Organochloride pesticides in California sea lions revisited

Background Dichlorodiphenyltrichloroethane (DDT) and polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants that have been banned in most countries, but considerable amounts continue to cycle the ecosphere. Top trophic level predators, like sea birds and marine mammals, bioaccumulate these lipophilic compounds, reflecting their presence in the environment. Results We measured concentrations of tDDT (p,p' - DDT + p,p' - DDD + p,p' - DDE) and PCBs in the blubber of dead California sea lions stranded along the California coast. tDDT and PCB concentrations were 150 ± 257 ug/g lipid weight (mean ± SD) and 44 ± 78 ug/g lipid weight, respectively. There were no differences in tDDT or PCB concentrations between animal categories varying in sex or age. There was a trend towards a decrease in tDDT and PCB concentrations from northern to southern California. The lipid content of the blubber was negatively correlated with levels of tDDT and PCBs. tDDT concentrations were approximately 3 times higher than PCB concentrations. Conclusions tDDT levels in the blubber of California sea lions decreased by over one order of magnitude from 1970 to 2000. PCB level changes over time were unclear owing to a paucity of data and analytical differences over the years. Current levels of these pollutants in California sea lions are among the highest among marine mammals and exceed those reported to cause immunotoxicity or endocrine disruption.


Background
The California sea lion, Zalophus californianus californianus, is a useful sentinel for monitoring levels of fat-soluble contaminants in the coastal waters of the western United States. As long-lived, apex predators at the top of a complex ocean food web, with high body lipid content, they become sinks for organochlorines such as PCBs and DDT that, because of their high lipophilicity and persistency, have accumulated in the marine food chain for several decades [1][2][3][4].
Outside Mexico and the Galapagos Islands, the main breeding rookeries of California sea lions are in the Channel Islands in southern California. Pupping occurs from mid-May through June, and breeding follows from July through mid-August. Females remain close to the natal rookery for 6 to 11 months, alternating nursing their pups on the rookery for 1-2 days with foraging trips at sea lasting 2-3 days. In late summer, after breeding, adult and subadult males migrate north along the coast, returning to the rookeries the following summer [5,6]. The distance of migration increases with age, with some adult males migrating as far as British Columbia, Canada. Some non lactating females also leave the rookeries and move up to Central California [7].
The major contaminant pathways in marine mammals are via prenatal mother-offspring transfer and food consumption, including mother's milk. Much of a mother's contaminant load passes directly to her offspring, during nursing, especially to the first one [8][9][10][11]. Males, on the other hand, become increasingly contaminated as they grow older [12][13][14]. After weaning, most contaminants are obtained from consumption of contaminated prey. Organochlorine contaminants cause adverse effects such as immunotoxicity, carcinogenicity, growth and development abnormalities as well as reproductive impairment [4,15]. Mass mortalities and declining stocks among several marine mammal populations from highly polluted areas have been attributed, in part, to contamination by organochlorine pollutants [12,[16][17][18].
Extraordinarily high levels of DDT were reported in California sea lions in the early 1970s [19]. From 1948 to 1970, the world's largest DDT manufacturer, discharged up to 20 tons of DDT wastes annually into the Los Angeles outfall on the Palos Verdes continental shelf in southern California; an estimated 156 tons of DDT residues remain, representing a potential, enduring source of contamination [20]. Since the manufacture of DDT and PCBs stopped in United States in the 1970s, few data have been collected subsequently on contaminant loads in California sea lions [but see [21,22]]. In the present study, we address this omission by evaluating current levels of tDDT and PCBs in the blubber of stranded animals. We focus on geographical and temporal trends and the potential effects of sex and age categories and carcass condition on contaminant levels.

Results
We measured concentrations of tDDT (p,p' -DDT + p,p' -DDD + p,p' -DDE) and PCBs in blubber tissue samples collected from 36 dead California sea lions stranded on islands and the coast of California between April and November, 2000 (Table 1).

Lipid content
The mean lipid content in blubber was low relative to the value of 84-85% reported in apparently healthy sea lions [23]. The condition of the carcass might be responsible for these differences in blubber lipid content among individuals but no significant difference was reported in the lipid content of the blubber between fresh, moderately decomposed and advanced decomposed carcasses. Indeed, some of the extremely low lipid contents were from fresh carcasses. The low lipid content of several animals may have resulted from the mobilization of fat reserves from the blubber prior to death due to starvation or disease, as suggested by Kajiwara et al. [22].
There was a strong negative relationship between lipid content of the blubber and tDDT and PCB concentration in the blubber. This relationship was observed in a previous study on California sea lions [22], which reported that the highest concentrations of both tDDT and PCBs were found in individuals with the lowest blubber lipid content. Similarly, other studies of pinnipeds and cetacea report a negative relation between blubber thickness and concentrations of organochlorines [14,[24][25][26]. This negative relationship can be explained by the fact that fat-soluble contaminants such as tDDT and PCBs are less easily mobilized from the blubber than lipids during fasting and thus tend to accumulate in the remaining blubber layer [24,27].

Sex and age categories
The usual pattern observed in marine mammals is an increase of fat-soluble contaminants such as PCBs and tDDT in the tissues of males and immature females, while in mature females, levels decrease or remain unchanged, as they excrete PCBs and tDDT through the fetus and nursing. As a consequence, males are generally more contaminated than females and juveniles [9,10,[12][13][14]. This has not been documented specifically in California sea lions and their habits make it difficult to do so. Males migrate north of the area for half the year while lactating females stay in the Southern California Bight all year round in close proximity to the tDDT hotspot near Palos Verdes. Sample sizes in this study were insufficient to adequately test for sex and age differences. Moreover, the females collected in central California may have been non-lactating, not yet parous, or simply younger, having experienced fewer reproductive cycles. More data are needed to address changes in contamination levels as a function of sex or age.

Location
The pattern of contaminant levels as a function of the stranding location observed in the present study was somewhat unexpected. It may, however, be explained in part by the fact that individuals, males in particular, move long distances and feed on a variety of prey, that themselves move significantly. The principal prey of California sea lions are northern anchovy (Engraulis mordax), Pacific whiting (Merluccius productus), mackerel (Trachurus symmetricus), rockfish (Sebastes spp.), and market squid (Loligo opalescens) [28][29][30], highly mobile, vertically and horizontally migrating, schooling species that are widely dispersed on or near the coastal continental shelf with distributions that vary seasonally and annually [31,32]. As a consequence, the concentrations reported here may not necessarily reflect the contamination levels of prey in the area where the sea lions stranded.
tDDT/PCB and DDT/DDE ratios tDDT concentrations in California sea lions were approximately 3 times higher than PCB concentrations, a pattern characteristics of marine mammals from the California coast. This is in contrast to the North Atlantic, Arctic, Gulf of St Lawrence, Mediterranean sea, and the coast of Wash-ington, where PCB concentrations in the blubber of marine mammals are usually as high or higher (up to 1 order of magnitude) than tDDT concentrations [3,9,10,13,[33][34][35][36][37][38][39][40][41]. Only a few studies, such as those on the contamination of Baikal seal (Pusa sibirica), northern fur seal (Callorhinus ursinus) and killer whales (Orcinus orca) in Oregon, report higher levels of tDDT than PCBs [3,12,42]. The scant information on marine mammals from the tropical and equatorial fringe of the Northern Hemisphere generally also report tDDT as the main pollutant, followed closely or equaled by PCBs [43]. Kajiwara et al. [22] observed higher levels of tDDT compared to PCB in the blubber and liver of California sea lions while they found the reverse in the livers of harbor seals (Phoca vitulina) in California. They concluded that California sea lions, stranded in central and northern California probably spent parts of the year feeding in the Southern California Bight while harbor seals, also stranded in central and northern California, remained at the same location throughout the year and thus received exposure only locally [22]. Sea otter (Enhydra lutris) tissues from California also showed higher levels of tDDT compared to PCBs, while in the tissues of the same species from Alaska, the reverse was reported [44,45]. Thus, the tDDT/PCB ratio appears to be higher in California and may originate, at least partly, from the DDT hotspot in the Southern California Bight, the legacy of DDT production and byproduct dumping over 30 years ago.
The high proportion of DDE, a DDT metabolite, in tDDT is in the upper range of reports in the literature for other marine mammal species [22,37,39,40,43,[45][46][47]. A high proportion of DDE reflects the absence of recent DDT contamination. The ratio DDT/DDE in 2000 (0.0032) however is lower than in the studies of Le Boeuf and Bonnell [19] as well as Lieberg-Clark et al. [21] that reported ratios of 0.023 and 0.032, respectively. This suggests that the composition of tDDT contamination is still changing towards a lower amount of DDT. As the proportion of DDE seems to increase with age in adult males [10], differences between studies may vary in part with the number of older males in each sample.

Temporal trends
To better assess the decline in tDDT levels over the last 30 years and to avoid the potential confounding factor of sex, we compared males in this study with blubber lipid contents higher than 20% with a similar sample of males reported in previous studies [19,21] (Fig. 3). Blubber tDDT levels were more than 90% lower in 2000 than in 1970. Even so, an accurate estimation of the rate of decrease is elusive because analytical methods for organochlorines have improved in accuracy over the last 30 years [43,47]. Packed columns, commonly used before the 1980s, underestimated DDT concentrations by 30-40% [43]. In contrast, Addison and Stobo [47] mention DDT-group analyses that were internally consistent from the early 1970s to the early 1990s. In addition, a potential overestimation of tDDT levels due to their co-elution with some PCB congeners was noted for the California sea lion samples in the 1970s [25]. Nevertheless, the main interference would have been the co-elution of p,p'DDT with PCBs, which represents a small proportion of tDDT [ [48] in [21]]. Thus, we conclude that the decline of tDDT during the last 30 years was over one order of magnitude.
The decrease in tDDT in California sea lions during the last 30 years, although impressive in magnitude, is in general accordance with declines in other marine mammals [43]. Moreover, although the mean level of tDDT is much lower in 2000 than in 1970, inter-individual variation remains high; several individuals in 2000 exhibited concentrations within the range of concentrations reported in 1970.
It is difficult to draw conclusions concerning the trends of PCBs during the last few decades. The studies in the 1970s reporting PCB concentrations in California sea lions dealt only with lactating females [23,49]. Moreover, the techniques for analyzing PCBs in the 1970s (packed columns) overestimated the levels by about 50% [43]. PCB concentrations were also expressed as Aroclor mixture and not in a congener-specific approach [43,47]. In general, a decrease in PCB levels in marine mammal tissues is often reported [43]. The magnitude of the drop seems to vary with time as well as with geographical areas. No systematic trends, or even increases in levels, are observed in some regions such as the Antarctic [43].

Inter-species differences and geographical trends
Despite the marked decline in tDDT in California sea lions over the last 3 decades, levels remain higher in this animal than in most other marine mammals, even when only samples with blubber lipid content >20% were taken into account. Mean tDDT concentrations in the blubber of California sea lions from the present study are higher (up to 2 orders of magnitude) than current levels reported in other marine mammal species, such as harbor seals, harp seals (Phoca groenlandica), grey seals (Halichoerus grypus), white whales (Delphinapterus leuca), fin whales (Balaenoptera physalus), bottlenose dolphins (Tursiops truncatus), harbor porpoises (Phocoena phocoena) from other geographical areas [27,[38][39][40]43,47,50]. Similarly, PCB levels also appear to be higher than in several other species and geographical areas, although the difference is more moderate. In general, marine mammals from the West Coast of North America display among the highest DDT and PCB loads observed [43].
California sea lions are among the most highly contaminated species along with northeastern Pacific killer whales, St. Lawrence beluga whales, Mediterranean striped dolphin (stenella coeruleoalba), and Mediterranean bottlenose dolphins [42,43,51,52]. It is remarkable that California sea lions exhibit PCB levels that are of the same order of magnitude as those reported in polar bears (Ursus maritimus) from Svalbard, which feed at a higher trophic level, while the tDDT concentrations in California sea lions are 2 to 3 orders of magnitude higher than those of polar bears [36]. Several factors such as sex, age, feeding habits, physiological status (sick, lactating, starving) and analytical techniques exert an influence on organochlorine concentrations and might be partially responsible for the difference between species and geographical areas.

Toxicological effects
Despite relatively high concentrations of tDDT and PCBs, there is no evidence that population growth or the health of individual California sea lions have been compromised. The population has increased throughout the century, including the period when DDT was being manufactured, used, and its wastes discharged off southern California. Few attempts to measure toxicological endpoints in California sea lions have been made [23,49]. The increase in their numbers suggests a healthy population but does not necessarily mean that the contamination by these organochlorines has not exerted an effect on individuals. The concentrations of PCBs reported in the present study are above the 17 mg/kg lipid weight threshold that elicits effects at physiological levels on endocrine and immune systems [53,54]. DDT and its metabolites have been shown to be hormone-disrupters, and to induce estrogenic as well as anti-androgenic effects on several species [55][56][57]. Premature pupping in the early seventies was attributed to the contamination by tDDT [23,49], however, the role of DDT and its metabolites in this reproductive failure was not ascertained and remains unclear [25,58]. A real cause-effect relationship between contaminant concentrations and factors of the immune or endocrine system is difficult to determine due to confounding factors. Nevertheless, concentrations reported here are sufficiently high to encourage continued investigation.

Conclusions
tDDT concentrations have decreased significantly in blubber tissues of California sea lions during the last three decades. Systematic changes in PCB levels are difficult to discern, due to a paucity of data and analytical differences over the years.
We found an unexpected trend towards an increase in the contamination levels from southern to northern California. This does not necessarily mean that the northern part of California is more polluted than the southern part, as California sea lions move about widely and feed on mobile prey. The high ratio of tDDT to PCB concentrations in California sea lions is in part derived from the geographical area they inhabit, where higher tDDT concentrations than PCBs are reported, as compared to other parts of the world. No sex or age difference in contamination levels were found but further study and a larger sample size is needed to confirm the lack of a difference.
Despite the noticeable drop of DDT in California sea lion blubber, concentrations remain high compared to other marine mammals around the world. PCB concentrations are as high or higher than those known to induce effects at a physiological level. This is a cause for concern for the animals as well as for humans that feed on contaminated fishes in the area.

Sample collection
Blubber tissue samples were collected from 36 dead California sea lions stranded on islands and the coast of California between April and November 2000 ( Table 1). The location, sex, standard length, estimated age category, and condition of the stranded pinnipeds were recorded. The condition of carcasses was characterized as fresh, moderately decomposed or less than two weeks dead, and in advanced decomposition or estimated to be more than two weeks dead. Animals from southern California were found on the Channel Islands, those from central California at Año Nuevo Island, and those from northern California at Marin, Mendocino and Humboldt counties. A tissue sample weighing approximately 150 grams was excised from the medial ventrum region at the level of the axilla, from outer skin to muscle. Samples were deposited in clean jars and refrigerated until organochlorine analysis was conducted.

Chemical analyses
The tDDT and PCB analyses were conducted as described elsewhere [22]. The PCB standard used for quantification was an equivalent mixture of Kanechlor preparations (KC-300, KC-400, KC-500, KC-600). Concentrations of total PCBs were based on the sum 84 peaks, representing 117 major congeners. These congeners represent all those that could be found in technical PCB preparations and in the environment.

Data analysis
All statistical analyses were conducted using the GLM procedure (SYSTAT 10.0 windows version). In order to normalize the data, we took the square roots of tDDT and PCB concentrations in blubber lipids. Two outliers (individuals C110400-06 and C110400-07, Table 1) were removed from the data analysis because of their extremely high PCB and tDDT levels ( Table 1) which were far outside the range of the whole data set.
The effects of carcass condition (fresh, moderately decomposed, advanced decomposed) on blubber lipid content as well as on the PCB or tDDT levels in blubber lipids were first examined using a one-way fixed-model analysis of variance (ANOVA) since decomposition of the carcass and a resulting change in blubber lipid content can affect PCB or DDT concentration. However, carcass condition had no significant effect on either blubber lipid content or PCB/tDDT concentrations in blubber lipids (see Results section). For this reason and because of the relatively imprecise character of this factor, it was removed from further analyses.
The effect of blubber lipid content of the blubber (lipid), sex and age category (sex-age category), and the location where the sample was collected (location) on tDDT or PCB concentrations in blubber lipids were examined using a three-way fixed-model analysis of covariance (AN-COVA), with lipid as a continuous variable and sex-age category and location as categorical variables. In a preliminary analysis, we tested the homogeneity of slopes. As the interactions with the continuous variable (lipid) were non-significant in both PCB and tDDT analyses (p > 0.05), we assumed parallelism and pooled these interactions with the model mean square error. The effects of lipid, sex-age category and location were then re-tested under the reduced linear model (see Results section).

Authors' contribution
BJL conceived the study, directed collection of samples, conducted data analyses and wrote the manuscript. JG and KK provided comments on manuscript drafts. JG and ST funded chemical analyses and conducted some analyses. NK and KK conducted most of the chemical analyses. CD provided comments on the final draft from the perspective of ecotoxicology, performed statistical analyses, and helped draft the final manuscript.