Temporal response of the tiger salamander (Ambystoma tigrinum) to 3,000 years of climatic variation
© Bruzgul et al; licensee BioMed Central Ltd. 2005
Received: 11 May 2005
Accepted: 13 September 2005
Published: 13 September 2005
Amphibians are sensitive indicators of environmental conditions and show measurable responses, such as changes in phenology, abundance and range limits to local changes in precipitation and temperature regimes. Amphibians offer unique opportunities to study the important ecological and evolutionary implications of responses in life history characteristics to climatic change. We analyzed a late-Holocene fossil record of the Tiger Salamander (Ambystoma tigrinum) for evidence of population-level changes in body size and paedomorphosis to climatic change over the last 3000 years.
We found a significant difference in body size index between paedomorphic and metamorphic individuals during the time interval dominated by the Medieval Warm Period. There is a consistent ratio of paedomorphic to metamorphic specimens through the entire 3000 years, demonstrating that not all life history characteristics of the population were significantly altered by changes in climate on this timescale.
The fossil record of Ambystoma tigrinum we used spans an ecologically relevant timescale appropriate for understanding population and community response to projected climatic change. The population-level responses we documented are concordant with expectations based on modern environmental studies, and yield insight into population-level patterns across hundreds of generations, especially the independence of different life history characteristics. These conclusions lead us to offer general predictions about the future response of this species based on likely scenarios of climatic warming in the Rocky Mountain region.
The global mean temperature has risen 0.6°C in the last 100 years and is projected to increase between 1.4°C and 5.8°C over the next 100 years . This rate of increase (in temperature per century) is greater than any measured in the climate record of at least the last 100,000 years [2–4]. Several types of response to this rapid climatic change have been proposed for vertebrates, including range shifts to track resource changes, local adaptation to new resources, local extinctions, and developmental plasticity [5, 6]. Although these changes are difficult to observe on a seasonal or annual time scale, empirical evidence from long-term field studies and historical records exists for range shifts and phenological shifts, especially earlier breeding events (see [4, 5, 7, 8]). Clearly, long-term monitoring and historic data sets are critical to identification of meaningful biological responses to climatic change [9, 10]. However, such information is scarce, with quality ecological records confined to only the last 100 years, and often far less . One unique way to address these problems is by using biologically relevant time series data provided by the fossil record. An ideal fossil record would span the last few hundred to few thousand years in order to detect changes over tens to hundreds of generations. Such a record would provide a temporal scale long enough to identify significant trends and enough resolution to reveal ecologically relevant population responses. Ultimately, this type of data will provide information fundamental for predictions of response to climatic change over the next century.
Amphibians are often considered sensitive indicators of environmental conditions [12–16]. Many amphibian species have a naturally complex life cycle that involves both aquatic and terrestrial environments, directly exposing individuals to changes in environmental temperature and moisture over a single lifetime. Amphibian skin and eggs are highly permeable, which makes them sensitive to small changes in chemicals in the environment . Furthermore, they are ectothermic, enabling differences in temperature to have direct effects on amphibian body temperature and metabolic processes [13, 18]. Thus, projected global climatic change is expected to have significant effects on amphibian communities [18, 19], but these effects have not yet been specified. Our study system provides the data necessary to initiate such predictions.
Lamar Cave is an exceptionally rich paleontological site located in Yellowstone National Park (YNP), Wyoming, USA . The fossils are primarily from a wood rat midden collection of raptor pellets and carnivore scats. The excavated fossil collection contains tens of thousands of bones encompassing mammals, amphibians, birds, reptiles, and fish, and spans the last 3300 years . Recent investigations of the small mammal record of Lamar Cave show morphological and genetic responses that correlate well with past climatic events in the area [22, 23]. Lamar Cave also contains thousands of amphibian bones.
In particular, the most abundant amphibian in Lamar Cave, the tiger salamander (Ambystoma tigrinum) is an excellent species with which to study population level responses to climatic change. Many studies show that Ambystoma demonstrates developmental growth plasticity in response to environmental conditions (e.g. [24–27]): fossils record this as changes in mean body size. In addition, Ambystoma is able to exploit alternate life histories in response to different environmental conditions [26, 28–31]. Ambystoma may undergo an aquatic larval stage and then metamorphose into terrestrial adults or alternately, be paedomorphic, i.e., achieve sexual maturity while remaining aquatic and retaining larval morphological features. Ambystoma tigrinum is unusual in that it retains the ability to metamorphose facultatively even after years in the aquatic form . The different life histories are detectable in diagnostic characters preserved in the fossil record [32, 33] of populations represented in Lamar Cave. Thus, the cave records the potential effects of climatic change on several life history characteristics of A. tigrinum, and is documented here for the first time at this important temporal scale.
Paedomorphosis in modern A. tigrinum depends on various environmental stimuli [24, 34]. Several biotic factors influence paedomorphosis and metamorphosis including thyroid hormone levels, larval density, and competition with fish [24, 28, 30, 31, 35, 36]. Although important, these biotic factors are often overwhelmed by the influence of temperature and moisture. Populations in cold environments show high frequency of paedomorphosis  and are often large in size . Populations found in ponds of intermediate temperature often contain both paedomorphs and metamorphosing individuals. Likelihood of paedomorphosis is also closely linked to pond permanence, or perceived pond permanence [24, 28, 29, 37, 38]. Pond permanence is directly related to available moisture in the environment in the form of humidity, precipitation, or ground water.
Given the environmental forces that drive body size and metamorphic changes in A. tigrinum, we used a late-Holocene fossil record to track these traits through the last 3000 years. We analyzed trends within the context of known climatic change, and attempted to distinguish patterns of response that represent specific climatic periods through this time.
NISP and MNI of Ambystoma tigrinum fossils by time interval. The difference between the total MNI and the paedomorph plus terrestrial MNI is due to young larval MNI.
An assumption made in interpreting the fossil record is that the specimens are representative of the sample population. Another caveat is that the common characteristics of the population also will occur in the fossil record if there are no effective sampling biases . With this in mind, we expect changes in characteristics of the fossil specimens to represent changes in the sample population. Much of the fossil record for A. tigrinum through the last 3000 years indicates relatively conservative changes in population trends in morphotype, but nevertheless paedomorphic and metamorphic individuals are found throughout the deposit. Because there were no absolute shifts to a single life history strategy (i.e. paedomorphosis) within the sampled population over a relatively long ecological time period, it is likely that the two distinct life history strategies were maintained during short-term climatic fluctuations of the late Holocene. Thus, we conclude according to this case study that although change in frequency of paedomorphosis in a population occurs in relation to different environmental conditions over one generation, on the time scale of 100 to 1000s of years, if there is short-term climate variability little overall change would be expected in a population, even with coarsely distinguished climatic events.
Our results provide an opportunity to determine how the local population of A. tigrinum responded to the largest climatic anomaly in the Yellowstone region over the last 3000 years, the Medieval Warm Period (MWP) . The MWP in Yellowstone is characterized by a warm and dry climate  and occurred approximately 1150 to 650 ybp . These conditions suggest low pond permanence, or rapid drying, which could lead to shallower ponds that provide less protection from predators. The several ponds in the vicinity of Lamar Cave are all fishless glacial kettles with approximately the same water depth. Total A. tigrinum fossil abundance over the last 3,000 years shows a marked increase during Interval C, which corresponds to the MWP. Due to the deposition of this fossil assembly, fossil abundances reflect an overall increase in salamander abundance, an increase in successful predation and collection, or a combination of both factors. However, prior study on taphonomic biases potentially at work in this cave accumulation find no evidence for a change in predators, a change in collection radius, or an adjustment in collection vectors .
The periods of higher effective moisture may provide deeper, more permanent ponds that would offer greater protection from aerial predators, but we cannot conclude that such a sampling bias is the only factor affecting fossil abundance. The significant difference in BSI between paedomorphic and terrestrial adults during interval C, along with the increase in abundance, is evidence for warm climatic conditions that allows indeterminate growth of a terrestrial ectotherm to continue. This agrees with expected changes in A. tigrinum populations during the warming based on modern studies [41, 42].
Our results also demonstrate that a population of A. tigrinum can show changes in average body size without changes in the percentage of different morphologies. In the oldest intervals, E and D, paedomorphs appear to have been smaller (based on BSI) than in other intervals, while the percent paedomorphic remained relatively constant. This suggests a favorable terrestrial environment where metamorphosing individuals continue to grow following the transition from the aquatic environment. This terrestrial growth will be most facilitated by warm conditions and abundant food. The available climate records show these relatively long time periods as variable, with periods of high and low effective moisture [43–45].
There are a variety of confounding factors that complicate our interpretations. First, although the excavation was remarkably rich and the number of identified salamander specimens quite large, our conservative use of MNI reduced our sample size. Our use of time intervals (A-E), while justified based on our own 14C chronology, exacerbated time-averaging . In addition to impacts on the fossils, the intervals create a potential time averaging error for the climatic record. In fact, several intervals span more than one particular climatic period, while certain major climatic periods span more than one interval. Thus, although the fossil record does not indicate significant changes in certain traits, there may be larger variation in those traits that simply is not recorded in the fossil record or that is masked by the effect of time averaging. Also, the overall influence of the abiotic environment may have the most significant effect on Ambystoma metamorphosis, yet we cannot dismiss the role of certain biotic elements. Specifically, density-dependent effects are correlated with body size and metamorphosis, which may skew our interpretations. Larval density will impact resource competition, with higher competition driving metamorphosis. Patterns in larval individuals have not been documented here because of small sample sizes. Finally, likelihood of paedomorphosis may have a significant genetic control, which we do not discount here. Our results support the maintenance of alternate, but coexisting, life history strategies suited to selection pressures in variable environments.
The late Holocene Ambystoma fossils from Lamar Cave provide a unique opportunity to examine the important ecological and evolutionary implications of changes in life history characteristics in response to climatic change. We present a record of change in a population of tiger salamanders over the last 3000 years. Significant perturbation in the environment is possible under future climate change scenarios, and the role of local populations in the terrestrial or aquatic system may be altered. Warmer and drier climate scenarios as predicted for the Yellowstone region  would likely create less permanent aquatic environments and select for populations with primarily metamorphosing individuals, against the retention of paedomorphosis. This scenario would decrease the vertebrate biomass in the aquatic system as well as reduce the predatory pressure on aquatic Ambystoma food sources. Such changes to the ecological system could result in unexpected biological feedbacks. Also, higher percentages and rates of metamorphosis would increase gene flow between populations. This, combined with probable decreases in sizes of populations, has the potential to alter the overall genetic diversity in the meta-population over time, perhaps reducing the ability of the species to respond to further perturbation of the system.
Study site and fossil collection
The Greater Yellowstone Ecosystem (GYE) is often considered one of the last intact, temperate ecosystems in the world. This ecosystem contains all native mammals and few exotics, and is thought to be functioning in a relatively natural state . The GYE is located in northwestern Wyoming, and contains portions of southern Montana and eastern Idaho (center of park: 44° 36' 53.25"N Latitude, 110° 30' 03.93" W Longitude). The core of the GYE is Yellowstone National Park (YNP), which was established as the world's first national park in 1872. The preservation of this park means that we are able to extend current ecological conditions to the recent past.
The A. tigrinum fossils used in this analysis were excavated from Lamar Cave, a paleontological site in YNP. The details of the excavation and stratigraphy are described elsewhere . Isotopic analysis has shown the sampling radius of the cave to be within 8 km (with 95% confidence) . Within this radius there are at least 19 fishless, modern ponds of generally similar permanence that are potential habitat for A. tigrinum. The A. tigrinum samples are most likely from predation in these ponds and surrounding lands. The current study analyzes fossils obtained from 15 of the 16 stratigraphic levels from the excavation (level 11 did not contain any Ambystoma specimens). For the analyses the levels were pooled into five intervals, labeled A-E (youngest to oldest). This aggregation was based on 95% confidence limits around the radiocarbon dating of the intervals .
Abundance was determined by a standardized minimum number of individuals (MNI) . The MNI was taken as the larger of the two values for sacral or cervical vertebrae (axis), since the Ambystoma skeleton contains only one of each of these elements. The abundance levels were then standardized by dividing by the MNI of the wood rat, Neotoma cinerea. Unlike other common small mammals found at this site, wood rats show a constant relative abundance . This pattern is consistent with a broad habitat preference for this species, and is especially important because the wood rat is the main collection agent of the Lamar Cave fossils. Their relative evenness thus indicates taphonomic constancy of the cave , which is corroborated with isotopic analyses .
Because plasticity in growth rate cannot be directly measured in the fossil record, it is inferred from body size in different age classes. The centrum length and anterior width of each specimen were measured with electronic calipers. A body size index (BSI) was created for each specimen by dividing the centrum length by the anterior centrum width .
Percent paedomorphosis by time interval was determined by dividing the standardized MNI of paedomorphic vertebrae by the standardized MNI of all adult vertebrae, defined as Old Larval, Young terrestrial, and Old Terrestrial morphs. Thus, we calculate abundance, mean body size, and percent paedomorphosis, each as potentially independent responses of the salamander population to the abiotic environment around Lamar Cave.
We would like to thank David Ackerly, Yvonne Chan, Kim O'Keefe, Terry Root, Paula Spaeth, Marcel van Tuinen, and S. Randal Voss for comments on earlier versions of this manuscript. We would also like to thank Christian Anderson for assistance with statistical analyses. Thank you to Yellowstone National Park for early funding of this project and access to the study site.
- Gitay H, Suarez A, Diokken JD, Watson RT: Climate Change and Biodiversity. Technical Paper of the Intergovernmental Panel on Climate Change. Edited by: Watson RT. 2002, , IPCC, IV:Google Scholar
- Root TL: Effects of global climate change in North American birds and their communities. Biotic Interaction and Global Change. Edited by: Kareiva PMKJGHRB. 1993, Sunderland, Sinaur Associates Inc., 280-292.Google Scholar
- Barnosky AD, Hadly EA, Bell CJ: Mammalian response to global warming on varied temporal scales. Journal of Mammalogy. 2003, 84: 354-368. 10.1644/1545-1542(2003)084<0354:MRTGWO>2.0.CO;2.View ArticleGoogle Scholar
- Walther GR, Post E, Convey P, Menzel A, Parmesan C, Beebee TJC, Fromentin JM, Hoegh-Guldberg O, Bairlein F: Ecological responses to recent climate change. Nature. 2002, 416: 389-395. 10.1038/416389a.View ArticlePubMedGoogle Scholar
- Root TL, Price JT, Hall KR, Schneider SH, Rosenzweig C, Pounds JA: Fingerprints of global warming on wild animals and plants. Nature. 2003, 421: 57-60. 10.1038/nature01333.View ArticlePubMedGoogle Scholar
- Hoffman AA, Blows MW: Evolutionary genetics and climatic change: will animals adapt to global warming. Biotic Interactions and Global Change. Edited by: Kareiva PMK, Joel G, Huey RB. 1993, Sunderland, Sinauer Associates Inc., 168-178.Google Scholar
- McCarty JP: Ecological consequences of recent climate change. Conserv Biol. 2001, 15: 320-331. 10.1046/j.1523-1739.2001.015002320.x.View ArticleGoogle Scholar
- Parmesan C, Yohe G: A globally coherent fingerprint of climate change impacts across natural systems. Nature. 2003, 421: 37-42. 10.1038/nature01286.View ArticlePubMedGoogle Scholar
- Hobbie JE, Carpenter SR, Grimm NB, Gosz JR, Seastedt TR: The US Long Term Ecological Research Program. Bioscience. 2003, 53: 21-32.View ArticleGoogle Scholar
- Blaustein AR, Belden LK, Olson DH, Green DM, Root TL, Kiesecker JM: Amphibian breeding and climate change. Conserv Biol. 2001, 15: 1804-1809. 10.1046/j.1523-1739.2001.00307.x.View ArticleGoogle Scholar
- Blaustein AR, Wake DB, Sousa WP: Amphibian Declines - Judging Stability, Persistence, and Susceptibility of Populations to Local and Global Extinctions. Conserv Biol. 1994, 8: 60-71. 10.1046/j.1523-1739.1994.08010060.x.View ArticleGoogle Scholar
- Carey C, Cohen N, Rollins-Smith L: Amphibian declines: an immunological perspective. Dev Comp Immunol. 1999, 23: 459-472. 10.1016/S0145-305X(99)00028-2.View ArticlePubMedGoogle Scholar
- Stebbins RC, Cohen NW: A Natural History of Amphibians. 1995, Princeton, NJ, Princeton University PressGoogle Scholar
- Wake DB: Declining amphibian populations. Science. 1991, 253: 860-860.View ArticlePubMedGoogle Scholar
- Petranka JW: Salamanders of the United States and Canada. 1998, Washington, Smithsonian Institution PressGoogle Scholar
- Alford RA, Richards SJ: Global amphibian declines: A problem in applied ecology. Annu Rev Ecol Syst. 1999, 30: 133-165. 10.1146/annurev.ecolsys.30.1.133.View ArticleGoogle Scholar
- Blaustein AR, Wake DB: Declining amphibian populations - a global phenomenon. Trends Ecol Evol. 1990, 5: 203-204. 10.1016/0169-5347(90)90129-2.View ArticleGoogle Scholar
- Carey C, Alexander MA: Climate change and amphibian declines: is there a link?. Divers Distrib. 2003, 9: 111-121. 10.1046/j.1472-4642.2003.00011.x.View ArticleGoogle Scholar
- Donnelly MA: Potential effects of climate change on two neotropical amphibian assemblages. Clim Change. 1998, 39: 541-561. 10.1023/A:1005315821841.View ArticleGoogle Scholar
- Hadly EA: Influence of late-Holocene climate on northern Rocky Mountain mammals. Quat Res. 1996, 46: 298-310. 10.1006/qres.1996.0068.View ArticleGoogle Scholar
- Hadly EA: Fidelity of terrestrial vertebrate fossils to a modern ecosystem. Paleogeogr Paleoclimatol Paleoecol. 1999, 149: 389-409. 10.1016/S0031-0182(98)00214-4.View ArticleGoogle Scholar
- Hadly EA: Evolutionary and ecological response of pocket gophers (Thomomys talpoides) to late-Holocene climatic change. Biol J Linnean Soc. 1997, 60: 277-296. 10.1006/bijl.1996.0094.View ArticleGoogle Scholar
- Hadly EA, Kohn MH, Leonard JA, Wayne RK: A genetic record of population isolation in pocket gophers during Holocene climatic change. Proc Natl Acad Sci U S A. 1998, 95: 6893-6896. 10.1073/pnas.95.12.6893.PubMed CentralView ArticlePubMedGoogle Scholar
- Duellman WE, Trueb L: Biology of Amphibians. 1986, Baltimore, The John's Hopkins Press, 670-Google Scholar
- Voss SR: Effect of temperature on body-size, developmental stage, and timing of hatching in Ambystoma-maculatum. J Herpetol. 1993, 27: 329-333.View ArticleGoogle Scholar
- Camp CD, Marshall JL: The role of thermal environment in determining the life history of a terrestrial salamander. Can J Zool-Rev Can Zool. 2000, 78: 1702-+. 10.1139/cjz-78-10-1702.View ArticleGoogle Scholar
- Bizer JR: Growth-rates and size at metamorphosis of high elevation populations of Ambystoma tigrinum. Oecologia. 1978, 34: 175-184. 10.1007/BF00345165.View ArticleGoogle Scholar
- Semlitsch RD: Paedomorphosis in Ambystoma-talpoideum - effects of density, food, and pond drying. Ecology. 1987, 68: 994-1002.View ArticleGoogle Scholar
- Routman E: Population-structure and genetic diversity of metamorphic and paedomorphic populations of the tiger salamander, Ambystoma- tigrinum. J Evol Biol. 1993, 6: 329-357. 10.1046/j.1420-9101.1993.6030329.x.View ArticleGoogle Scholar
- Wilbur HM, Collins JP: Ecological aspects of amphibian metamorphosis. Science. 1973, 182: 1305-1314.View ArticlePubMedGoogle Scholar
- Sexton OJ, Bizer JR: Life-history patterns of Ambystoma-tigrinum in montane Colorado. Am Midl Nat. 1978, 99: 101-118.View ArticleGoogle Scholar
- Tihen JA: Comments on the osteology and phylogeny of Ambystomatid salamanders. Bulletin of the Florida State Museum. 1958, 3: 1-50.Google Scholar
- Rogers KL: Facultative metamorphosis in a series of high altitude fossil populations of Ambystoma tigrinum (Irvingtonian: Alamosa County, Colorado). Copeia. 1985, 926-932.Google Scholar
- Sprules WG: Adaptive significance of paedogenesis in North-American species of Ambystoma (Amphibia-Caudata) - hypothesis. Can J Zool-Rev Can Zool. 1974, 52: 393-400.View ArticleGoogle Scholar
- Snyder RC: Comparative features of the life histories of Amystoma gracile (Baird) from populations at low and high altitudes. Copeia. 1956, 1: 41-50.View ArticleGoogle Scholar
- Jackson ME, Semlitsch RD: Paedomorphosis in the salamander Ambystoma talpoideum - effects of a fish predator. Ecology. 1993, 74: 342-350.View ArticleGoogle Scholar
- Scott DE: Timing of reproduction of paedomorphic and metamorphic Ambystoma talpoideum. American Midland Naturalist. 1993, 129: 397-402.View ArticleGoogle Scholar
- Semlitsch RD, Wilbur HM: Artificial Selection for Pedomorphosis in the Salamander Ambystoma-Talpoideum. Evolution. 1989, 43: 105-112.View ArticleGoogle Scholar
- Soon W, Baliunas S: Proxy climatic and environmental changes of the past 1000 years. Climate Research. 2003, 23: 89-110.View ArticleGoogle Scholar
- Cook ER, Woodhouse CA, Eakin CM, Meko DM, Stahle DW: Long-term aridity changes in the western United States. Science. 2004, 306: 1015-1018. 10.1126/science.1102586.View ArticlePubMedGoogle Scholar
- Schäuble CS: Variation in body size and sexual dimorphism acrosss geographical and environmental space in the frogs Limnodynastes tasmaniensis and L. peronii. Biological Journal of the Linnean Society. 2004, 82: 39-56. 10.1111/j.1095-8312.2004.00315.x.View ArticleGoogle Scholar
- Mousseau TA: Ectotherms follow the converse to Bergmann's rule. Evolution. 1997, 51: 630-632.View ArticleGoogle Scholar
- Meyer GA, Wells SG, Jull AJT: Fire and Alluvial Chronology in Yellowstone-National-Park - Climatic and Intrinsic Controls on Holocene Geomorphic Processes. Geol Soc Am Bull. 1995, 107: 1211-1230. 10.1130/0016-7606(1995)107<1211:FAACIY>2.3.CO;2.View ArticleGoogle Scholar
- Gennett JA: Palynology and paleoecology of sediments from Blacktail Pond, Northern Yellowstone Park, Wyoming. Geology. 1977, University of IowaGoogle Scholar
- Whitlock C, Bartlein PJ: Spatial variations of Holocene climatic change in the Yellowstone region. Quaternary Research. 1993, 39: 231-238. 10.1006/qres.1993.1026.View ArticleGoogle Scholar
- EPA OP: Climate Change and Wyoming. 1998, , US Environmental Protection AgencyGoogle Scholar
- Patten DT: Defining th Greater Yellowstone Ecosystem. The Greater Yellowstone Ecosystem. Edited by: Keiter RB and Boyce MS. 1991, New Haven, Yale University Press, 19-26.Google Scholar
- Porder S, Paytan A, Hadly EA: Mapping the origin of faunal assemblages using Strontium isotopes. Paleobiology. 2003, 29: 197-204.View ArticleGoogle Scholar
- Grayson DK: On the methodology of faunal analysis. American Antiquity. 1973, 39: 432-439.View ArticleGoogle Scholar
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