Fig. 1

Tranylcypromine treatment of Drosophila Schneider’s S2 cells raised methylation of histone H3 at lysine 4 (H3K4). S2 cells were grown in standard medium (control) or in the presence of either Tranylcypromine dissolved in water (Tranyl in water), or Tranylcypromine dissolved in DMSO (Tranyl in DMSO). Cells were lysed and sonified in SDS sample buffer, separated on 15% SDS-PAGE, and histone H3 and di-and trimethylated histone H3K4 detected by fluorescent secondary antibodies. a Representative Western blot showing detection of histone H3 (red) and histone H3 di-and trimethylated at lysine 4 (H3K4me2/3; green). b Ratio of the quantity of H3K4me2/3 related to H3 and calculated as the fold change of the control. Control (3 biological replicates, n = 9 × loaded), Tranylcypromine in water (3 biological replicates, n = 7 × loaded), Tranylcypromine in DMSO (3 biological replicates, n = 8 × loaded). Data are normally distributed. Significant differences to the control according to Student’s T test (one-tailed, homoscedastic): Tranylcypromine in water p*, Tranylcypromine in DMSO p**