, and environmental sensitivity has historically been difficult, as many specimens were collected with only limited geological data or stratigraphic information, and known by very low sample sizes [24, 43, 47], though ongoing work collecting new specimens, relocating these sites, and incorporating them into the broader stratigraphy is ameliorating some of these issues [23, 24, 43, 44, 48].

The Belly River Group of southern Alberta is one of the best-sampled Late Cretaceous vertebrate fossil deposits in the world [44], providing a high-resolution biostratigraphic record of terrestrial vertebrate diversity and faunal turnover, and has considerable potential to be as a model system for testing hypotheses of dinosaur palaeoecological dynamics, including important aspects of palaeoecommunity structure, trophic interactions, and responses to environmental change [48]. The Belly River Group is composed of the Foremost, Oldman, and Dinosaur Park formations, and spans a large portion of the Campanian from a period of time from approximately 79–74 Ma [1, 49, 50]. The full extent of the Belly River Group records two major regional sea level changes in the Western Interior Seaway (the relatively shallow, inland seaway that at its greatest extent stretched from the Arctic Ocean to the Gulf of Mexico), the first of which is a regressive event in the Foremost and lower Oldman formations, and the second of which is a major transgressive event recorded in the uppermost Oldman and Dinosaur Park formations that marks the transition between the Belly River Group and the overlying Bearpaw Formation [49, 50]. The Foremost Formation is the stratigraphically lowest unit within the Belly River Group, and conformably overlies the marine shales of the Pakowki Formation. The earliest Foremost sediments show considerable marine influence, and the formation, going from lowest to highest outcrops, is generally composed of paralic to non-marine sediments, following a coarsening upwards succession [50, 51]. Conformably overlying the Foremost Formation is the Oldman Formation. The Oldman Formation is broadly considered to represent more fluvial, inland conditions, and is made up of series of upward fining palaeochannel sandstone successions, with a variety of channel top, channel margin, and overbank facies [12, 14, 50, 52]. The amount of exposure of upper Oldman sediments is dependent on sampling location, with southern sites showing a thick succession of strata, and northern sites truncated above the middle Oldman by a regional disconformity [14, 50]. To the north, upper Oldman Formation strata are replaced disconformably by those of the Dinosaur Park Formation as a result of clastic-wedge replacement [50]. The Dinosaur Park Formation (DPF) is considered to have a greater coastal influence than the underlying Oldman Formation (OM), and is characterized by sandy to muddy, alluvial, estuarine, and paralic facies [12, 49, 50, 52]. The transition to more marine environment in the upper Dinosaur Park Formation is marked by the deposition of the Lethbridge Coal Zone (LCZ), which interfingers with and is overlain by marine shales of the Bearpaw Formation [13, 49, 53]. The pre-LCZ section of the Dinosaur Park Formation in Dinosaur Provincial Park (DPP) is thought to be broadly time-equivalent to the upper Oldman Formation in the Milk River/Manyberries (MRM) regions of southern Alberta [14, 50]. The muddy strata of the upper Oldman Formation in Milk River/Manyberries are typically thought to be more environmentally similar to that of the middle Oldman ‘Comrey sandstone’ (representing a more seasonally arid, inland, fully non-marine fluvial landscape) than to the time-equivalent pre-LCZ Dinosaur Park Formation in DPP (representing a wetter, more marine-influenced coastal plain) [14, 49, 50]. This view has been questioned by other studies suggesting the time-equivalent Oldman and Dinosaur Park formations share a generally wet, coastal environment and that variations in recorded wet-dry signal may represent seasonality and/or spatial variation in local habitat [22, 54]. Interpreting these units is complicated by the existence of heterogeneity in their geographic extent and palaeoenvironment through time, particularly in their relation to the shore of the Western Interior Seaway [5, 8, 19, 20, 24]. Meaningful comparison between these units requires detailed chronostratigraphic control to mitigate the confounding effects of temporal changes in community structure. This is particularly important because the distribution of dinosaurs has been hypothesized to be sensitive to even small-scale palaeoenvironment differences, such as those between the lower and upper coastal plain settings of the time-equivalent Oldman and Dinosaur Park formations [5, 14, 24, 50, 52].

Vertebrate microfossil sites from the Belly River Group have provided a wealth of knowledge on vertebrate palaeoecology [12, 14, 15, 52, 55, 56]. Vertebrate microfossil sites, sensu [57], are useful in overcoming issues of low sample size that commonly hinder palaeontological investigations, as they are both abundant and each site can preserve large numbers of small teeth, bones, and scales of numerous taxa thought to represent much of the vertebrate community composition of a given area [12–16, 53, 55, 58]. These sites are concentrated in a number of ways, with most representing in-channel deposits, crevasse splays, low energy ponds, or shoreface lag deposits [14, 16, 52, 59, 60]. While vertebrate microfossil sites in a given area or formation may represent the same broader palaeoenvironment (e.g. upland fluvial system, lowland coastal plain, etc.), their method of deposition may differ (e.g. flow rates, depositional energy, sediment size/sorting, etc.), and this has led to concerns that microsites with differing depositional setting may not preserve comparable vertebrate material or faunal assemblages [14, 59]. These concerns regarding the effect of depositional setting on the presence/absence and ranked abundances of taxa at a given microvertebrate site have been partially lessened by a new taphonomic model for microsite formation suggesting that depositional setting may not play a large role when comparing sites of different type, as channel deposits may represent the short-term erosion and local re-deposition of lower-energy pond deposits [59]. Microsite studies in the Belly River Group of Alberta have so far focused on identifying the taxa present across the region, and assessing any broad trends or associations that may be present through specific stratigraphic intervals [8, 12–14, 16, 53, 58, 61]. These studies have identified aquatic and terrestrial communities, along with some evidence of potential endemism. Additionally, they have demonstrated that terrestrial-marine environmental transitions drive at least some of the changes in vertebrate faunal assemblages, such as an increase in ceratopsid dinosaur abundance correlated with increasing marine influence in the Dinosaur Park Formation of DPP [8], and an inverse relationship between sharks and lissamphibian abundances in the Foremost Formation [16]. However, major quantitative studies have focused on either the transgressive or regressive sequences, and not the entirety of the Belly River Group, resulting in our understanding of the environmental drivers behind microsite faunal assemblage structure remaining incomplete despite the abundance and quality of the available data. In addition, new data suggests that dinosaur species found in the Dinosaur Park Formation of DPP are also found in the time-equivalent sections of the Oldman Formation in MRM [22, 24]. These units have been described as representing different palaeoenvironmental regimes [14, 22, 24, 50]. However, the Oldman Formation is palaeoenvironmentally dynamic throughout its history, shifting from lowland coastal plain to more inland braided rivers, and back to lowland coastal plain [14, 16, 22, 50]. As a result, comparisons of vertebrate microfossil sites can provide an important test of habitat sensitivity in dinosaurs.

Given the ongoing debate regarding the putatively narrow associations of dinosaurs with particular environments, locations, and/or geological formations, this study uses the largest Cretaceous vertebrate microsite dataset yet assembled to first test the previously suggested associations between faunal assemblages and differing environments, and then use those as a proxy to test for differences in dinosaur assemblages in the time-equivalent sections of the Dinosaur Park and Oldman formations. We hypothesize that altitudinal (inland vs. coastal) effects will act as the largest driver of faunal assemblage change, following previous results on more limited datasets, with other taphonomic or temporal effects acting minimally on the preserved ecological signal. We also hypothesize that dinosaurs, particularly those of large body size, will not be sensitive to altitudinal change as recorded in the Belly River Group. Although it is frequently hypothesized that large dinosaurs are sensitive to environmental variation [1–9, 11, 14, 15, 19–24, 26, 27, 42, 43, 62], many groups of large mammals today are resilient to environmental variation and have broad latitudinal distributions [2, 7, 32].

Methods

Dataset integration and taxonomic consistency

A dataset (Table 1) of vertebrate microfossil taxon abundance for Belly River Group sites (N = 48) was created through the merger of multiple literature sources [12–14, 16, 53, 58, 61]. These sites span the duration of the Belly River Group (BRG), and were sampled spatially from Dinosaur Provincial Park (DPP) and from the area around the Milk River south of Manyberries (MRM) (Fig. 1). Some revision to the taxonomic categories was required in order to successfully merge these datasets, with specific changes related to differences in the inclusivity of taxonomic categories in each source dataset (e.g. ‘mammals’ vs. genus-level assignment of respective taxa, due to a lack of genus resolution in included DPP data; inclusion of specimens of Allocaudata within Caudata in early studies and separation in later studies, Brinkman pers. comm. 2016), updating terminology to reflect more recent taxonomic revisions (e.g. Atractosteus to Lepisosteus for BRG gars, ‘teleost D’ to Coriops), noting areas of potential future taxonomic revision (e.g. Myledaphus to Myledaphus + Pseudomyledaphus, as the latter may exist in multiple sites under the former name), and revisions to identifications based on discussions with the collectors of the source microsite data (e.g. Pachycephalosaur material being now referred to hypsilophodont, Brinkman pers. comm. 2016) [14, 16, 63, 64].

Table 1 Taxon abundance table for each site analyzed in this study, with information derived and standardized from literature [12–14, 16, 53, 58, 61]
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Fig. 1
figure1

Locality map of Belly River Group sites analyzed in this study. a Geographic location of sites in Alberta, in context to regional landmarks; b relative stratigraphic positions of sites within the Belly River Group at each sampling region. Map modified from [16] and site locality data compiled from [12, 14, 16]

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Dataset standardization and R- vs. Q-mode cluster analysis

The combined data matrix was rarefied in R using functions contained within the ‘vegan’ package [65]. This was done to compare sampling intensity between sites, and found that few sample issues exist between sites, though most sites are likely somewhat undersampled compared to their theoretical optimum. Rarefied data were further Wisconsin double standardized after conversion to relative abundances, again through use of the functions contained in the ‘vegan’ package [65]. The standardized relative abundance dataset was converted to the percentage-difference dissimilarity index (also referred to as Bray-Curtis) [66]. R- vs. Q-mode cluster analyses were generated for the resultant dissimilarity dataset, with UPGMA linkage, using the ‘tabasco’ function of the ‘vegan’ R package [65]. DPP and Milk River sites were identified on the resulting plots, and major site/taxon clusters were highlighted.

The R- vs. Q-mode cluster analysis was repeated for three sub-sample analyses focused on the time-equivalent upper Oldman and Dinosaur Park formations, one including only dinosaur proportions, another including only theropod proportions, and a third including proportions of all non-dinosaurs shared between these sites. The first two subsamples contained different source data (Additional file 1) for Dinosaur Provincial Park sites than the comparisons of total vertebrate assemblages, being derived from the re-sampled dinosaur material of Brinkman et al. [8] instead of Brinkman [12]. The dataset of Brinkman et al. [8] allows for more detailed taxonomic comparisons when restricted to dinosaur data (due to the inclusion of several additional small theropod genera), but is not considered appropriate for the broader vertebrate faunal comparisons due to the lack of methodological consistency (associated with the targeted nature of this additional dinosaur sampling) relative to the faunal data for other vertebrate material in Dinosaur Provincial Park sites. The non-dinosaur subsample used the same source data as the primary analysis of all vertebrates, the only difference being the removal of dinosaurs from the dataset. This last analysis was performed to confirm the effect of dinosaurs on overall trends in the data, and avoid possible issues of circularity in interpreting the dinosaur data in isolation.

Environmental factors and redundancy analysis

Data relating to the palaeoenvironmental setting (marine, transitional, terrestrial—paralic/lower coastal plain, terrestrial—alluvial/upper coastal plain), palaeogeography (Dinosaur Provincial Park localities, Milk River localities), stratigraphic interval (Foremost Formation, lower Oldman Formation, middle Oldman Formation or ‘Comrey sandstone’, time-equivalent upper Oldman and pre-LCZ Dinosaur Park formations, Lethbridge Coal Zone of Dinosaur Park Formation), and depositional setting (shoreface deposit, crevasse splay deposit, in-channel deposit) were taken from the literature [12–14, 16, 52, 53, 58, 61] and assembled into an environmental data matrix (Additional file 2). Though the palaeogeographical separation between the sampling localities is relatively small in the context of latitudinal climate gradients (~150 km apart, with Dinosaur Provincial Park at ~50.75° latitude and Milk River at ~49.15° latitude), it is of a similar magnitude to previous analyses of vertebrate endemism in microsites [8, 14–16, 53, 58] and endemism/provinciality of large dinosaur macrofossils [1, 5, 19, 20, 37, 43, 44]. The environmental matrix and the dissimilarity matrix generated for the cluster analyses were then ordinated via redundancy analysis (RDA) in order to assess the relationship between each environmental variable and the clustering of site faunal assemblages.

Pair-wise site assemblage similarity

Relative abundance data of site faunal assemblages were split into two smaller datasets corresponding to sampling location (DPP vs. MRM), and ordered stratigraphically. The proportions of each taxonomic group were then plotted and compared using the R packages ‘ggplot2’ and ‘reshape2’ [67, 68]. Additionally, pair-wise Bray-Curtis similarity values were computed for sites from each sampling area using the ‘fossil’ package [69], and plotted as curves showing relative changes in site similarity through stratigraphy. Average faunal proportions for each stratigraphic interval were also produced and plotted.

This was repeated in three sub-sampling analyses focused on the time-equivalent upper Oldman and Dinosaur Park formations, one including only dinosaur proportions, another including only theropod proportions, and one including non-dinosaur proportions. As with the R- vs. Q-mode subsamples, the first two sub-sample analyses used the re-sampled dataset of Dinosaur Provincial Park sites (from Brinkman et al. [8]), for the same purpose of more specific dinosaur taxon comparability, while the third sub-sample used the primary dataset (with dinosaurs removed).

Results

R- vs. Q-mode cluster analysis

Cluster analyses of sites (Q-mode) and taxa (R-mode) were performed and compared to identify major clustering trends among Belly River Group microsites (Fig. 2). Two primary site clusters were identified, with an additional grade of sites between them. The largest site cluster (yellow highlighted component in Fig. 2) contains all Oldman Formation sites (N = 23), along with a majority of the pre-LCZ Dinosaur Park Formation sites (N = 14, out of a possible 18), and one Foremost Formation site (‘SPS’). This cluster contains two large sub-clusters, which broadly group sites based on their sampling region (either DPP or MRM). The second primary site cluster (blue highlighted component in Fig. 2) contains the three stratigraphically lowest Foremost Formation sites (‘PHR-1’, ‘PHR-2’, ‘PHRN’) and both Lethbridge Coal Zone sites (‘BB96’, ‘L2377’). The grade of sites (green highlighted component) situated between the two primary clusters contains one Foremost Formation site (‘PK’) and four of the stratigraphically highest pre-LCZ Dinosaur Park Formation sites (‘BB102’, ‘BB119’, ‘BB108’, ‘BB115’). These stratigraphically high pre-LCZ Dinosaur Park Formation sites (along with ‘BB94’, ‘BB75’, ‘BB54’, and ‘BB120’, situated in the yellow highlighted component of Fig. 2) are positioned near the locally-variable conformable boundary between the informal lower ‘sandy’ and upper ‘muddy’ units within the pre-LCZ Dinosaur Park Formation, a transition thought to indicate the acceleration of the transgressive sequence leading into the LCZ and Bearpaw Formation [12, 50, 52]. The two primary site clusters correspond broadly to the clustering of taxa in the R-mode analysis, with the larger site cluster associated (yellow in Fig. 2) most strongly with lissamphibians (e.g. Caudata + Allocaudata), dinosaurs (e.g. Hadrosauridae, Ceratopsidae, Troodon, Dromaeosauridae, cf. Aves), and actinopterygians (e.g. ‘Holostean A’, Coriops, Teleostei, Esocidae), and the smaller primary site cluster (blue in Fig. 2) most strongly associated with batoids (e.g. Myledaphus + Pseudomyledaphus, Ischyrhiza, Protoplatyrhina, Rhinobatos), sharks (e.g. Hybodus, Archaeolamna, Odontaspidae), and actinopterygians (e.g. Belonostomus, Enchodus, Phyllodontidae). The sub-clusters within the larger (yellow in Fig. 2) of the two primary clusters are broadly similar in taxonomic composition, with the only major difference being the lack of several taxa (e.g. Paronychodon, Richardoestesia, cf. Aves) in Dinosaur Provincial Park sites. The grade of sites (green in Fig. 2) not included in the two primary clusters is associated with aquatic taxa present to varying degrees in both other clusters (e.g. Myledaphus + Pseudomyledaphus, Lepisosteus, Eusuchia, Baenidae, Champsosaurus).

Fig. 2
figure2

R-mode vs. Q-mode cluster analysis of Belly River vertebrate microfossil sites. Dinosaur Provincial Park sites indicated with blue text and triangles. Milk River/Manyberries sites indicated with red text and circles. Coloured regions note environments associated with indicated sites and taxa: yellow terrestrial, green transitional, blue marine

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Redundancy analysis

Redundancy analysis (RDA) was carried out on the percent difference dissimilarity matrix computed from the Belly River Group microsite relative abundance dataset, with stratigraphic interval (a proxy for temporal change), depositional setting (site-specific sedimentological characteristics), palaeogeographic sampling location (DPP or MRM), and palaeoenvironment (as reconstructed for the broader area or interval within the geological formation) as explanatory factors (Fig. 3). Broad overlap exists in sites preserved as crevasse splays or in-channel deposits, with these two representing the depositional setting of the vast majority of sites (Fig. 3a, red and green polygons), though sites preserved as shoreface deposits did cluster separately from other sites (Fig. 3a, blue polygon). Palaeogeographic sampling location was effective at separating site clusters in certain situations, such as for sites in the time-equivalent portion of the upper Oldman and pre-LCZ Dinosaur Park formations (Fig. 3b, dark red and blue polygons). When expanded to all sites, sampling location did not produce distinct clusters, and broad overlap was found relating to the position of lower Belly River Group sites from Milk River and upper Belly River Group sites from DPP (Fig. 3b, light red and blue polygons). When the stratigraphic interval of each site was analyzed as a clustering variable, considerable overlap was found and no directional organization could be found that would be consistent with a linear relationship through time (Fig. 3c). Sites from the lower and middle (Comrey sandstone) Oldman Formation (Fig. 3c, purple and yellow polygons) plotted adjacent to one another, with a broad overlapping distribution of sites from the upper Oldman and pre-LCZ Dinosaur Park formations (Fig. 3c, green polygon). Most Foremost Formation sites (Fig. 3c, blue polygon) plotted between pre-LCZ Dinosaur Park Formation sites from high in stratigraphic section (Fig. 3c, sites of green polygon with more negative positions on first RDA axis), near the boundary with the LCZ, and sites from within the Lethbridge Coal Zone itself (Fig. 3c, light blue polygon). The exception to this was the ‘SPS’ site, which plotted most closely to lower Oldman Formation sites. When using palaeoenvironment as a factor, sites were assigned to one of four settings, based on their lithology and predominant fauna: (1) marine, (2) transitional, (3) terrestrial (paralic; lower coastal plain), and (4) terrestrial (alluvial; upper coastal plain). The two terrestrial groupings correspond to the palaeoenvironmental conditions from which the vast majority of dinosaur fossils are known, and represent the two primary environmental regimes discussed in previous studies of dinosaur environmental sensitivity and/or provinciality/endemism [1–7, 9, 19, 20, 22–25, 36, 37, 42–45, 49, 70]. Three non-overlapping grouping were obtained: one for sites with palaeoenvironments reconstructed as marine (Fig. 3d, blue polygon), one for sites reconstructed as transitional and preserving a mix of marine and terrestrial sedimentological features and taxa (Fig. 3d, purple polygon), and a final grouping for sites reconstructed as being terrestrial (Fig. 3d, green and yellow polygons). Sites with terrestrial palaeoenvironments were further subdivided based on their prior associations with more paralic, lower coastal plains (Fig. 3d, green polygon) or more alluvial, upper coastal plains (Fig. 3d, yellow polygon). These further subdivisions followed a clustering pattern consistent with other sites, with more inland terrestrial sites plotting further from marine and transitional sites than more coastal plain terrestrial sites. Despite this trend, considerable overlap exists between more coastally influenced terrestrial sites and more alluvial terrestrial sites, indicating that the two cannot be considered truly distinct for the purpose of site clustering.

Fig. 3
figure3

Redundancy analysis of Belly River Group vertebrate microfossil site data and associated environmental factors. a Depositional setting; b palaeogeographic sampling location; c stratigraphic interval; d palaeoenvironmental setting. Subdivisions within each environmental factor noted in associated legends

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Pair-wise site assemblage similarity

Pair-wise Bray-Curtis (percentage-difference) similarity was computed for each consecutive pair of stratigraphically-ordered neighbouring sites from each sampling region (DPP and MRM), along with a visual representation of relative taxonomic group abundance at each site (Fig. 4). The Milk River/Manyberries sites (Fig. 4, red box at right) range stratigraphically from the Foremost Formation to the upper unit of the Oldman Formation (time-equivalent to the pre-LCZ Dinosaur Park Formation in DPP), and the Dinosaur Provincial Park sites (Fig. 4, blue box at left) stratigraphically range from the middle Oldman Formation (‘Comrey sandstone’) to the Lethbridge Coal Zone. Pair-wise similarity curves are relatively stable for much of the sampled intervals in both DPP (Fig. 4, blue curve) and MRM (Fig. 4, red curve), ranging from approximately 50–80% similarity. Two exceptions to this stability exist, one during the regressive phase recorded near the end of the Foremost Formation in MRM sites (Fig. 4, near base of red curve) and the other during the transgressive phase in the Lethbridge Coal Zone near the boundary between the Belly River Group and the overlying Bearpaw Formation in DPP sites (Fig. 4, near top of blue curve). In both of these cases, site similarity dropped to approximately 10–20%. Trends recorded across relative abundances of taxa in individual sites (Fig. 4, DPP sites in blue box at left and MRM sites in red box at right) and in formational average taxon abundance (Fig. 4, top right) both show that the site similarity drop in the Foremost Formation was associated with large reductions in relative abundances of chondrichthyans and large increases in relative abundances of lissamphibians, with the inverse seen in the similarity drop in the Lethbridge Coal Zone.

Fig. 4
figure4

a Pair-wise Bray-Curtis similarity of Dinosaur Provincial Park (blue square) and Milk River/Manyberries (red square) vertebrate microfossil relative abundance assemblages through Belly River Group lithostratigraphic record. b Formational average proportions of each taxonomic group in sampled regions Taxonomic groups, site identifications, and other relevant information noted in legend

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Sub-sample analyses of time-equivalent sites

Using three subsets of the broader relative abundance dataset, with re-sampled values of dinosaurs from Brinkman et al. [8] in place of Brinkman [12] for the first two, R- vs. Q-mode cluster analyses and pair-wise assemblage similarity analyses were performed for dinosaur-only (Fig. 5), theropod-only (Fig. 6), and non-dinosaur (Fig. 7) components of the assemblage. These subset comparisons were made only for sites in the overlapping stratigraphic intervals of each sampling area, namely the middle (‘Comrey sandstone’) Oldman Formation, and the upper Oldman Formation and pre-LCZ Dinosaur Park Formation.

Fig. 5
figure5

a R-mode vs. Q-mode cluster analysis of dinosaur component of Belly River Group microsites from the time-equivalent interval of the Oldman and Dinosaur Park formations of Dinosaur Provincial Park and Milk River/Manyberries. Dinosaur Provincial Park sites indicated with blue text and triangles. Milk River/Manyberries sites indicated with red text and circles. b Pair-wise Bray-Curtis similarity of Dinosaur Provincial Park (blue square) and Milk River/Manyberries (red square) microsite dinosaur relative abundance assemblages through lithostratigraphic record of time-equivalent Oldman and Dinosaur Park formations. c Formational average proportions of each dinosaur group in sampled regions. Taxonomic groups, site identifications, and other relevant information noted in legend

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Fig. 6
figure6

a R-mode vs. Q-mode cluster analysis of theropod component of Belly River Group microsites from the time-equivalent interval of the Oldman and Dinosaur Park formations of Dinosaur Provincial Park and Milk River/Manyberries. Dinosaur Provincial Park sites indicated with blue text and triangles. Milk River/Manyberries sites indicated with red text and circles. b Pair-wise Bray-Curtis similarity of Dinosaur Provincial Park (blue square) and Milk River/Manyberries (red square) microsite theropod relative abundance assemblages through lithostratigraphic record of time-equivalent Oldman and Dinosaur Park formations. c Formational average proportions of each theropod group in sampled regions. Taxonomic groups, site identifications, and other relevant information noted in legend

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Fig. 7
figure7

a R-mode vs. Q-mode cluster analysis of non-dinosaur component of Belly River Group microsites from the time-equivalent interval of the Oldman and Dinosaur Park formations of Dinosaur Provincial Park and Milk River/Manyberries. Dinosaur Provincial Park sites indicated with blue text and triangles. Milk River/Manyberries sites indicated with red text and circles. b Pair-wise Bray-Curtis similarity of Dinosaur Provincial Park (blue square) and Milk River/Manyberries (red square) microsite non-dinosaurian vertebrate relative abundance assemblages through lithostratigraphic record of time-equivalent Oldman and Dinosaur Park formations. c Formational average proportions of each non-dinosaurian vertebrate group in sampled regions. Taxonomic groups, site identifications, and other relevant information noted in legend

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In the dinosaur sub-sample (Fig. 5), hadrosaurs constituted the vast majority of assemblage relative abundance in almost all sites from both Dinosaur Provincial Park and Milk River. The only exception to this was the DPP ‘BB54’ site, which clustered away from all other sites (Fig. 5A). With the exception of hadrosaurs, no single dinosaur taxon was found to be driving large-scale site clustering, though increased Troodon relative abundance seems to drive the finer-scale clustering of two MRM sites (‘RDS’ and ‘CBC’) and a DPP site (‘BB71’), and relative abundance of ankylosaurs drives the clustering of two DPP sites (‘BB86’ and ‘BB100’) and one MRM site (‘PLS’). Unlike in the cluster analyses of the broader vertebrate assemblages, there is less distinct clustering of sites by sampling region (Fig. 5a, MRM sites indicated by red circles and DPP sites indicated by blue triangles). Pair-wise site similarity for dinosaur assemblages in the sub-sampled interval is relatively stable for both DPP and MRM, ranging approximately 40–80% similarity (Fig. 5b, DPP sites in blue box and represented by blue similarity curve and MRM sites in red box and represented by red similarity curve). The only deviation from this trend is a drop to approximately 20% similarity for sites neighbouring ‘BB54’, which as noted above represents an apparent outlier due to a lower relative abundance of hadrosaurs, and much higher relative abundances of ceratopsians and Richardoestesia (Fig. 5b). Formational average abundances of dinosaurs (Fig. 5c) do not show any major shifts in assemblage between the middle Oldman Formation sites and the sites of the time-equivalent upper Oldman and pre-LCZ Dinosaur Park formations, nor is there considerable difference in assemblages between the DPP and Milk River localities. The only exceptions to this are a moderate increase in ceratopsians in DPP between the middle Oldman Formation (~1% relative abundance) and time-equivalent pre-LCZ Dinosaur Park Formation (~7% relative abundance), a shift also found in the equivalent intervals of the Milk River sites (~4 to ~7% relative abundance, respectively). In the middle Oldman Formation and time-equivalent upper Oldman Formation of Milk River, there were also changes in small theropod relative abundances, with saurornitholestines increasing slightly (~1 to ~5%), and Troodon going from absent to present (with relative abundance in the upper Oldman Formation of ~1%).

The theropod-only sub-sample analyses (Fig. 6) produced similar results to the sub-sample of dinosaurs. Most sites did not show any strong signal from a particular taxon driving clustering patterns (Fig. 6a), though a few clustered together due to their greater association with tyrannosaurids and Richardoestesia (‘BB120’, ‘BB115’, ‘BB75’, BB119) or with cf. Aves (‘BB61’, ‘CN-2’, ‘ORS’, ‘HS’). As with dinosaurs, the site similarity curves of the theropod sub-samples from DPP and MRM are very similar (Fig. 6b), both staying within a range of ~30 to ~80% similarity. The lower bound of that similarity range related to sites neighbouring those with very little theropod material (e.g. ‘BB120’, ‘BB75’, ‘BB115’, and ‘BB119’). The formational average theropod relative abundances (Fig. 6c) in DPP show no appreciable differences, with slight increases in proportions of tyrannosaurids and Richardoestesia, and slight decrease in Troodon. In MRM, there are more considerable differences in formational average relative theropod abundances, with saurornitholestine proportions greatly increasing, Troodon appearing in the upper Oldman Formation while not being found in the middle Oldman Formation, and all other taxa proportionally decreasing slightly.

The non-dinosaur sub-sample analysis (Fig. 7) produced similar results to the non-marine components of the R- vs. Q-mode analysis of all microsite data (Fig. 2, yellow square). Clustering of sites based on their provenience was apparent, though sites did not cluster exclusively based on being from DPP or MRM (Fig. 7a). Sites from MRM clustered more closely to the majority of DPP sites than a number of DPP sites (e.g. ‘BB106’, ‘BB117’, ‘BB121’, ‘BB94’, ‘BB75’, ‘BB119’, ‘BB102’), with those latter sites forming a cluster more similar to each other than to any other site. This cluster was associated the proportion of particular actinopterygians (e.g. Lepisosteus, Paratarpon, Acipenseriformes, Belonostomus), and turtles (e.g. Basilemys, Baenidae, Trionychidae). Two sites, both stratigraphically high in the DPF and close to the LCZ, were associated with batoids (e.g. Myledaphus + Pseudomyledaphus), Basilemys, and in one case sharks (Hybodus, in ‘BB115’). As in other analyses, ‘BB54’ grouped as something of an outlier, and was here associated strongly with ‘Holostean A’ and ‘Holostean B’. Other DPP sites were broadly associated with many taxa, though in particular with actinopterygians (e.g. Coriops, ‘Holostean A’), baenid turtles, and lissamphibians (e.g. Caudata + Allocaudata). Sites sampled from MRM as a whole were distinguished from DPP mainly due to an even stronger association with lissamphibians (e.g. Caudata + Allocaudata) and certain actinopterygians (e.g. esocids, teleosts), though particular MRM sites were also distinguished based on their association with taxa that were either absent or in low abundance at other sites, particularly those from DPP. For example, Adocus and Chiloscyllium are strongly associated with the ‘CS’ site, though absent or in very low abundance in most sites. One MRM site (‘BMC’) clustered as an outlier, and was distinguished through a suite of taxa (e.g. chelydrid turtles, mammals, anurans, squamates). Both of these outlier sites (‘BB54’ and ‘BMC’) have been noted in previous research to be sedimentologically distinct from other sites, possibly representing an exception to the general trend of depositional setting having a relatively small effect on microsite assemblage structure [14, 52]. Site similarity curves for DPP and MRM (Fig. 7b) were very similar, and very stable, both fluctuating around 60% similarity for much of the sampled interval. The only prominent exception to this came at the top of the time-equivalent interval in DPP, where similarity began to steadily drop, reaching approximately 40% similarity by the top of the sampled interval. Overall proportions of major taxonomic groups in DPP and MRM during this interval (Fig. 7c) were similar, with the notable exception being the higher proportion of batoids in DPP (<20%) when compared to MRM (<5%).

Discussion

Drivers of faunal assemblage clustering in the Belly River Group

Our results broadly support the conclusions of previous studies such as Brinkman et al. [14], expand on their work to include sites from both MRM and DPP in a series of analyses, and more thoroughly test the role that various abiotic factors play in structuring the preserved faunal assemblages. The results of the R- vs. Q-mode cluster analyses (Fig. 2), RDA analyses (Fig. 3), and pair-wise site similarity comparisons (Fig. 4) for the full sample of microsite faunal assemblages of MRM and DPP indicate that palaeoenvironmental changes, particularly marine-terrestrial transitions, are responsible for the most significant changes in faunal assemblage structure, and that other factors like site depositional setting, relative stratigraphic position within the Belly River Group, and palaeogeographic sampling location played a lesser to negligible role. While depositional setting (Fig. 3a) appears superficially to explain site clustering, it should be noted that the only depositional setting that did not display considerable overlap with others were shoreface deposits, and each site characterized as a shoreface deposit was also characterized as preserving a primarily marine palaeoenvironment. Of the three depositional settings analyzed, there was broad overlap in sites characterized as crevasse splays or in-channel deposits (Fig. 3a), which is consistent with palaeoenvironment (Fig. 3d) driving site clustering, as there is also considerable overlap in terrestrial sites, all of which in this sample are preserved as either in-channel or crevasse splay deposits. The differing depositional characteristics of these terrestrial sites are therefore not having a strong effect on the preserved faunal assemblage. Palaeogeographic sampling location appears to also have some effect on faunal assemblage structure, at least within sites from the time-equivalent interval of the Oldman and pre-LCZ Dinosaur Park formations of Dinosaur Provincial Park and Milk River (Fig. 3b). In that context, the DPP and MRM sites formed distinct clusters, though this separation did not hold when expanded to the complete sample of Belly River Group microsites. The separate timeequivalent DPP and MRM clusters provide further support to the hypothesis that at least some of the differences in microsite faunal assemblage structure is the result of biogeographic differences related to environmental variation across the palaeolandscape [12, 14]. However, the distinct clustering of these sites may also be an artefact related to the absence of several taxa (e.g. Paronychodon, cf. Aves, Richardoestesia) in the DPP microsite data that are moderately abundant in MRM sites, despite these taxa being reported in the Dinosaur Park Formation [71]. This effect is similarly seen in the PHRN site (Fig. 2) being associated with ‘Theropoda indet’ material (alongside the numerous marine chondrichthyan taxa that primarily characterize the site) due to this taxonomic category only existing for this site (based on the source data).

Overall, the results of these analyses for the entire Belly River Group microsite database build on prior research conducted on this subject [8, 12–14, 16, 53, 58, 61], and serve to more thoroughly and quantitatively establish the patterns that have been observed in this system. It is not particularly surprising that changes in sea level in the Belly River Group acted as a strong driver of environmental and faunal assemblage change, as the most significant change in faunal assemblage during these intervals is the inverse proportional change in chondrichthyans and lissamphibians, which is almost certainly related to the degree of marine preference (or lack-thereof in the latter case) in these taxa [16, 72, 73]. However, it is important to quantify and understand the exact nature of these trends, as these controvertial data form the baseline for future comparisons and facilitate the testing of more controversial questions, such as the environmental sensitivity of dinosaurs and the effects that more subtle environmental variation have on local palaeocommunity structure.

Altitudinal and latitudinal sensitivity of dinosaur assemblages

The sensitivity of dinosaur populations to changes in altitudinal (distance from palaeoshoreline) and latitudinal environmental gradients has been the subject of considerable debate for over 30 years [1–9, 11, 15, 23, 26, 27, 42–46, 55, 62, 70], and although it has been questioned [17, 18, 22, 28, 74], it remains one of the primary explanations for patterns observed in the evolution and distribution of dinosaurs throughout the Late Cretaceous of western North America. A focused sub-sampling of the time-equivalent interval of the Oldman and Dinosaur Park formations within the larger Belly River Group microsite abundance dataset facilitates a controlled and direct test of dinosaur assemblage changes across differing palaeoenvironments (Figs. 5, 6), while also allowing comparisons to the non-dinosaur component of the broader vertebrate assemblage (Fig. 7) albeit at a regional, rather than continental, scale.

Within dinosaurs (Fig. 5) there is broad assemblage stability, despite the sampled regions representing differing terrestrial environments (lower coastal plain vs. upper coastal plain/inland alluvial fan). In both DPP and MRM, hadrosaurs dominate the preserved dinosaur assemblages, often representing over 80% of relative abundance. A moderate increase in ceratopsians is noted through time in DPP, though across time-equivalent intervals the proportional difference between ceratopsians in DPP and MRM is negligible. This stability between sampling areas is also generally seen in the theropods (Fig. 6), with Troodon representing the main exception, as it is not present in the earliest MRM site, appearing there later than in DPP. This was also noted by Brinkman et al. [14], and was attributed to southward migration/range-expansion. The later appearance of Troodon in MRM, along with an increase in saurornitholestine material from the middle to upper Oldman Formation in MRM, may be a genuine change in theropod assemblage that is not seen in DPP, or it may be a result of sampling issues, as the middle Oldman Formation of the MRM area is only represented by a single site (‘ORS’). The non-dinosaur component of the vertebrate faunal assemblage was relatively stable across much of the sampled interval, although there were considerable differences in the relative abundances of taxa between DPP and MRM (Fig. 7), with batoids forming a much larger component of the overall fauna in DPP. Unlike the pattern shown in the broader vertebrate assemblages (Fig. 7), there does not appear to be strong clustering according to sampling region in dinosaurs (Figs. 5a, 6a), suggesting that the palaeogeographic signal in the broader analysis is due to abundance differences (e.g. batoids) or rare/endemic taxa of non-dinosaurian affiliation (e.g. lissamphibians, turtles, etc.). The similarity of faunal assemblages, and particularly dinosaur assemblages, within the two terrestrial palaeoenvironmental settings (coastal plain vs. alluvial/inland) indicates that these subtle variations in terrestrial palaeoenvironment may have less effect on faunal assemblage structure than previously suggested [1, 12, 14], a position supported by recent research on ceratopsids in the Oldman Formation of the Milk River/Manyberries region [22]. It is also possible that the palaeoenvironmental interpretation of these formations and sampling areas is more dynamic than originally described [50], though, pending future geological investigations, there is currently no reason to think this is the case. The relative similarity of the dinosaur faunal assemblages of DPP and MRM, and how those contrast to the differences seen in the rest of the vertebrate faunal assemblage between these areas and throughout the extent of the Belly River Group, does not support the idea that dinosaurs, including large bodied taxa like hadrosaurs and ceratopsians, are sensitive to relatively small environmental changes across the regional palaeoenvironmental landscape [1–8, 11, 19–24, 27, 39, 43, 70]. How this plays out over continental scales and between basins is currently unclear, but at least in terms of community composition measured at the family level, large bodied herbivore communities seem to exhibit little variation over the altitudinal transects considered here.

Conclusions

The results of this study demonstrate that palaeoenvironmental setting is the primary driver of differences in vertebrate faunal assemblages throughout the Belly River Group, with palaeogeography/palaeolandscape acting as another factor in structuring these assemblages. Depositional setting and stratigraphic interval do not have particularly strong effects on the preserved faunal assemblage, confirming the results of other smaller-scale studies [14, 59].

The subsample analyses of dinosaur and theropod assemblages, and their comparisons to the broader vertebrate assemblages, suggest one of two possible conclusions: either (a) dinosaur community composition is not sensitive to subtle changes in altitudinal and latitudinal palaeoenvironmental gradients, and/or (b) the differences in environment between the pre-LCZ Dinosaur Park Formation of DPP and the upper Oldman Formation of MRM have been overstated. The higher proportion of batoids in DPP than MRM across this same interval suggests that the more coastally-influenced terrestrial environment of DPP is genuine, refuting the long-held idea that dinosaur communities were particularly sensitive to small-scale environmental gradients, such as paralic (coastal) to alluvial (inland) regimes within a single depositional basin. Further research is required to fully answer this question, but it is possible that consistently high rates of evolution and niche partitioning among species within each of the sampled dinosaur families played a greater role in the observed high diversity and frequent turnovers in dinosaur taxa throughout the Late Cretaceous of North America.

Abbreviations

BRG:

Belly River Group

DPF:

Dinosaur Park Formation

DPP:

Dinosaur Provincial Park

LCZ:

Lethbridge Coal Zone

MRM:

Milk River/Manyberries

OM:

Oldman Formation

RDA:

redundancy analysis

UPGMA:

unweighted pair group method with arithmetic mean

References

  1. 1.

    Gates TA, Sampson SD, Zanno LE, Roberts EM, Eaton JG, Nydam RL, Hutchison JH, Smith JA, Loewen MA, Getty MA. Biogeography of terrestrial and freshwater vertebrates from the late Cretaceous (Campanian) Western Interior of North America. Palaeogeogr Palaeoclimatol Palaeoecol. 2010;291(3–4):371–87.

    Article  Google Scholar 

  2. 2.

    Lehman TM. Late Cretaceous dinosaur provinciality. In: Tanke D, Carpenter K, editors. Mesozoic vertebrate life. Ottawa: NRC Research Press; 2001. p. 310–28.

    Google Scholar 

  3. 3.

    Sampson SD, Loewen MA, Farke AA, Roberts EM, Forster CA, Smith JA, Titus AL. New horned dinosaurs from Utah provide evidence for intracontinental dinosaur endemism. PLoS ONE. 2010;5(9):e12292.

    Article  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Gates TA, Prieto-Márquez A, Zanno LE. Mountain building triggered late Cretaceous North American megaherbivore dinosaur radiation. PLoS ONE. 2012;7(8):e42135.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  5. 5.

    Horner J, Varricchio DJ, Goodwin MB. Marine transgressions and the evolution of Cretaceous dinosaurs. Nature. 1992;358:59–61.

    Article  Google Scholar 

  6. 6.

    Lehman TM. Late Maastrichtian paleoenvironments and dinosaur biogeography in the western interior of North America. Palaeogeogr Palaeoclimatol Palaeoecol. 1987;60:189–217.

    Article  Google Scholar 

  7. 7.

    Lehman TM. Late Campanian dinosaur biogeography in the western interior of North America. Dinofest Int. 1997;1997:223.

    Google Scholar 

  8. 8.

    Brinkman DB, Ryan MJ, Eberth DA. The paleogeographic and stratigraphic distribution of ceratopsids (Ornithischia) in the Upper Judith River Group of Western Canada. Palaios. 1998;13(2):160–9.

    Article  Google Scholar 

  9. 9.

    Loewen MA, Irmis RB, Sertich JJ, Currie PJ, Sampson SD. Tyrant dinosaur evolution tracks the rise and fall of Cretaceous oceans. PLoS ONE. 2013;8(11):e79420.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  10. 10.

    Mallon JC, Evans DC, Ryan MJ, Anderson JS. Feeding height stratification among the herbivorous dinosaurs from the Dinosaur Park Formation (upper Campanian) of Alberta, Canada. BMC Ecology. 2013;13(1):1–15.

    Article  Google Scholar 

  11. 11.

    Carrano MT. Dinosaurian faunas of the later Mesozoic. In: Brett-Surman MK, Holtz Jr TR, Farlow JO, editors. The complete dinosaur. 2nd ed. Bloomington: Indiana University Press; 2012. p. 1003–26.

    Google Scholar 

  12. 12.

    Brinkman DB. Paleoecology of the Judith River Formation (Campanian) of Dinosaur Provincial Park, Alberta, Canada: Evidence from vertebrate microfossil localities. Palaeogeogr Palaeoclimatol Palaeoecol. 1990;78:37–54.

    Article  Google Scholar 

  13. 13.

    Beavan NR, Russell AP. An elasmobranch assemblage from the terrestrial-marine transitional Lethbridge Coal Zone (Dinosaur Park Formation: Upper Campanian), Alberta. Canada. J Paleontol. 1999;73(3):494–503.

    Article  Google Scholar 

  14. 14.

    Brinkman DB, Russell AP, Eberth DA, Peng JH. Vertebrate palaeocommunities of the lower Judith River Group (Campanian) of southeastern Alberta, Canada, as interpreted from vertebrate microfossil assemblages. Palaeogeogr Palaeoclimatol Palaeoecol. 2004;213(3–4):295–313.

    Article  Google Scholar 

  15. 15.

    Fanti F, Miyashita T. A high latitude vertebrate fossil assemblage from the Late Cretaceous of west-central Alberta, Canada: evidence for dinosaur nesting and vertebrate latitudinal gradient. Palaeogeogr Palaeoclimatol Palaeoecol. 2009;275(1–4):37–53.

    Article  Google Scholar 

  16. 16.

    Cullen TM, Fanti F, Capobianco C, Ryan MJ, Evans DC. A vertebrate microsite from a marine-terrestrial transition in the Foremost Formation (Campanian) of Alberta, Canada, and the use of faunal assemblage data as a paleoenvironmental indicator. Palaeogeogr Palaeoclimatol Palaeoecol. 2016;444:101–14.

    Article  Google Scholar 

  17. 17.

    Butler RJ, Benson RB, Carrano MT, Mannion PD, Upchurch P. Sea level, dinosaur diversity and sampling biases: investigating the ‘common cause’ hypothesis in the terrestrial realm. Proc R Soc Lond B Biol Sci. 2011;278(1709):1165–70.

    Article  Google Scholar 

  18. 18.

    Dunhill AM, Bestwick J, Narey H, Sciberras J. Dinosaur biogeographical structure and Mesozoic continental fragmentation: a network-based approach. J Biogeogr. 2016;43:1691–704. doi:10.1111/jbi.12766.

    Article  Google Scholar 

  19. 19.

    Horner J. The Mesozoic terrestrial ecosystem of Montana. In: Montana Geological Society Field Conference; 1989.

  20. 20.

    Horner J. Three ecologically distinct vertebrate faunal communities from the Late Cretaceous two medicine formation of Montana, with discussion of evolutionary pressures induced by interior seaway fluctuations. In: Montana Geological Society Field Conference; 1984.

  21. 21.

    Bell PR, Currie PJ. A high-latitude dromaeosaurid, Boreonykus certekorum, gen. et sp. nov. (Theropoda), from the upper Campanian Wapiti Formation, west-central Alberta. J Vertebr Paleontol. 2016;36(1):e1034359.

    Article  Google Scholar 

  22. 22.

    Chiba K, Ryan MJ, Braman DR, Eberth DA, Scott EE, Brown CM, Kobayashi Y, Evans DC. Taphonomy of a monodominant Centrosaurus apertus (Dinosauria: Ceratopsia) bonebed from the upper Oldman Formation of southeastern Alberta. Palaios. 2015;30(9):655–67.

    Article  Google Scholar 

  23. 23.

    Evans D, Currie P, Eberth D, Ryan M. High-resolution lambeosaurine dinosaur biostratigirphy, Dinosaur Park Formation, Alberta: sexual dimorphism reconsidered. J Vertebr Paleontol. 2006;26(3):59A.

    Google Scholar 

  24. 24.

    Evans DC, McGarrity CT, Ryan MJ. A skull of Prosaurolophus maximus from Southeastern Alberta and the spatiotemporal distribution of faunal zones in the Dinosaur Park Formation. In: Eberth D, Evans DC, editors. Hadrosaurs. Bloomington: Indiana University Press; 2014. p. 200–7.

    Google Scholar 

  25. 25.

    Lucas SG. Dinosaur communities of the San Juan Basin: A case for lateral variations in the composition of Late Cretaceous dinosaur communities. In: Lucas SG, Rigby K, Kues BS, editors. Advances in San Juan Basin Paleontology. Albuquerque: University of New Mexico Press; 1981. p. 337–93.

    Google Scholar 

  26. 26.

    Prieto-Marquez A, Wagner JR, Bell PR, Chiappe LM. The late-surviving ‘duck-billed’dinosaur Augustynolophus from the upper Maastrichtian of western North America and crest evolution in Saurolophini. Geol Mag. 2015;152(02):225–41.

    Article  Google Scholar 

  27. 27.

    Rivera-Sylva HE, Hedrick BP, Dodson P. A Centrosaurine (Dinosauria: Ceratopsia) from the Aguja Formation (Late Campanian) of Northern Coahuila, Mexico. PLoS ONE. 2016;11(4):e0150529.

    Article  PubMed  PubMed Central  Google Scholar 

  28. 28.

    Sullivan RM, Lucas SG. The Kirtlandian land-vertebrate “age”-faunal composition, temporal position and biostratigraphic correlation in the nonmarine Upper Cretaceous of western North America. NM Mus Na Hist Sci Bull. 2006;35:7–30.

    Google Scholar 

  29. 29.

    Béland P, Russell DA. Paleoecology of Dinosaur Provincial Park (Cretaceous), Alberta, interpreted from the distribution of articulated vertebrate remains. Can J Earth Sci. 1012;1978:15.

    Google Scholar 

  30. 30.

    Coe MJ, Dilcher DL, Farlow JO, Jarzen DM, Russell DA. Dinosaurs and land plants. In: Friis EM, Chaloner WG, Crane PR, editors. The origins of Angiosperms and their biological consequences. Cambridge: Cambridge University Press; 1987.

    Google Scholar 

  31. 31.

    Currie PJ, Dodson P. Mass death of a heard of ceratopsian dinosaurs. In: Reif WE, Westphal F, editors. Third symposium of Mesozoic terrestrial ecosystems. Tübingen: Attempto Verlag; 1984.

    Google Scholar 

  32. 32.

    du Toit JT, Cumming DHM. Functional significance of ungulate diversity in African savannas and the ecological implications of the spread of pastoralism. Biodivers Conserv. 1999;8:1643.

    Article  Google Scholar 

  33. 33.

    Farlow JO. A consideration of the trophic dynamics of a Late Cretaceous large-dinosaur community (Oldman Formation). Ecology. 1976;57:841.

    Article  Google Scholar 

  34. 34.

    Farlow JO, Dodson P, Chinsamy A. Dinosaur biology. Annu Rev Ecol Syst. 1995;26:445.

    Article  Google Scholar 

  35. 35.

    Horner JR, Makela R. Nest of juveniles provides evidence of family structure among dinosaurs. Nature. 1979;282:296–8. doi:10.1038/282296a0.

    Article  Google Scholar 

  36. 36.

    Ryan MJ, Russell AP, Eberth DA, Currie PJ. The taphonomy of a Centrosaurus (Ornithischia: Ceratopsidae) bone bed from the Dinosaur Park Formation (Upper Campanian), Alberta, Canada, with comments on cranial ontogeny. PALAIOS. 2001;16:482.

    Article  Google Scholar 

  37. 37.

    Varricchio DJ, Horner JR. Hadrosaurid and lambeosaurid bone beds from the Upper Cretaceous Two Medicine Formation of Montana: taphonomic and biologic implications. Can J Earth Sci. 1993;30:997.

    Article  Google Scholar 

  38. 38.

    Bakker RT. Dinosaur feeding behaviour and the origin of flowering plants. Nature. 1978;274:661–3. doi:10.1038/274661a0.

    Article  Google Scholar 

  39. 39.

    Campione NE, Evans DC. Cranial growth and variation in Edmontosaurus (Dinosauria: Hadrosauridae): implications for latest Cretaceous megaherbivore diversity in North America. PLoS ONE. 2011;6:e25186.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  40. 40.

    Carrano MT, Janis CM, Sepkoski JJ. Hadrosaurs as ungulate parallels: lost lifestyles and deficient data. Acta Palaeontol Pol. 1999;44:237.

    Google Scholar 

  41. 41.

    Dodson P. A faunal review of the Judith River (Oldman) Formation, Dinosaur Provincial Park, Alberta. Mosasaur. 1983;1:89.

    Google Scholar 

  42. 42.

    Fricke HC, Pearson DA. Stable isotope evidence for changes in dietary niche partitioning among hadrosaurian and ceratopsian dinosaurs of the Hell Creek Formation, North Dakota. Paleobiology. 2008;34(4):534–52.

    Article  Google Scholar 

  43. 43.

    Mallon JC, Evans DC, Ryan MJ, Anderson JS. Megaherbivorous dinosaur turnover in the Dinosaur Park Formation (upper Campanian) of Alberta, Canada. Palaeogeogr Palaeoclimatol Palaeoecol. 2012;350–352:124–38.

    Article  Google Scholar 

  44. 44.

    Ryan MJ, Evans DC. Ornithischian Dinosaurs. In: Currie PJ, Koppelhus EB, editors. Dinosaur Provincial Park: a spectacular ancient ecosystem revealed. Bloomington: Indiana Univeristy Press; 2005. p. 312–48.

    Google Scholar 

  45. 45.

    Sampson SD, Loewen MA. Unraveling a radiation: a review of the diversity, stratigraphic distribution, biogeography, and evolution of horned dinosaurs (Ornithischia: Ceratopsidae). In: Ryan MJ, Chinnery-Allgeier BJ, Eberth DA, editors. New perspectives on horned dinosaurs: the royal tyrrell museum Ceratopsian symposium. Bloomington: Indiana University Press; 2010.

    Google Scholar 

  46. 46.

    Longrich NR. Judiceratops tigris, a New Horned Dinosaur from the Middle Campanian Judith River Formation of Montana. Bull Peabody Mus Nat Hist. 2013;54(1):51–65.

    Article  Google Scholar 

  47. 47.

    Evans DC, Reisz RR. Anatomy and relationships of Lambeosaurus magnicristatus, a crested hadrosaurid dinosaur (Ornithischia) from the Dinosaur Park Formation, Alberta. J Vertebr Paleontol. 2007;27(2):373–93.

    Article  Google Scholar 

  48. 48.

    Currie PJ, Koppelhus EB, editors. Dinosaur Provincial Park: a spectacular ancient ecosystem revealed. Bloomington: Indiana University Press; 2005.

    Google Scholar 

  49. 49.

    Eberth DA. The Geology. In: Currie PJ, Koppelhus EB, editors. Dinosaur Provincial Park: a spectacular ancient ecosystem revealed. Bloomington: Indiana University Press; 2005. p. 54–82.

    Google Scholar 

  50. 50.

    Eberth DA, Hamblin AP. Tectonic, stratigraphic, and sedimentologic significance of a regional discontinuity in the upper Judith River Group (Belly River wedge) of southern Alberta, Saskatchewan, and northern Montana. Can J Earth Sci. 1993;30:174–200.

    Article  Google Scholar 

  51. 51.

    Ogunyomi O, Hills LV. Depositional environments, Foremost Formation (Late Cretaceous), Milk River area, southern Alberta. Bull Can Pet Geol. 1977;25(5):929–68.

    Google Scholar 

  52. 52.

    Eberth DA. Stratigraphy and sedimentology of vertebrate microfossil sites in the uppermost Judith River Formation (Campanian), Dinosaur Provincial Park, Alberta, Canada. Palaeogeogr Palaeocl. 1990;78:1–36.

    Article  Google Scholar 

  53. 53.

    Brinkman DB, Braman DR, Neuman AG, Ralrick PE, Sato T. A vertebrate assemblage from the marine shales of the Lethbridge Coal Zone. In: Currie PJ, Koppelhus EB, editors. Dinosaur Provincial Park: a spectacular ancient ecosystem revealed. Bloomington: Indiana University Press; 2005. p. 486–500.

    Google Scholar 

  54. 54.

    Braman DR, Koppelhus EB. Campanian palynomorphs. In: Currie PJ, Koppelhus EB, editors. Dinosaur Provincial Park: a spectacular ancient ecosystem revealed. Bloomington: Indiana University Press; 2005. p. 101–30.

    Google Scholar 

  55. 55.

    Larson DW, Brinkman DB, Bell PR. Faunal assemblages from the upper Horseshoe Canyon Formation, an early Maastrichtian cool-climate assemblage from Alberta, with special reference to the Albertosaurus sarcophagus bonebed This article is one of a series of papers published in this Special Issue on the theme Albertosaurus. Can J Earth Sci. 2010;47(9):1159–81.

    Article  Google Scholar 

  56. 56.

    Sankey JT, Brinkman D, Guenther M, Currie PJ. Small theropod and bird teeth from the late Cretaceous (late Campanian) Judith River Group, Alberta. J Paleontol. 2002;76(4):751–63.

    Article  Google Scholar 

  57. 57.

    Eberth D, Shannon M, Noland B. A bonebeds database: classification, biases, and patterns of occurrence. In: Rogers R, Eberth D, Fiorillo A, editors. Bonebeds: genesis, analysis, and paleobiological significance. Chicago: University of Chicago Press; 2007. p. 103–220.

    Google Scholar 

  58. 58.

    Peng J, Russell AP, Brinkman DB. Vertebrate microsite assemblages (exclusive of mammals) from the Foremost and Oldman Formations of the Judith River Group (Campanian) of southeastern Alberta: an illustrated guide. Prov Mus Alberta Nat His Occ Papers. 2001;25:1–54.

    Google Scholar 

  59. 59.

    Rogers RR, Brady ME. Origins of microfossil bonebeds: insights from the Upper Cretaceous Judith River Formation of north-central Montana. Paleobiology. 2010;36(1):80–112.

    Article  Google Scholar 

  60. 60.

    Rogers RR, Kidwell SM. A conceptual framework for the genesis and analysis of vertebrate skeletal concentrations. In: Rogers RR, Eberth DA, Fiorillo AR, editors. Bonebeds: genesis, analysis, and paleobiological significance. Chicago: University of Chicago Press; 2007. p. 1–63.

    Google Scholar 

  61. 61.

    Frampton EK. Taphonomy and palaeoecology of mixed invertebrate-vertebrate fossil assemblage in the Foremost Formation (Cretaceous, Campanian), Milk River Valley, Alberta. Calgary: University of Calgary; 2005.

    Google Scholar 

  62. 62.

    Bell PR, Snively E. Polar dinosaurs on parade: a review of dinosaur migration. Alcheringa. 2008;32(3):271–84.

    Article  Google Scholar 

  63. 63.

    Kirkland JJ, Eaton JG, Brinkman DB. Elasmobranchs from Upper Cretaceous freshwater facies in southern Utah. In: Loewen MA, Titus AL, editors. At the top of the Grand Staircase—the Late Cretaceous of southern Utah. Bloomington: Indiana University Press; 2013. p. 153–94.

    Google Scholar 

  64. 64.

    Newbrey MG, Murray AM, Brinkman DB, Wilson MVH, Neuman AG. A new articulated freshwater fish (Clupeomorpha, Ellimmichthyiformes) from the Horseshoe Canyon Formation, Maastrichtian, of Alberta, Canada. Can J Earth Sci. 2010;47(9):1183–96.

    Article  Google Scholar 

  65. 65.

    Oksanen J, Blanchet FG, Kindt R, Legendre P, Minchin PR, O’Hara RB, Simpson GL, Solymos P, Stevens MH, Wagner H. Vegan: Community Ecology Package. In., 2.0-10 edn; 2013.

  66. 66.

    De Cáceres M, Legendre P, He F. Dissimilarity measurements and the size structure of ecological communities. Methods Ecol Evol. 2013;4:1167–77.

    Article  Google Scholar 

  67. 67.

    Wickham H. ggplot2: elegant graphics for data analysis. New York: Springer; 2009.

    Google Scholar 

  68. 68.

    Wickham H. Package ‘reshape2, flexibly reshape data: a reboot of the reshape package. In., 1.4.1 edn; 2014.

  69. 69.

    Vavrek MJ. Package ‘fossil’, palaeoecological and palaeogeographical analysis tools. In., 0.3.7 edn; 2012.

  70. 70.

    Ryan MJ, Evans DC, Shepherd KM. A new ceratopsid from the Foremost Formation (middle Campanian) of Alberta. Can J Earth Sci. 2012;49(11):1251–62.

    Article  Google Scholar 

  71. 71.

    Brown CM, Evans DC, Campione NE, O’Brien LJ, Eberth DA. Evidence for taphonomic size bias in the Dinosaur Park Formation (Campanian, Alberta), a model Mesozoic terrestrial alluvial-paralic system. Palaeogeogr Palaeoclimatol Palaeoecol. 2013;372:108–22.

    Article  Google Scholar 

  72. 72.

    Christman SP. Geographic variation for salt water tolerance in the frog Rana sphenocephalia. Copeia. 1974;3:773–8.

    Article  Google Scholar 

  73. 73.

    Smith HW. Water regulation and its evolution in the fishes. Q Rev Biol. 1932;7(1):1–26.

    CAS  Article  Google Scholar 

  74. 74.

    Vavrek MJ, Larsson HC. Low beta diversity of Maastrichtian dinosaurs of North America. Proc Natl Acad Sci USA. 2010;107(18):8265–8.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

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Authors’ contributions

TMC and DCE conceived and designed the project; TMC assembled the data and performed the analyses; TMC made the figures and tables. TMC and DCE wrote the manuscript. All authors read and approved the final manuscript.

Acknowledgements

We would like to thank previous researchers on Belly River Group microsites whose data we utilized, and in particular Don Brinkman for his peerless contributions to vertebrate microfossil palaeoecology. David Eberth and Don Brinkman are thanked for their helpful comments on an earlier draft of this manuscript. Michael J. Ryan, Derek Larson, Karma Nanglu, Kentaro Chiba, Caleb Brown, Kirstin Brink, Jordan Mallon, and Nicolas Campione are thanked for additional discussions related to this research. We also thank A. Dunhill and T. Tortosa for their helpful reviewer comments.

Competing interests

The authors declare that they have no competing interests.

Availability of data and materials

All data pertaining to this project and supporting the results are included within the article and in Additional files 1 and 2.

Consent to participate

This study did not involve research on human subjects.

Ethics

This study did not involve regulated animal species. All specimens analyzed in this project represent the fossilized remains of ancient/extinct species.

Funding

Funding provided to T.M.C through a Natural Sciences and Engineering Research Council Alexander Graham Bell Canada Graduate Scholarship (CGSD3-444747-2013), and to D.C.E through a Natural Sciences and Engineering Research Council Discovery Grant (RGPIN 355845).

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Affiliations

  1. Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, ON, Canada

    Thomas M. Cullen & David C. Evans

  2. Department of Natural History, Royal Ontario Museum, 100 Queen’s Park, Toronto, ON, M5S 2C6, Canada

    Thomas M. Cullen & David C. Evans

Authors
  1. Thomas M. Cullen
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  2. David C. Evans
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Correspondence to Thomas M. Cullen.

Additional information

The original version of this article was revised.

An erratum to this article is available at http://dx.doi.org/10.1186/s12898-016-0111-y.

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Cullen, T.M., Evans, D.C. Palaeoenvironmental drivers of vertebrate community composition in the Belly River Group (Campanian) of Alberta, Canada, with implications for dinosaur biogeography. BMC Ecol 16, 52 (2016). https://doi.org/10.1186/s12898-016-0106-8

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Keywords

  • Palaeoenvironments
  • Vertebrate microfossil sites
  • Palaeoecology
  • Altitudinal sensitivity
  • Biogeography
  • Dinosaurs
  • Faunal turnover
  • Latitudinal climate gradients
  • Belly River Group

BMC Ecology

ISSN: 1472-6785

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