Table 4 Marker genes were used to trace pollen transfer from transformed to wild-type plants with intact anthers. A hand-pollination test was used to evaluate the effect of direct transfer on seed set. Moths (M. sexta) visitedtwo arrays (1 and 2) of wild-type and transformed plants but were excluded from the third array.
Treatment | Wild-type plants in treatment | Wild-type plants setting seed | Plants with transformed offspring | Seeds per capsule (Mean ± SE) | Transformed offspring per capsule (Mean ± SE) |
|---|---|---|---|---|---|
Hand-pollination | 8 | 8 | 8 | 154.2 ± 8.0 | 3.2 ± 1.3 |
Array 1 | 12 | 9 | 7 | 124.6 ± 11.8 | 2.4 ± 0.9 |
Array 2 | 1 | 1 | 1 | 148.5 ± 15.2 | 3.5 ± 1.5 |
Array 3 | 8 | 7 | 0 | 105.6 ± 7.2 | 0 |