Skip to main content
Download PDF

Expression patterns of a circadian clock gene are associated with age-related polyethism in harvester ants, Pogonomyrmex occidentalis

BMC Ecology volume 9, Article number: 7 (2009) Cite this article

Abstract

Background

Recent advances in sociogenomics allow for comparative analyses of molecular mechanisms regulating the development of social behavior. In eusocial insects, one key aspect of their sociality, the division of labor, has received the most attention. Age-related polyethism, a derived form of division of labor in ants and bees where colony tasks are allocated among distinct behavioral phenotypes, has traditionally been assumed to be a product of convergent evolution. Previous work has shown that the circadian clock is associated with the development of behavior and division of labor in honeybee societies. We cloned the ortholog of the clock gene, period, from a harvester ant (Pogonomyrmex occidentalis) and examined circadian rhythms and daily activity patterns in a species that represents an evolutionary origin of eusociality independent of the honeybee.

Results

Using real time qPCR analyses, we determined that harvester ants have a daily cyclic expression of period and this rhythm is endogenous (free-running under dark-dark conditions). Cyclic expression of period is task-specific; foragers have strong daily fluctuations but nest workers inside the nest do not. These patterns correspond to differences in behavior as activity levels of foragers show a diurnal pattern while nest workers tend to exhibit continuous locomotor activity at lower levels. In addition, we found that foragers collected in the early fall (relative warm, long days) exhibit a delay in the nightly peak of period expression relative to foragers collected in the early spring (relative cold, short days).

Conclusion

The association of period mRNA expression levels with harvester ant task behaviors suggests that the development of circadian rhythms is associated with the behavioral development of ants. Thus, the circadian clock pathway may represent a conserved 'genetic toolkit' that has facilitated the parallel evolution of age-related polyethism and task allocation in social insects.

Background

Recent advances in sociogenomics, namely the ability to characterize molecular pathways in non-model organisms, provide novel opportunities to address questions concerning the evolution of social behavior in an ecological context by comparing taxonomically relevant species [1, 2]. This evo-devo approach to behavior promises to be particularly successful in understanding the most extreme form of social organization, eusociality [3]. Eusocial species exhibit cooperative brood care, overlapping generations of individuals living together, and a division of labor in which one or few individuals monopolize reproduction. The Hymenoptera, including the ants, bees, and wasps, are ideally suited for comparative studies of social behavior as species in this group display a diversity of forms of social organization and represent multiple independent evolutionary origins of eusociality [4]. Research in the behavioral genetics of social insects reveals that molecular pathways associated with behaviors in social species include behavioral genes expressed in solitary species [reviewed in [5–9]]. To date, we have little information on whether genetic mechanisms are conserved across eusocial species or whether derived social phenotypes are a product of convergent evolution driven by alternative molecular pathways.

In a eusocial insect colony, behaviors must be coordinated or synchronized among potentially thousands of individuals at any point in time. In addition to a reproductive division of labor, non-reproductive workers in highly derived eusocial species also display a division of labor among adult workers who perform specialized colony tasks [4]. The success and survival of the colony depends on a variety of tasks; some tasks occur inside the nest, such as brood care and nest construction, while others occur primarily outside the nest, such as nest maintenance, nest defense, and foraging for food.

Task allocation, the coordination of behaviors in eusocial insect colonies, often occurs without any central or hierarchical command [10]. Individuals can display plasticity in their propensity to perform these behaviors throughout their lifetime [11]. In some highly derived eusocial species, individuals display age polyethism, i.e. they progress through sequences of several tasks in a development-dependent manner [1, 4, 12]. A recent review article by Toth and Robinson [1] highlighted comparisons between honeybee and harvester ants to illustrate potential scenarios explaining the independent evolution of age polyethism in eusocial insects. Age-related polyethism in ants and bees could either arise as a result of convergent evolution of behavioral phenotypes [1] or as a result of parallel evolution of behaviors via a conserved molecular mechanism shared by a common ancestral phenotype [1, 13].

Efforts to determine the physiological and genetic bases for behavioral task development in non-reproductive eusocial workers have thus far focused on pathways involved in the development of nurse bees and foragers in honeybees [6, 14–18]. One promising candidate pathway involves the role of the circadian clock in regulating age-dependent rhythmicity [14, 16, 19, 20]. Honeybee foragers, which likely benefit from coordinating an internal clock with timing of nectar production and availability or sun-compass navigation, exhibit behavioral rhythmicity and a daily oscillation in the clock gene, period [14]. In contrast, nurse bees, which provide brood care inside the nest, exhibit arrhythmicity in locomotor behavior, and lower overall levels and weaker cyclical expression of period mRNA [14, 16]. The results from honeybees suggest a link between foraging behavior, up-regulation of the period gene, and onset of circadian rhythms [14, 16, 20]. Given that age polyethism is a derived state in eusocial insect colonies [1, 4], we ask whether foraging behavior is associated with the developmental regulation of circadian clock in the harvester ant, a species that evolved eusociality and age-related polyethism independently of bees [4, 21].

The seed-eating harvester ants, genus Pogonomyrmex, are the behavioral model for task allocation in ants [1, 10, 12, 22]. Large colonies of harvester ants (10,000–12,000 workers) inhabit the desert floors of the southwestern United States and the organization of these colonies runs, well, like clockwork. Young harvester ant workers tend to remain deep inside the nest with the single queen and perform tasks related to brood care, while older workers perform nest maintenance and patrolling tasks at the nest entrance and foraging tasks outside of the nest [12, 23]. In P. barbatus, the species best characterized for behavior, foragers have specific behavioral patterns that relate to daily temperatures and environmental conditions [12]. In summer months, they emerge from the nest early in the morning and the majority of foraging occurs during the morning hours until the sun rises and temperatures increases. By late morning, most foraging activity has ceased, although some activity occurs again in the late afternoon. During the winter months, harvester ant colonies show little activity outside of the nest. Like honeybees, harvester ant foraging activity is dependent on light and temperature, but the developmental timeline of workers is vastly different between bees (0–20 days) and ants (months – year). Thus, we are interested in whether the association of foraging behavior and circadian rhythms is conserved across diverse developmental time scales.

We cloned a harvester ant ortholog to the period gene, PoPer, from a closely related species that is easily maintained in the laboratory, Pogonomyrmex occidentalis. We analyzed the expression of PoPer in brains isolated from individual workers in field-collected colonies housed under controlled light and temperature conditions in the laboratory. We show that P. occidentalis workers display a daily oscillation in period mRNA expressed in the brain and that this oscillation is endogenous. We compare the expression patterns of nest workers with foragers and show that differences in daily expression patterns correspond to differences in locomotor activity. In addition, we show that patterns of daily oscillations in period mRNA in foragers differ across seasons.

Results

PoPerexpression in 12:12 LD cycle

In the colonies collected in the fall (F06), foragers displayed a significant oscillation in PoPer mRNA expression with levels peaking in the late evening and the lowest levels occurring in late morning hours (Figure 1; F = 3.879, df = 5,11, p = 0.049).

Figure 1
figure1

Expression of PoPer mRNA in fall foragers in LD and DD conditions. Relative expression of period mRNA in individual forager brains from experimental colonies of P. occidentalis collected in the early fall (w/SE). Colonies were entrained in controlled environmental chambers in either a 12:12 hr LD regime (light bars, n = 5 colonies) or a 24 hr DD regime (dark bars, n = 3 colonies). Bars represent the averages of colony means (four individuals sampled per colony at six time points for both light regimes; SE calculated from colonies). The diagonal stripes in the horizontal bar at base of the plot represent the actual (LD) or subjective light phase (DD) and the solid stripe represents the dark phase. All individuals were collected for analysis in dark conditions.

Full size image

A similar pattern can be seen in foragers collected in the spring (S06) with the exception that there is a shift in maximum/minimum RNA levels (Figure 2; F = 3.519, df = 5,17, p = 0.023). In S06 colonies, the peak of mRNA expression occurs at least 4 hours earlier than the fall colonies and the lowest expression levels occur at 8:00 AM. Differences in PoPer mRNA levels across time points were not significant in spring nest workers (Figure 2; F = 1.453, df = 3, 11, p = 0.298), suggesting no oscillation in period expression levels in nest workers. Nest workers were found to have significantly different PoPer mRNA levels than foragers across time points (Table 1).

Figure 2
figure2

Expression of PoPer mRNA in spring nestworkers and foragers (LD conditions). Relative expression of period mRNA in individual worker brains from experimental colonies of P. occidentalis collected in the spring (w/SE). Colonies were entrained in controlled environmental chambers in a 12:12 hr LD regime. Foragers were collected at six time points (dark bars, n = 4 colonies) and nest workers were collected at 4 time points (light bars, n = 4 colonies). Bars represent the averages of colony means with three nest workers and three foragers sampled per colony (SE calculated from colonies). The open stripe in the horizontal bar at base of the plot represents the light phase and the solid stripe represents the dark phase. All individuals were collected for analysis in dark conditions.

Full size image
Table 1 Results from two-way ANOVAs on light condition and task.
Full size table

Although foragers from different colonies do vary in PoPer expression levels, no significant differences in expression patterns were observed among colonies across time points in F06 (F = 0.836, df = 5, 183, p = 0.559) or S06 (F = 1.042, df = 4, 172, p = 0.406).

PoPerexpression in 24 DD cycle

In DD conditions, PoPer mRNA expression levels were significantly different across time points in F06 foragers (Figure 1; F = 5.75, df = 5,67, p < 0.001). Patterns of expression were not significantly different between LD and DD F06 foragers (Table 1). A similar pattern occurs in the spring – S06 foragers in DD conditions have a significant increase in evening levels of PoPer mRNA (Figure 3; F = 7.32, df = 5,18, p = 0.0007). Overall differences in PoPer expression between 24 hr DD foragers and 12:12 hr LD foragers were not significant, but there was a significant interaction between time and light regime (F = 2.85, df = 5, 34, p = 0.0297).

Figure 3
figure3

Expression of PoPer mRNA in spring foragers (DD conditions). Relative expression of period mRNA in individual forager brains from three experimental colonies of P. occidentalis collected in the spring and entrained in a 24 hr DD regime (w/SE). Bars represent the averages of colony means with three foragers sampled per colony (SE calculated from colonies). The gray stripe in the horizontal bar at base of the plot represents the subjective light phase and the solid stripe represents the dark phase in DD conditions.

Full size image

Behavioral analyses

Forager activity levels during the projected daytime were two times greater than activity levels in the projected evening (Figure 4a). Nest workers typically showed a brief peak of locomotor activity early in the projected morning and lower, variable levels of activity throughout the day and projected evening hours (Figure 4b). Significant differences in activity levels were found between nest workers and foragers (F = 32.24, df = 1, 54, p < 0.0001) and across time points (F = 13.36, df = 5, 54, p < 0.001), and a significant interaction was found between worker task and time point (F = 20.36, df = 5, 54, p < 0.0001).

Figure 4
figure4

Average locomotor activity of P. occidentalis workers in DD conditions. Average levels of locomotor activity in individual P. occidentalis workers differ by task in DD conditions. Bars represent average values of locomotor activity in two-hour increments (w/SE, n = 5 foragers (A); n = 6 nest workers (B)). The gray stripe in the horizontal bar at base of the plot represents the subjective light phase and the solid stripe represents the dark phase in DD conditions. Forager activity levels (A) during the projected daytime were two times higher than activity levels in the projected nighttime. Nest workers (B) typically showed a brief peak of locomotor activity early in the projected morning and lower, variable levels of activity throughout the day and projected evening hours.

Full size image

Discussion

The daily behavioral rhythms of harvester ant foragers suggest a role of the circadian clock in mediating foraging behavior. Here we find that the expression pattern of period mRNA in forager brains is characterized by a strong diurnal fluctuation and that this oscillation is endogenous. PoPer mRNA is present in brains of young nest workers, but overall expression levels are lower than levels seen in foragers and there is no evidence of a diurnal cycle. Locomotor activity patterns of workers correspond with expression patterns – foragers show a diurnal cycle in activity levels while young nest workers show relatively continuous locomotor activity. Observed task-specific differences in period gene expression and behavioral activity levels suggest that nest workers are arrhythmic with respect to daily light regimes but foragers experience strong circadian rhythms in behavior. Our results indicate that the age-related division of labor in ants may be associated with both changes in the expression of a clock gene and the onset of behavioral circadian rhythmicity.

The association between oscillations in period expression and foraging behavior in ants parallels the pattern seen in honeybees. In bees, period expression levels are high in foragers relative to young nurse bees and strong, consistent oscillation in per mRNA levels are typically seen in foragers [14, 16, 20]. Nurse bees show greater variation in per levels, perhaps suggesting variation in developmental changes in anticipation of foraging behavior [20]. In harvester ants, young nest workers also had highly variable expression levels of per relative to foragers. Two alternative explanations exist for the absence of significant oscillation pattern in young harvester ant nest workers – difficulty in assaying significant changes in mRNA levels due to lower overall amounts of mRNA compared to foragers, or arrhythmicity among pooled individuals (the clocks of nest workers are operational, but not synchronized to other individuals). Due to the design of real-time PCR experiments on gene expression in brains, the sampling of individuals over time is not possible. However, the finding that the signature increase in forager period mRNA levels during the dark phase is not evident in the nest workers suggests that the diurnal fluctuations in period mRNA associated with clock function are not present in young ants. Thus, a similar mechanism of developmental regulation of a clock gene in social species may extend across different developmental time scales: bees develop from nurses into foragers in approximately 7–20 days, whereas ants develop into adult foragers over the course of months.

Both honeybees and harvester ants show higher overall levels of period mRNA in foragers, suggesting that there may be a functional significance to the increase of per during behavioral maturation. The difference between tasks is small (2–5 fold) but small changes in gene expression levels have been shown to have big effects on behavior, eg. [24]. In addition, recent work on suites of genes differentially expressed in honeybee nurse and worker tasks have revealed that many genes exhibit differences within this range [6, 18]. It is not yet known whether the magnitude of differences in per mRNA levels translates into significant differences in Period protein in ants, but results from honeybees suggest that relative levels of Period protein correspond to daily fluctuations in per mRNA [25].

An important question is whether the circadian clock indeed functions to regulate behavioral maturation in social insects or whether the division of labor regulates the development of the circadian clock. Although the division of labor in ants and bees are associated with circadian plasticity and developmental regulation of per expression in ants and bees, it is difficult to directly infer functional causality because these traits are not measured in the same individuals. As molecular techniques in manipulating genomes of non-model organisms improve, direct tests of these hypotheses are necessary.

A related question that must be addressed is whether the development of clock function is associated directly with age, body size, social experience, or some other factor linked to task behavior. For example, circadian rhythms are associated with a size-dependent division of labor in bumblebees [26]. In honeybees, there is also evidence for reversals of behavioral rhythms in foragers that revert to brood care [27], suggesting that there is flexibility in systems of age polyethism [23]. In harvester ants, behavioral phenotypes are not limited to nest worker and forager tasks but include other specialized tasks both inside and outside of the nest [22]. Determining when the development of circadian rhythms and cyclic expression of period mRNA occurs in the intermediate tasks between young nest workers and old foragers will provide critical clues to understanding whether circadian rhythms are limited to foragers, involved in behavioral maturation of ants and/or due to behavioral experience outside of the nest.

Finally, our results suggest a link between seasonal variation in behavioral patterns of harvester ants and circadian clock gene expression. Circadian clock pathways respond to both light and temperature and it is thought that these two factors work in coordination to allow the clock mechanism to adapt to seasonal changes in photoperiod and temperature [28]. In Drosophila, per is rhythmic under light and temperature entrainment, but at low temperatures, there is a phase difference at transcript level (but not protein) level. An advance in per expression coupled with temperature sensitive splicing at the 3' UTR of the gene is thought to enable the fly to seasonally adapt to cold, short days perhaps by increasing activity levels earlier in the day to combat early nightfall [29, 30]. Seasonal variation in behavioral rhythms (free running period or FRP) exist in other organisms, including honeybees from colonies sampled in different months of the year [31]. Harvester ant colonies experience a seasonal dormancy during the colder months of year, with very low or no external foraging activity outside of the nests. In this study, we see an advance in the timing of daily per upregulation in foragers collected in early spring (cold, short days) relative to foragers collected in early fall (long, warm days). Thus, our results are consistent with results from studies of seasonal variation in per expression patterns in fruitflies [29, 30]. The advance in timing of high per expression levels in harvester ants in the spring relative to early fall may function to increase the activity of foragers earlier in the day when more light is available and temperatures are less extreme. Further work will elucidate whether the advance in per expression is directly related to seasonal behavioral patterns of foragers in field colonies and what mechanism drives the shift in the expression cycle of per mRNA in ants.

Conclusion

The circadian clock pathway has emerged as an intriguing potential mechanism involved in the division of labor in social insects. The conservation of circadian clock functions across diverse taxa contrasts sharply with new evidence of the surprising degree of divergence in the underlying molecular pathways, especially in insects [32, 33]. Particularly exciting is the recent discovery that features of honeybee circadian clocks resemble mammalian clocks more than Drosophila clocks [33]. Given the potential diversity of pathways, the association of diurnal or nocturnal locomotor activity with the cyclical expression pattern of the period gene has remained one of the most conserved components of the circadian mechanism. Our results and future studies will help elucidate how the ancient system of molecular clock function has been co-opted for similar functions in social insects, providing a fascinating link between chronobiology and sociobiology.

Methods

Ants and sampling

Laboratory colonies of harvester ants (Pogonomyrmex occidentalis) were established by collecting field colonies in Hurricane, Utah in February/March 2006 (S06, 4 colonies) and September 2006 (F06, 4 colonies). Colonies were transferred to individual plexiglass nest boxes (15 cm wide × 30 cm long × 10 cm high) connected to open-air foraging arenas (25 cm W × 60 cm L × 15 cm H) by short Tygon tubing (2 cm diameter × 10 cm long). Nest box floors had a 3 cm layer of plaster with built-in irrigation system to keep colonies moist. Nest boxes were covered in double layers of red cellophane and had removable lids for ease of observation and collection. Colonies were kept under stable conditions of light, temperature and humidity in controlled environmental chambers (lights on in LD conditions at 6 AM). Laboratory colonies did not contain queens, but did contain some larvae. For both spring and fall experiments, original colonies collected in the field were divided into two queenless laboratory colonies of at least 400 workers that were then subjected to the different entrainment regimes. In the S06 study, each of four colonies were divided into two laboratory colonies and entrained in either 12 h:12 h light-dark (LD) conditions (4 colonies) or 24 h dark-dark (DD) conditions (4 colonies) at 15/12.5°C with ~70% relative humidity. Collection of S06 time point samples occurred in DD conditions at a constant temperature of 14.5°C. In the F06 study, each of five colonies were divided into two laboratory colonies and entrained in either 12 h:12 h light-dark (LD) conditions (5 colonies) or 24 h dark (DD) conditions (3 colonies only) at 19/16°C with ~80% relative humidity. Collection of F06 time point samples occurred in DD conditions at constant temperature of 19°C.

Foragers were identified as ants that were observed on the food in the foraging arena and were labeled using a dot of acrylic paint during the day. Nest workers were identified as ants that remained in the covered nest box and were never observed in the foraging arena. Exact ages of the foragers and nest workers were not known, but nest workers are typically younger than foragers [23, 34, 35]. We expect there may be seasonal differences in the age of nest workers – in the spring, a burst of brood production [34] yields nest workers that are likely to be younger than their fall counterparts. We do not expect seasonal differences in the ages of foragers. Thus, comparisons between nest workers and foragers were conducted in the spring.

We collected multiple ants (6–10) at each timepoint and reported sample sizes are the RNA samples successfully extracted from these collections (either 3 or 4 individuals per colony and 3–5 colonies per time point, depending on season). In the S06 study, RNA samples from three ants were collected from each colony per time point of collection; in the F06 study, RNA samples from four ants were collected from each colony. All sampling was done using dim red light in dark conditions. Ants were collected from each colony at six time points: 4:00, 8:00, 12:00, 16:00, 20:00, and 24:00 hours. Individual ants were immediately immersed in liquid nitrogen and stored at -70°C until dissection. Brain dissections were performed in 50 uL 1× PBS and 5 uL RNAlater® under a dissecting microscope. RNA isolation was done using the RNeasy Plus Mini Kit according to manufacturer's instructions (Qiagen) and isolated RNA was stored at -70°C. In the spring study, RNA was purified from 144 individual brain dissections, 432 cDNA reactions were amplified and 864 qPCR reactions were analyzed for the two genes. In the fall study, RNA was purified from 192 individual brain dissections, cDNA was amplified in triplicate for each RNA sample (n = 576) and 1152 qPCR reactions were analyzed.

Quantitative real-time PCR

Conserved blocks of amino acid sequence from known period genes in Drosophila melanogaster, Apis mellifera, Bombus ardens, and Formica japonica were identified with the CODEHOP program [36, 37]. Degenerate primers (see Additional file 1) were designed to amplify the ant ortholog to period (ABI Big-Dye Sequencing technology on an ABI 377 instrument) from genomic DNA and cDNA. Harvester ant-specific primers were designed from exon-coding regions to amplify a 90 bp region for qPCR analyses. Percent nucleotide/amino acid similarity to known eusocial insect per sequences was estimated using MacVector® (F. japonica, 92/93%; A. mellifera, 76/82%; B. ardens, 74/78%). Real time primers (PoPerF: 5'-TCCTTCAGGTCGAAGCCGT-3'; PoPerR:5'-TGATAAAGGACGACCACTCGG-3') and Taqman probe® (PoPerT:5'DFAM-CAGATTCGCCGTGCAGAACGGG-3'DTAM) were designed using Primer Express® software (ABI).

cDNA was synthesized from extracted total RNA preps using ABI TaqMan Reverse Transcriptase reagents and an oligo-dT primer. Reactions were performed in triplicate for each individual brain. All reactions were run at 25°C for 10 minutes, 48°C for 30 minutes, 95°C for 5 minutes, and then stored at -80°C until quantitative PCR. For each cDNA replicate, expression of PoPer was assayed on an ABI 7900 HT instrument using ABI Taqman Gold reagents. To standardize period expression, an ant homolog of the RNA polymerase II 512 kD (PoRPII) subunit was used as a control [38] for each cDNA replicate (see Additional file 1). Real-time PCR reactions for Poper and PoRPII were performed under the following conditions: 2 min 50°C, 10 min 95°C, 15 sec 95°C, 1 min 58°C, for 45 cycles. Data was analyzed using SDS 2.1 software and quantification of relative mRNA levels was calculated using the ΔΔCt method. Three (S06) or four (F06) individual brains were pooled to calculate a colony value per time point. Colony values were averaged at each time point for comparisons across a 24 hr period. Differences in relative levels of PoPer expression over time were analyzed for each task and condition using one-way analysis of variance tests. Differences in expression levels between foragers in LD and DD conditions (S06 & F06) and between foragers and nest workers (S06 only) were analyzed using two-way analysis of variance tests. Differences between colonies (S06 & F06) were analyzed using repeated measures analysis of variance tests. All analyses were performed using SPSS software.

Analysis of P. occidentalislocomotor activity

We observed daily activity patterns of individual nest workers and foragers from colonies of P. occidentalis in the absence of social interactions and light stimulus. Colonies were collected in April and entrained for five days in 12:12 LD conditions. Individual nest workers (n = 6) and foragers (n = 5) were removed from colonies and placed in Petri dishes with a simple line bisecting the bottom of the dish. Ants were placed in separate environmental chambers under constant temperature (18°C) and humidity (80%) conditions with a DD light regime for 24 hours. On the second day in DD conditions, ants were videotaped using infrared technology (Sony Handycam) over 24 hour periods and activity was measured via manual inspection as the number of times an individual crossed the line per hour by observers who were blind to the experimental conditions. Activity levels of individuals were averaged over two-hour intervals for each task (Figure 4). In addition, four-hour 'bins' were constructed to correspond to the six time points in the RNA analyses by averaging values per individual (eg. 6 AM-10 AM values represented the 8 AM time point). Differences between nest worker and forager daily activity patterns over the six time points were analyzed with a two-way repeated measures ANOVA.

References

  1. 1.

    Toth AL, Robinson GE: Evo-devo and the evolution of social behavior. Trends Genet. 2007, 23 (7): 334-341. 10.1016/j.tig.2007.05.001.

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Robinson GE: Development. Sociogenomics takes flight. Science. 2002, 297 (5579): 204-205. 10.1126/science.1074493.

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Toth AL, Varala K, Newman TC, Miguez FE, Hutchison SK, Willoughby DA, Simons JF, Egholm M, Hunt JH, Hudson ME, Robinson GE: Wasp gene expression supports an evolutionary link between maternal behavior and eusociality. Science. 2002, 318 (5849): 441-444. 10.1126/science.1146647.

    Article  Google Scholar 

  4. 4.

    Wilson EO: The insect societies. 1971, Cambridge, Mass.: Belknap Press of Harvard University Press

    Google Scholar 

  5. 5.

    Robinson GE, Grozinger CM, Whitfield CW: Sociogenomics: social life in molecular terms. Nature Reviews Genetics. 2005, 6 (4): 257-271. 10.1038/nrg1575.

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Whitfield CW, Ben-Shahar Y, Brillet C, Leoncini I, Crauser D, Leconte Y, Rodriguez-Zas S, Robinson GE: Genomic dissection of behavioral maturation in the honey bee. Proc Natl Acad Sci USA. 2006, 103 (44): 16068-16075. 10.1073/pnas.0606909103.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  7. 7.

    Sumner S, Pereboom JJ, Jordan WC: Differential gene expression and phenotypic plasticity in behavioural castes of the primitively eusocial wasp, Polistes canadensis. Proc Biol Sci. 2006, 273 (1582): 19-26. 10.1098/rspb.2005.3291.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  8. 8.

    Ingram KK, Oefner P, Gordon DM: Task-specific expressionof the foraging gene in harvester ants. Mol Ecol. 2005, 14 (3): 813-818. 10.1111/j.1365-294X.2005.02450.x.

    CAS  Article  PubMed  Google Scholar 

  9. 9.

    Hoffman EA, Goodisman MA: Gene expression and the evolution of phenotypic diversity in social wasps. BMC Biol. 2007, 5: 23-10.1186/1741-7007-5-23.

    PubMed Central  Article  PubMed  Google Scholar 

  10. 10.

    Gordon DM: The organization of work in social insect colonies. Nature. 1996, 380 (6570): 121-124. 10.1038/380121a0.

    CAS  Article  Google Scholar 

  11. 11.

    Michener CD: The social behavior of the bees: a comparative study. 1974, Cambridge, Mass.: Belknap Press of Harvard University Press

    Google Scholar 

  12. 12.

    Gordon D: Ants at work: how an insect society is organized. 1999, New York: Free Press

    Google Scholar 

  13. 13.

    Turillazzi S, West-Eberhard MJ: Natural history andevolution of paper-wasps. 1996, Oxford England; New York: Oxford University Press

    Google Scholar 

  14. 14.

    Bloch G, Toma DP, Robinson GE: Behavioral rhythmicity, age, division of labor and period expression in the honey bee brain. J Biol Rhythms. 2001, 16 (5): 444-456. 10.1177/074873001129002123.

    CAS  Article  PubMed  Google Scholar 

  15. 15.

    Ben-Shahar Y: The foraging gene, behavioral plasticity, and honeybee division of labor. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2005, 191 (11): 987-994. 10.1007/s00359-005-0025-1.

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Toma DP, Bloch G, Moore D, Robinson GE: Changes in period mRNA levels in the brain and division of labor in honey bee colonies. Proc Natl Acad Sci USA. 2000, 97 (12): 6914-6919. 10.1073/pnas.97.12.6914.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  17. 17.

    Ben-Shahar Y, Robichon A, Sokolowski MB, Robinson GE: Influence of gene action across different time scales on behavior. Science. 2002, 296 (5568): 741-744. 10.1126/science.1069911.

    CAS  Article  PubMed  Google Scholar 

  18. 18.

    Whitfield CW, Cziko AM, Robinson GE: Gene expression profiles in the brain predict behavior in individual honey bees. Science. 2003, 302 (5643): 296-299. 10.1126/science.1086807.

    CAS  Article  PubMed  Google Scholar 

  19. 19.

    Shemesh Y, Cohen M, Bloch G: Natural plasticity in circadian rhythms is mediated by reorganization in the molecular clockwork in honeybees. FASEB J. 2007, 21 (10): 2304-2311. 10.1096/fj.06-8032com.

    CAS  Article  PubMed  Google Scholar 

  20. 20.

    Bloch G, Rubinstein CD, Robinson GE: Period Expression in the Honey Bee Brain is Developmentally Regulated and Not Affected by Light, Flight Experience, Or Colony Type. Insect Biochem Mol Biol. 2004, 34 (9): 879-891. 10.1016/j.ibmb.2004.05.004.

    CAS  Article  PubMed  Google Scholar 

  21. 21.

    Keller L, Genoud M: Extraordinary lifespans in ants: a test of evolutionary theories of ageing. Nature. 1997, 389 (6654): 958-960. 10.1038/40130.

    CAS  Article  Google Scholar 

  22. 22.

    Gordon DM: The dynamics of the daily round of the harvester ant colony (Pogonomyrmex barbatus). Anim Behav. 1986, 34 (5): 1402-1419. 10.1016/S0003-3472(86)80211-1.

    Article  Google Scholar 

  23. 23.

    Gordon DM, Chu J, Lillie A, Tissot M, Pinter N: Variation in the transition from inside to outside work in the red harvester ant Pogonomyrmex barbatus. Insectes Soc. 2005, 52 (3): 212-217. 10.1007/s00040-004-0796-3.

    Article  Google Scholar 

  24. 24.

    Greenspan RJ: A kinder, gentler genetic analysis of behavior: dissection gives way to modulation. Curr Opin Neurobiol. 1997, 7 (6): 805-811. 10.1016/S0959-4388(97)80139-0.

    CAS  Article  PubMed  Google Scholar 

  25. 25.

    Bloch G, Solomon SM, Robinson GE, Fahrbach SE: Patterns of PERIOD and pigment-dispersing hormone immunoreactivity in the brain of the European honeybee (Apis mellifera): age- and time-related plasticity. J Comp Neurol. 2003, 464 (3): 269-284. 10.1002/cne.10778.

    CAS  Article  PubMed  Google Scholar 

  26. 26.

    Yerushalmi S, Bodenhaimer S, Bloch G: Developmentally determined attenuation in circadian rhythms links chronobiology to social organization in bees. J Exp Biol. 2006, 209 (Pt 6): 1044-1051. 10.1242/jeb.02125.

    Article  PubMed  Google Scholar 

  27. 27.

    Bloch G, Robinson GE: Reversal of honeybee behavioural rhythms. Nature. 2001, 410: 1048-10.1038/35074183.

    CAS  Article  PubMed  Google Scholar 

  28. 28.

    Boothroyd CE, Wijnen H, Naef F, Saez L, Young MW: Integration of light and temperature in the regulation of circadian gene expression in Drosophila. PLoS Genet. 2007, 3 (4): e54-10.1371/journal.pgen.0030054.

    PubMed Central  Article  PubMed  Google Scholar 

  29. 29.

    Majercak J, Chen WF, Edery I: Splicing of the period gene 3'-terminal intron is regulated by light, circadian clock factors, and phospholipase C. Mol Cell Biol. 2004, 24 (8): 3359-3372. 10.1128/MCB.24.8.3359-3372.2004.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  30. 30.

    Chen WF, Low KH, Lim C, Edery I: Thermosensitive splicing of a clock gene and seasonal adaptation. Cold Spring Harb Symp Quant Biol. 2007, 72: 599-606. 10.1101/sqb.2007.72.021.

    CAS  Article  PubMed  Google Scholar 

  31. 31.

    Bloch G, Shemesh Y, Robinson G: Seasonal and task-related variation in free running activity rhythms in honey bees (Apis mellifera). Ins Soc. 2006, 53: 115-118. 10.1007/s00040-005-0844-7.

    Article  Google Scholar 

  32. 32.

    Yuan Q, Metterville D, Briscoe AD, Reppert SM: Insect cryptochromes: gene duplication and loss define diverse ways to construct insect circadian clocks. Mol Biol Evol. 2007, 24 (4): 948-955. 10.1093/molbev/msm011.

    CAS  Article  PubMed  Google Scholar 

  33. 33.

    Rubin EB, Shemesh Y, Cohen M, Elgavish S, Robertson HM, Bloch G: Molecular and phylogenetic analyses reveal mammalian-like clockwork in the honey bee (Apis mellifera) and shed new light on the molecular evolution of the circadian clock. Genome Res. 2006, 16 (11): 1352-1365. 10.1101/gr.5094806.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  34. 34.

    MacKay WP: A comparison of the nest phenologies of three species of Pogonomyrmex harvester ants (HYMENOPTERA:FORMICIDAE). Psyche. 1981, 88 (1–2): 25-74. 10.1155/1981/78635.

    Article  Google Scholar 

  35. 35.

    Gordon DM, Holldobler B: Worker longevity in harvester ants (Pogonomyrmex). Psyche. 1987, 94: 341-346. 10.1155/1987/63930.

    Article  Google Scholar 

  36. 36.

    Rose T, Henikoff J, Henikoff S: CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design. Nucl Acids Res. 2003, 31 (13): 3763-3766. 10.1093/nar/gkg524.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  37. 37.

    Rose T, Schultz E, Henikoff J, Pietrokovski S, McCallum C, Henikoff S: Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences. Nucl Acids Res. 1998, 26 (7): 1628-1635. 10.1093/nar/26.7.1628.

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  38. 38.

    Radonic A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A: Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun. 2004, 313 (4): 856-862. 10.1016/j.bbrc.2003.11.177.

    CAS  Article  PubMed  Google Scholar 

Download references

Acknowledgements

We thank J. Abrahams, S. Merrill, S. Dolan, and L. Janson for assistance with the molecular and behavioral data, A. Fawcett for assistance with field collections, D. McHugh and N. Shult for comments on an earlier version of this manuscript, and the National Institutes of Health (NIGMS-NRSA to KI) and the US National Science Foundation (NSF-MRI to KI) and Colgate University for providing the funds necessary to conduct this research.

Author information

Affiliations

  1. Department of Biology, Colgate University, Hamilton, New York, USA

    Krista K Ingram, Scott Krummey & Michelle LeRoux

Authors
  1. Krista K Ingram
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Scott Krummey
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michelle LeRoux
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Krista K Ingram.

Additional information

Authors' contributions

KI conceived of the study, participated in molecular genetic work and statistical analyses, and drafted the manuscript. SK and ML participated in the design of the study, the molecular genetic work and statistical analyses. All authors read and approved the final manuscript.

Electronic supplementary material

Authors’ original submitted files for images

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Ingram, K.K., Krummey, S. & LeRoux, M. Expression patterns of a circadian clock gene are associated with age-related polyethism in harvester ants, Pogonomyrmex occidentalis. BMC Ecol 9, 7 (2009). https://doi.org/10.1186/1472-6785-9-7

Download citation

Keywords

  • Circadian Clock
  • Brood Care
  • Eusocial Insect
  • Daily Oscillation
  • Daily Activity Pattern

BMC Ecology

ISSN: 1472-6785

Contact us