Fig. 6

Relative white gene expression in consecutive generations after feeding additives. White gene expression was quantified using male heads from flies that were fed with either DMSO (DMSO) or Tranylcypromine dissolved in DMSO (Tranyl) in P/F0//F1 generations. Control flies raised on standard food without Tranylcypromine or DMSO. All flies were from the experiment shown in Fig. 3. Data were generated using n technical replicates from x different biological replicates (br): control br x = 7, n = 43; DMSO F1 x = 1, n = 6; T in DMSO F1 x = 1, n = 7; DMSO F2 x = 3, n = 16; T in DMSO F2 x = 3, n = 12; DMSO F3 x = 3, n = 20; T in DMSO F3 x = 4, n = 30; DMSO F4 x = 3, n = 10; T in DMSO F4 x = 4, n = 12; DMSO F5 x = 3, n = 24; T in DMSO F5 x = 4, n = 23. Student’s T test (one-sided, homoscedastic) to control: DMSO F1, T in DMSO F1, DMSO F2, T in DMSO F2, T in DMSO F4, DMSO F5 all p***, T in DMSO F3 p**. DMSO F5 to T in DMSO F5 p***