Table 1 Analytical method validation characteristics for the Nicotiana glauca MeOH extract
From: An essay on ecosystem availability of Nicotiana glauca graham alkaloids: the honeybees case study
Compound | Regression equation* | Regression coefficient (R2) | LOD (ng/g) | LOQ (ng/g) | ME (%) | Recovery ± RSD % (n = 3) | Intra-d-precision (RSD % n = 3) | Inter-d-precision (RSD % n = 3) | ||
|---|---|---|---|---|---|---|---|---|---|---|
50Â ng/g | 200Â ng/g | 1000Â ng/g | 200Â ng/g | 200Â ng/g | ||||||
Anabasine | y = 6670049.8x − 37750.2 | 0.9970 | 19 | 58 | 2.9 | 81 ± 7 | 87 ± 15 | 83 ± 8 | 3.8 | 5.1 |
Nicotine | y = 1451011x + 14365.6 | 0.9997 | 26 | 79 | 1.9 | 78 ± 10 | 83 ± 14 | 93 ± 9 | 1.7 | 6.3 |
Anatabine | y = 4835017.3 + 251.2 | 0.9995 | 13 | 39 | 0.7 | 91 ± 15 | 87 ± 9 | 85 ± 12 | 4.7 | 6.2 |
Nornicotine | y = 41448304x − 299690.9 | 0.9997 | 10 | 30 | 5.8 | 76 ± 8 | 82 ± 14 | 87 ± 10 | 2.5 | 4.3 |
Myosmine | y = 2387692.2x − 9948.8 | 0.9947 | 11 | 34 | 4.2 | 80 ± 8 | 76 ± 7 | 82 ± 6 | 6.5 | 7.3 |
Scopoletin | y = 1839928.8x − 3670.4 | 0.9999 | 24 | 72 | −1.9 | 85 ± 10 | 91 ± 10 | 90 ± 12 | 1.7 | 6.1 |
Cotinine | y = 12933154x − 44736.5 | 0.9979 | 11.3 | 33.4 | 5.7 | 75 ± 5 | 80 ± 8 | 94 ± 12 | 3.8 | 3.2 |