Figure 1

Discrimination of Microbotryum genotypes used in experimental within-host competition. Pathogen species were discriminated by colony morphology resulting from teliospore germination and growth on water agar after 72 hours at room temperature; Microbotryum silenes-inflatae (A) produced small colonies, while colonies of Microbotryum lagerheimii (C) contained many sporidia and often produced an infectious hypha. Within-species discrimination (B and D) used variation in PCR products using microsatellite primers (detailed in the Methods section).