Table 3 Feature overview of different image analysis software. Comparison of 2 recent published image analysis programs COMSTAT and ISA-3D with PHLIP. Feature information of COMSTAT [24, 26] and ISA-3D [38] was gathered from their reference publications (* data not published, ** data not available).
COMSTAT | ISA-3D | PHLIP | |
|---|---|---|---|
Operating systems | Linux, Windows | Windows 2000/XP* | Linux, OSX, Windows |
Requirements: | Matlab license + toolboxes | Matlab license + toolboxes | Matlab license only |
Reference | [24, 26] | [54] | |
Availability | Obtained free upon request | Must attend workshop (1500$) | Free downloadable (open source) |
Multi-channel capability | No | No | Yes |
GUI | No | Yes* | Yes |
CLSM support | - Leica -COMSTAT | ** | - Leica - COMSTAT - PHLIP-ML (universal converter) |
Preprocessing | None | - Image inversion | - Image inversion - Carrier adjustment - X- section resolution |
Automatic thresholding | Yes | Yes | Yes (Otsu) |
Noise reduction: | Connected volume filtration | ** | Connected volume filtration |
Image analysis parameters: | - Single channel (10) | -Single channel (20) | - Single channel (6) - Two channel (2) - All channel (5) |
Result output | - Text format | - Text format | - HTML format - XML format (PHLIP-ML) - Text-format |
Source code availability | Not available | Not available | Available (open source project) |