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Table 2 Specificity of the NemF primer

From: High-throughput sequencing of nematode communities from total soil DNA extractions

NemF

G

G

G

G

A

A

G

T

A

T

G

G

T

T

G

C

A

A

A

Nematode

100

100

100

100

81

100

100

100

100

100

100

100

100

100

100

100

100

100

100

Fungi

.

.

.

.

G

.

.

.

.

.

.

.

.

C

.

.

.

.

G

Plantae

.

.

.

.

G

.

.

.

.

.

.

.

.

C

.

.

.

.

G

Metazoan

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

  1. The first row below the NemF sequence highlights the % conservation at each nucleotide site. The other rows highlight the consensus sequence in fungi, plantae and metazoans respectively; conserved positions are shown as a dot.
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BMC Ecology

ISSN: 1472-6785

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