Table 2 Mixtures of transformed and wild-type pollen were applied to the stigmas of emasculated flowers at the Hwy 91 field site. In the first experiment (using DI pollen), there was no significant relationship between the concentration of transformed pollen and the number of GUS-positive seedlings. This result may reflect the low rate of seed set and thus small sample size. In the second experiment (Hwy 91 pollen), there was a significant positive relationship (Spearman Rank Correlation: rs = 0.975, p < 0.05).
Treatment | Number of plants that set seed | Average number of viable seeds per capsule (± s.e.) | Average number of GUS + seedlings per capsule (± s.e.) |
|---|---|---|---|
Pollen from DI site mixed with transformed pollen (n = 25 replicate plants) | |||
100% DI | 3 | 24.3 ± 10.0 | 0 |
DI: tr 9:1 | 6 | 24.2 ± 16.1 | 0.17 ± 0.37 |
DI: tr 1:1 | 3 | 82 ± 20 | 5.0 ± 4.5 |
DI: tr 1:9 | 6 | 12.3 ± 13.2 | 1.5 1.8 |
100% transformed | 3 | 20 ± 14.8 | 1.0 ± 1.7 |
Pollen from Hwy 91 site mixed with transformed pollen (n = 20 replicate plants) | |||
100% 91 | 9 | 43.2 ± 23.6 | 0 |
91: tr 9:1 | 4 | 29.3 ± 26.8 | 1.3 ± 1.3 |
91: tr 1:1 | 9 | 50 ± 33.6 | 3.1 ± 3.2 |
91: tr 1:9 | 11 | 48.1 ± 33.2 | 3.1 ± 4.0 |
100% transformed | 9 | 17.3 ± 17.0 | 4.1 ± 5.4 |