Table 2 Stability ranking of candidate reference gene expression by two different algorithms, calculated for sampled subpopulations
GeNorm | NormFinder | ||||||
|---|---|---|---|---|---|---|---|
Rank | Gene | M | Rank | Gene | Stability | ||
Puerto Montt | N. magellanica, intertidal | 1st + 2nd | Grp78 + Actin | 1.163 | 1st | Grp78 | 0.38 |
+3rd | HIF | 1.316 | 2nd | HIF | 0.63 | ||
best pair | Grp78 + Actin | 0.388 | |||||
Punta Arenas | N. magellanica, intertidal | 1st + 2nd | Grp78 + HIF | 0.89 | 1st | Grp78 | 0.297 |
2nd | HIF | 0.494 | |||||
+3rd | H3 | 1.15 | best pair | Grp + HIF | 0.33 | ||
N. dearauta, sublittoral | 1st + 2nd | Grp78 + HIF | 0.86 | 1st | Grp78 | 0.14 | |
2nd | HIF | 0.29 | |||||
+3rd | Actin | 1.06 | best pair | Grp78 + HIF | 0.163 | ||
Potter Cove | N. concinna, intertidal | 1st + 2nd | HIF + H3 | 0.869 | 1st | Grp78 | 0.198 |
2nd | HIF | 0.211 | |||||
+3rd | Grp78 | 0.929 | 3rd | H3 | 0.285 | ||
best pair | Grp78 + HIF | 0.158 | |||||
N. concinna, sublittoral | 1st + 2nd | HIF + H3 | 0.636 | 1st | H3 | 0.22 | |
2nd | HIF | 0.227 | |||||
+3rd | Grp78 | 0.848 | 3rd | Grp78 | 0.3 | ||
best pair | HIF + H3 | 0.169 | |||||
All controls (0h) | Nacella | 1st + 2nd | Grp78 + Actin | 0.94 | 1st | Grp78 | 0.573 |
2nd | Actin | 0.62 | |||||
+3rd | H3 | 1.388 | 3rd | H3 | 0.704 | ||
best pair | Grp78 + HIF | 0.486 | |||||