±Genetic structure of the oak wilt vector beetle Platypus quercivorus: inferences toward the process of damaged area expansion

Background The ambrosia beetle, Platypus quercivorus, is the vector of oak wilt, one of the most serious forest diseases in Japan. Population genetics approaches have made great progress toward studying the population dynamics of pests, especially for estimating dispersal. Knowledge of the genetic structuring of the beetle populations should reveal their population history. Using five highly polymorphic microsatellite loci, 605 individuals from 14 sampling sites were assessed to infer the ongoing gene flow among populations as well as the processes of expansion of damaged areas. Results Population differentiation (FST = 0.047, G'ST = 0.167) was moderate and two major clusters were detected by several methods, dividing the samples into north-eastern and south-western populations, a similar genetic divergence was reported in host oak trees. Within the north-eastern populations, the subgroups mostly corresponded to differences in the collection period. The genetic characteristics of the population might have changed after 2 years due to the mixing of individuals between populations with enhanced migration related to population outbreaks. Because isolation by distance was detected for whole populations and also within the north-eastern populations, migration was considered to be limited between neighbouring populations, and most populations were suggested to be in genetic equilibrium of genetic drift and gene flow. Recent bottlenecks were found in some populations with no geographical bias; however, they were all from newly emerged oak wilt forests. The emergence of oak wilt should have induced intense fluctuations in the beetle population size. Conclusions Because the genetic boundaries coincide, we suggest that the geographical structuring of the beetle was formed by co-evolution with the host species. Our findings indicate the oak wilt expansion process.


Background
Understanding the processes that drive the spread of plant diseases by vector beetles is critical for developing plans to protect against such damage. Population genetic approaches have made great progress toward studying the population dynamics of pests, especially for estimating dispersal (e.g., [1,2]). Here we report the population genetic structure of an oak forest pest, Platypus quercivorus (Murayama; Coleoptera: Platypodidae), and infer the ongoing gene flow among populations.
Japan has lost vast oak tree forests, particularly stands of Quercus crispula Blume, due to wilt, at a rate of approximately 2,000 ha/year [3]. This mass mortality of oaks is caused by Raffaelea quercivora Kubono et Shin-Ito [4] and its vector, the ambrosia beetle P. quercivorus [5,6]. Oak wilt is the first example of a disease caused by an ambrosia fungus carried by an ambrosia beetle that kills vigorous trees [7]. Although the disease was first reported in the 1930s and its emergence has been sporadically reported [8,9], no epidemical symptoms were established before the 1980s [10]. As of 2007, the disease had expanded rapidly, and damage had been reported in 23 of the 46 prefectures in Japan (Figure 1) [11].
The range of the damaged area has been expanding, but where the beetles originated remains unknown. Knowledge of the genetic structuring of the beetle populations should reveal their population history. Such information may also contribute to an understanding of why the oak wilt has been so damaging. Kamata et al. [7] suggested that global climate change has pushed the beetle's distribution northward such that it is now attacking Q. crispula, which is a typical species of northern cooltemperate forests in Japan. In addition, oak wilt is less damaging to Quercus serrata Thunb. ex Murray, which is more common in southern areas, than to Q. crispula [12]. Kamata et al. [13] questioned whether fungus, vector and host species are in evolutionary equilibrium to test the invasive species hypothesis. It is possible that Q. crispula is more susceptible to the disease because of a lack of history of co-evolution between the host and the vector species. Kobayashi and Ueda [14] predicted that changes in the management of oak forests are the main cause of the increase in oak wilt because P. quercivorus was widely distributed in Japan before the epidemics. Specifically, they suggested that the beetles use mature trees, whose prevalence has increased in oak forests following the disuse of firewood. Changes in the distribution of the beetles may be traced using population genetics methods, and the cause of the sudden disease expansion may be inferred using molecular ecological studies.
To develop plans for protection against oak wilt, it is necessary to understand the process by which the vector beetles spread the disease. Temporal and spatial patterns of P. quercivorus infestations have suggested that the damage spreads from an epicentre to surrounding trees [7]. The majority of disease dispersal is found within the small migration range of the vector; however, damaged areas more than 10 km apart have suddenly emerged [14]. For example, in 2005, Nagano Prefecture reported that after gradual diffusion from the northern region of the prefecture, damaged Q. serrata was found 100 km south of the original infestation [15]. It is unclear whether the expansion of the disease is accomplished by colonisation of the vector beetles. There is increasing interest in adopting new technology to estimate dispersal because the movement of organisms from one location to another is integral to the study of evolutionary and ecological dynamics. Genetic markers are valuable tools for analysing the dispersal abilities and mechanisms, and provide an alternative to mark-and-recapture methods (e.g., [16]). Areas of ongoing gene flow are often deduced to describe and interpret patterns of genetic structure. Microsatellite markers in particular exhibit high resolution and are suitable for studying genetic structuring and deducing the ongoing dispersal of pests [17,18]. We used microsatellite markers to map the spatial and temporal population structure of P. quercivorus in areas damaged by oak wilt disease. We investigated the dispersal of P. quercivorus among populations to thereby gain an understanding of the expansion mechanisms of the disease.

Sampling
In 2005 and 2007, individual beetles were sampled from 14 sites (Table 1, Figure 1). Adult P. quercivorus individuals emerge from the trunk of the oak tree and disperse during the summer. We collected adult specimens using an aggregation pheromone [19] and a trap with cross barriers and propylene glycol basins (Sankei). In some newly damaged areas, individuals were collected directly from injured wood or weakened trunks due to the difficulty of trapping flying adults. Samples were collected from damaged areas 0-5 years after the first appearance of oak wilt (Table 1). Site 10 (TRL) represented a population from an area exhibiting newly emergent damage with no reported oak wilt in neighbouring sites. Site 11 (TRT) comprised a population in which no wilting trees were found, and was in close proximity to site 10 (TRL). In Sakae (site 8; SKE), both the flying adults and adults emerging from trunks were collected. All samples were preserved in 99.5% ethanol at -20 or 4°C until DNA extraction.

DNA extraction and microsatellite genotyping
In total, 20-154 individuals were examined per site (Table 1). Total DNA was extracted using PrepMan reagent (Applied Biosystems) or the Chelex method [20] with some modifications [21]. Five microsatellite loci were examined: Pq3003, Pq3313, Pq3422, Pq3423 and Pq3469 [22]. One primer of each primer set (typically the forward primer) was end-labelled with NED (Pq3422, Pq3423), FAM (Pq3003, Pq3469) or HEX (Pq3313) fluorescent dye. The polymerase chain reaction (PCR) was performed in 8-μl volumes with one-fifth volume of the 5 × PCR buffer Ampdirect containing Mg 2+ (Shimadzu Biotech) or one-tenth volume of the 10 × PCR buffer supplied with Ex Taq, 160 μM of each dNTP, 0.4 unit of TaKaRa Ex Taq (TaKaRa), 40 ng of fluorescently labelled primer, 40 ng of the corresponding primer (Applied Biosystems) and genomic DNA. Because the primer pairs Pq3469, Pq3423 and Pq3313 are suitable for multiplex PCR, they were added to one tube with 1/3 concentrates of the mixture described above. Amplification was performed as follows: 94°C for 2 min, followed by 30 cycles of 94°C for 30 s, 53°C for 30 s and 72°C for 45 s. For multiplex loading, 0.5 μl of each PCR product and 0.5 μl of GeneScan 400HD [ROX] Standard (Applied Biosystems) were added to 12 μl of deionised formamide. The products were separated using capillary electrophoresis (ABI PRISM 310; Applied Biosystems) and assigned scores using 310 GeneScan software (Applied Biosystems) and by eye.

Data analysis
Micro-Checker v. 2.2.3 [23,24] was used to check microsatellite data for scoring errors and null alleles. Fundamental genetic parameters were calculated over all loci using the programme POPGENE v. 1.31 [25]. Observed (H O ) and expected heterozygosities (H E ) were calculated to quantify the genetic diversity of each population. Allelic richness (A; [26]) was estimated using a fixed sample size of 20. Putative linkage disequilibrium was assessed between all pairs of loci for each population using GENEPOP [27], and the significance of the P value was corrected using the sequential Bonferroni procedure [28]. F statistics were studied with FSTAT v. 2.9.3 [29], estimated F IS and F ST [30] to test for local inbreeding within populations and differentiation between populations. The significance of deviations from Hardy-Weinberg equilibrium within population was tested by randomisation. The significance of the population differentiation was tested assuming Hardy-Weinberg equilibrium within populations by the permutation test. The genetic diversity and genetic components were compared between trap-collected and trunk-collected samples in Sakae using the indices above. The post hoc test was adjusted using the sequential Bonferroni procedure [28]. Since the absolute values of the population differentiation parameter F ST depend on the level of genetic variations in the examined material, standardised values of G' ST [31], which always range from 0 to 1, were also calculated using averaged values of intra-population gene diversity (H S ), total gene diversity (H T ) and the G ST values calculated by SMOGD [32]. BOT-TLENECK v. 1. 2. 02 [33] was used to detect recent bottlenecks under the assumptions of the infinite allele mutation model (IAM), and the Wilcoxon signed rank test was applied. Severe reductions in population size were inferred from comparisons between the expected equilibrium gene diversity (H E ) and the observed numbers of alleles. An analysis of molecular variance (AMOVA) was performed using the programme ARLEQUIN v. 3.01 [34] to test for a geographical structure effect. An AMOVA was also used to partition the genetic variance among groups, among populations within groups and within populations. Groups were defined as individuals collected in the same year. Upon conducting the AMOVA, F ST was used to quantify the degree of population differentiation. The significance of any differentiation was tested using a permutation method with 10,000 replications. Multidimensional Scaling (MDS) based on Nei's genetic distances D A [35] calculated using Populations 1.2.30 [36] was used to explore the relationship between geographical site and genetic differentiation. MDS was performed using the programme SYSTAT v. 9.01 [37]. Multidimensional scaling operates directly on dissimilarities; therefore, no assumptions about statistical distribution were necessary [37]. This avoided the distortions seen in classical scaling methods, which assume a linear relationship between values (e.g., principal co-ordinates analysis PCoA) [38]. The fit of the data in two dimensions was measured by the stress factor. A spatial analysis of molecular variance (SAMOVA, [39]) algorithm was used to define the population configuration. Given the number of groups (K), the population configuration with the highest differentiation among groups (F CT ) was calculated using a simulated annealing procedure by SAMOVA 1.0 [39]. K was set between 2 and 9 with 500 independent simulated annealing processes, and the optimum number of population groups for a set of sample populations was estimated by exploring the behaviour of the index F CT for different values of K. Clustering of the populations was assessed by generating a neighbour-joining (NJ) tree based on the D A genetic distance using Populations with a bootstrapping test.
"Isolation by distance" (IBD) was tested as the correlation between genetic and geographical distance, using all population pairs to estimate the regression of F ST /(1 -F ST ) on a logarithm of distance for populations, as suggested by Rousset [40]. The relationship between genetic differentiation and geographic distance was assessed by the Mantel test [41], with 9,999 randomisations using GenAlEx 6.2 [42]. This test was done for total populations as well as populations collected in same year (2005 and 2007). The reasoning for the latter analysis is discussed in the Results.
To assess the level of population structure and assignment of an individual's origin, an individual-based clustering was performed using the programme STRUCTURE v. 2.3.1 [43]. F statistics and AMOVA tests used the sample location as the unit of comparison, whereas the Bayesian model-based methods of STRUCTURE used the individual as the unit, assigning it to the most likely group (cluster). Each individual genotype was used to estimate the proportion of admixture from several demes in an individual's nuclear genome. Five independent runs for each K were used, with burn-ins of 20,000 replicates and run lengths of 10,000 replicates. An allele frequency correlated model (F model; [44]) and an admixture model were adopted. STRUCTURE v. 2. 3. 1 is also able to make explicit use of sampling location information to provide accurate inferences [45], and the LOCPRIOR model was used. The likelihood of the assignments was evaluated for K varying from 1 to 9, and the reliability of the K clusters was tested for these values and variances among the trials; ΔK was also used to assess the true K number [46]. In the F model, all K clusters are assumed to be diverged from a common ancestral population at the same time, but the model allows the possibility that the clusters may have experienced different degrees of drift since the divergence event to be considered [47]. In this model, the amount of drift for each cluster from a common ancestral population is described as 'F', values of which are analogous to traditional F ST values between clusters and a common ancestral population [44]. Then, F for each cluster can be used as an indicator of genetic drift and/or bottleneck in the long term [44,47]. F was assessed for each K to search for bottlenecks in the long term.

Allele polymorphisms and genotypic linkage
We were able to successfully genotype 605 individuals from 14 sampling sites using all five loci. No null alleles or scoring errors were detected by Micro-Checker. The number of alleles varied from 8 (Pq3422) to 16 (Pq3423), with an average of 12.4 alleles per locus. Nine of 150 tests [between Pq3003 and Pq3313 in 1 (NKH); Pq3313 and Pq3469, and Pq3422 and Pq3423 in 9 (SNN); Pq3313 and Pq3423, Pq3313 and Pq3469, and Pq3423 and Pq3469 in 10 (TRL); Pq3313 and Pq3469, Pq3422 and Pq3469, and Pq3422 and Pq3423 in 11 (TRT); P < 0.05] showed significant linkage disequilibrium after correcting for multiple tests.

Genetic diversity and Hardy-Weinberg equilibrium within populations and detection of bottleneck populations
The mean expected heterozygosity (H E ) for all loci varied from 0.594 to 0.741 (Table 2). For most populations, the expected and observed heterozygosities were similar and F IS values were not significantly different from 0, except for population 1 (NKH; P < 0.001). The mean number of alleles (Ā) within populations ranged from 4.6 to 7.2. Beetles emerging from trunks and beetles collected using the pheromone trap were similar in their genetic diversity at site 8 (SKE), and no genetic differences were detected between them (F ST = -0.0018, P = 0.59429). Thus, we combined the data for site 8 (SKE) for further analyses.
Recent genetic bottlenecks were detected within sites 1 (NKH), 5 (BDU), 6 (TMG), 8 (SKE), 11 (TRT), and 12 (NGY; Table 2). Populations showing significant recent bottlenecks were distributed throughout north-eastern and south-western populations; however, they were all from newly emerged oak wilt forests for which the first damage occurred within the 2 years prior to sampling.

Population differentiations
Moderate population differentiations were found (F ST = 0.047 ± 0.002, G' ST = 0.167 ± 0.001; P < 0.001). Pairwise F ST estimations are shown in Table 3. The AMOVA showed that the variance within populations explained 95.3% of the total variance; 4.7% was expressed among populations and the overall population differentiation was significant (P < 0.001). The hierarchical AMOVA indicated that the effect of collection year was marginally significant and the collection site effect was significant ( Table 4) (Figure 3). From K = 2 to 5, F CT increased sharply and then began to level off. Thus, we used K = 2-5 for interpreting the grouping patterns. The first level of divergence defined two groups, 12 (NGY) and others. When K = 3, population 12 (NGY) and the Tenryu populations, 10 (TRL) and 11 (TRT), are considered to be different groups from the others. In addition, the increase in K substantially increased the F CT . Four clusters were revealed as follows: cluster 1 included 1 (NKH), 6 (TMG), 7 (OMY), 8 (SKE) and 9 (SNN); cluster 2 included 2 (YUZ), 3 (SAK), 4 (TOK) and 5 (BDU); cluster 3 included 10 (TRL) and 11 (TRT); and cluster 4 included 10 (NGY), 13 (ODN) and 14 (MMD). Cluster 1 comprises populations distributed in the north-eastern region that were collected in 2007 with the exception of 9 (SNN). Cluster 2 is composed of the 2005 collections, and the distribution of cluster 2 overlaps that of cluster 1. Clusters 3 and 4 are composed of populations from the south-western regions. When K = 5, cluster 4 is split in two: one contains10 (NGY) and the other contains 13 (ODN) and 14 (MMD). The clustering of K = 4 and K = 5 was supported with high bootstrap values in the NJ tree using Nei's D A (Figure 4). When K = 4, two major groups were detected corresponding to clusters 1 and 2 and clusters 3 and 4, supported with 88% and 93% bootstrapping values for clusters 1 and 2, respectively, and by 92% and 60% for clusters 3  The Mantel test showed that the between-population differentiation increased significantly with geographical distance (r = 0.520, P < 0.001), as illustrated in Fig 5, suggesting a model of IBD. Given that in north-eastern populations, genetic change was detected between the two collection years (2005 and 2007), the IBD was tested separately for each year. Significant IBD was confirmed for each test (2005, r = 0.714, P < 0.001; 2007, r = 0.495, P = 0.001). In addition, the IBD within north-eastern populations in the 2005 collection [2 (YUZ), 3 (SAK), 4 (TOK), 5 (BDU) and 9 (SNN)] was tested to eliminate any effects of differentiation between the north-eastern and south-western populations and the collection year. IBD was also detected in the north-eastern populations for the 2005 collection (r = 0.861, P = 0.046).

Individual-based clustering
The Bayesian clustering analysis conducted using STRUCTURE showed substantial genetic structuring for P. quercivorus. The likelihood of assignment, ln P (D), increased from K = 1 to 7 ( Figure 6). It then fluctuated, and the highest value was obtained at K = 9. The standard deviation of this value for each iteration increased with the value of K, and was very high when K > 5. The highest ΔK was found at K = 2, whereas it decreased to almost 0 when K = 5 ( Figure 6). Therefore, the consistency of the result was unclear from K = 5. Because adding a cluster from K = 5-6 did not provide much more information regarding the genetic structuring (Figure 7), assignment results were interpreted for K = 2-5. Populations were clearly divided into north-eastern and south-western clusters when K = 2, and there appeared to be few migrant individuals. When K = 3, the north-eastern cluster was partitioned and most populations exhibited some admixturing, with the exception of 1 (NKH). This partition corresponded to the subgrouping shown in the SAMOVA and NJ analyses. Populations in Tenryu [10 (TNL), 11 (TNT)] were divided from the south-western cluster when K = 4. Population 12 (NGY) and populations in the Kyoto prefecture [13 (ODN) and 14 (MMD)] were discriminated when K = 5. These partitionings were also consistent with those of the SAMOVA and NJ tree analyses. Higher F values were exhibited in the north-eastern clusters when K = 2-5 ( Figure 7).

Genetic diversity and population bottleneck
Our data indicate that high genetic diversity exists within populations regardless of the location or collection method. When samples from population 8 (SKE) were compared for their genetic diversity and components, the Table 3 Population differentiation estimated F ST (above the diagonal) and significance for each pairwise comparison (below the diagonal)  collection method (trapping flying adults or direct collection from trunks) had little effect on the overall results. Because no null alleles were detected, all of the genotyped data were considered useful for the analysis.
A bottleneck was detected in some populations from recently infested forests. Since there was no damage, these individuals may have originated as a small number of colonisers, which would have entailed a founder effect. If they were not colonisers, they may have been residents, in which case sudden changes in the availability of resources might have resulted in fluctuations in the population size of the beetle in damaged oak forests.
Linkage disequilibrium was also detected in recently infested forest area populations. The lack of linkage disequilibrium in most populations suggests that the loci used may not be in close proximity to one another on the chromosomes. Population bottlenecks and/or admixturing of differentiated populations (mentioned below) might be related to linkage disequilibrium in populations from recently infested areas.

Oak wilt damage expansion and P. quercivorus demographic history
The moderate level of population differentiation (F ST = 0.047, G' ST = 0.167) detected in P. quercivorus suggests that gene flow among populations is balanced with genetic drift for the entire study area. Genetic analysis using microsatellite markers revealed two main genetic   groups of P. quercivorus in Japan. These are divided into north-eastern and south-western populations. Using chloroplast DNA, Okaura et al. [48] examined the phylogeography of Q. crispula and three related species, which are the main hosts of P. quercivorus. Quang et al. [49,50] showed nucleotide functional gene variation in Q. crispula and inferred its history of colonisation into Japan. These studies showed that a genetic boundary for Q. crispula was found between the north-eastern and south-western populations and coincides with the Itoigawa-Shizuoka Tectonic Line, a major tectonic depression that runs through Nagano Prefecture and separates sites 1 (NKH) through 9 (SNN) from 10 (TRL) through 14 (MMD). Given that the beetles exhibited the same pattern of genetic differentiation between the eastern and western populations, it is possible that they share a population history with Quercus species. A change in the vegetation distribution would likely affect the beetle's genetic structuring in evolutionary timescales. Kamata et al. [7] predicted that P. quercivorus expanded its distribution northward and upward in elevation, and that the lack of co-evolution between Q. crispula and the beetle had an influence on the damage that incurred. They further proposed that a recent warmer climate has altered the distribution of P. quercivorus such that they encountered Q. crispula,  Figure 6 Estimation of the true number of clusters by STRUCTURE analysis [43]. Mean likelihood of K and the standard deviations of five runs (left axis) and ΔK [46] (right axis) for K = 1 to 9.  Figure 7 The proportion of the membership coefficient of 605 individuals in 14 populations for each of the inferred clusters for K = 2 to 6 in the STRUCTURE analysis [43]. Individuals are represented by vertical lines, and the different colours show the probabilities of ancestry from the hypothetical genetic populations partitioned into K clusters. The F value for each cluster is also shown to the right.
which is lacking in defence systems against the disease. Given that the genetic structuring of P. quercivorus suggests that the species became established in accordance with the vegetation history on a regional scale, it is difficult to support Kamata et al.'s hypothesis [7] for the main cause of oak wilt expansion in entire regions.
Although the genetic structures of the hosts and vectors coincide, the hypothesis that individuals from different origins invaded Japan and caused population structuring divided by the Itoigawa-Shizuoka Tectonic Line by chance cannot be rejected. Further details of the population history of the beetles may be elucidated by additional phylogenetic analysis using individuals from outside Japan. A rapid expansion of species distribution often entails a lack of geographical structuring [2,51]. The beetle populations that are damaging Q. crispula in north-eastern areas are not considered exclusively invasive populations because IBD was detected within the north-eastern populations. According to Rousset [40], most populations are thought to be in genetic equilibrium between genetic drift and gene flow. However, we identified a single population that may be composed of migrants. Specifically, the population at site 1 (NKH), which represents the northern limit of oak wilt in 2007, exhibited inbreeding, and an analysis using the STRUCTURE programme predicted that the population was a result of admixturing of two groups. Therefore, the damage at site 1 (NKH) may have been caused by non-local individuals from various source populations.
There was no visible damage near site 10 (TRL), and it was not clear whether the beetles therein that attacked Q. serrata were endemic or migrant. Adults were caught by trapping within site 11 (TRT), and there was no oak wilt. Site 11 (TRT) is near to site 10 (TRL). Beetle populations from sites 11 (TRT) and 10 (TRL) showed similar genetic compositions with high genetic diversity. Therefore, they may not represent descendants of long-distance dispersers from severely damaged areas, and may instead be endemic populations. The beetle populations within sites 10 (TRL) and 11 (TRT) must have persisted without a break.

Population expansion and migration of P. quercivorus
The NJ, SAMOVA and clustering by STRUCTURE analyses indicated that within the north-eastern populations, genetic components were substituted between 2005 and 2007. Because IBD was detected among northeastern populations in 2005, most of the migration should be limited to neighbourhoods. This substitution may have been attained by gradual expansion of population distributions and mixing of population structures prior to the emergence of oak wilt damage. The migration of the beetles among populations might be enhanced by increased oak wilt damage. Therefore, samples collected in 2007 in north-eastern regions may contain individuals from broader areas. Although the beetles may seldom disperse long distances beyond a regional scale, migration within a regional scale may be frequent.
Chapuis et al. [18] examined population outbreak effects using the locust Locusta migratoria. They showed that population outbreaks reduce population differentiation by enhancing migration and/or effective population sizes. Thus, an increased P. quercivorus population size might alter the population structure in a similar manner to that seen with L. migratoria. Our STRUCTURE analysis produced an F value that indicated a high value for clustering of individuals in north-eastern populations mostly collected in 2005 when K = 3. However, the F value decreased in the 2007 collection cluster. This result indicates that the north-eastern population collected in 2005 was influenced by strong genetic drift after divergence from the common ancestral population, and that the effective population size is small. By incorporating independent demes, outbreak populations from 2005 to 2007 might have made the effective population sizes larger.

Implications for developing protection plans against oak wilt
The genetic structure of the beetles supported Kobayashi and Ueda's prediction [14] that changes in the management of oak forests are the main cause of the increase in oak wilt. Although migrant beetles might occasionally produce new areas of oak wilt damage, our analysis of the genetic structuring of the vector beetle populations indicated that the invasion of the beetle via long-distance dispersal into non-endemic areas or distribution change above regional scale may not be the main cause of oak wilt expansion. Outbreak populations might have enhanced migration on a small scale, and beetles in neighbouring populations may have encouraged such activity. Population outbreaks may also have led to mass attacks on host trees in neighbouring forests, thus establishing new damaged areas. The sequence of activation of the beetle might have promoted disastrous oak wilt.
To prevent the expansion of the disease to undamaged areas, it is important to manage beetle populations to maintain low densities and thus stopping the sequence of activation. Therefore, we need to trap beetles and/or monitor oak wilt damage to provide an indication of the population density of the beetles in continuous oak forests. Once damage is confirmed, the health of the oak forest can be maintained if immediate action is taken before Allee effects (in which the growth rate of sparse populations increases with increasing population density; [52]) become severe. In particular, in mature oak forests composed of plenty of large-diameter trees, vast numbers of beetles can emerge from a few wilted oaks [53]. We must monitor oak forests that have been abandoned for a long time and are full of large trees with caution, so as not to release pests to nearby forests. Further studies of the dispersal dynamics of the beetles, such as frequency and distance of adult flight, are needed to generate alert systems for oak wilt expansion.

Conclusions
We found genetic differentiation in the ambrosia beetle P. quercivorus, which corresponds to the genetic structuring of the host oak trees. Based on a combination of methods of statistical analysis, the genetic structure is presented with a high degree of confidence. The geographical structuring of the beetle was thought to be formed by co-evolution with its host species.