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Fig. 3 | BMC Ecology

Fig. 3

From: Transgenerational effect of drug-mediated inhibition of LSD1 on eye pigment expression in Drosophila

Fig. 3

Feeding additives Tranylcypromine or DMSO affect eye colour expression. a Transgenerational effect on eye colour expression after feeding with Tranylcypromine in F0/P//F1 generation. For each culture vessel, Tranylcypromine was dissolved in DMSO (2.5 mg Tranylcypromine in 250 µl DMSO), diluted 1:8 in water and finally mixed with dry yeast. Eye colours of every generation were measured by using 3 × 20 male heads, i.e. a total of 60 male heads per measurement. Four individual experiments with a total of 8 biological replicates for the control and 12 biological replicates for Tranylcypromine feeding comprising a total of n individual measurements (control: n = 85 × 20 male heads, F0/P: n = 12 × 20 heads, F1: n = 6 × 20 heads, F2: n = 16 × 20 heads, F3: n = 42 × 20 heads, F4: n = 36 × 20 heads, F5: n = 39 × 20 heads, F6 n = 39 × 20 heads). Student’s T test (one-sided, homoscedastic) to control: F0/P **, F1 ***, F2 ***, F3 ***, F4 Ø, F5 Ø, F6 Ø. B) Effect of the solvent DMSO on eye colour. 250 µl DMSO was diluted 1:8 in water and the solution used for preparation of the yeast feeding mixture per culture vessel. DMSO treatment exclusively in F0/P//F1 generation. Three individual experiments comprising 8 biological replicates for the control and 5–6 biological replicates for DMSO treatment. Due to a strong effect of DMSO on viability survival of F1 flies is severely impaired. n individual measurements: control n = 92 × 20 male heads, F0/P n = 8 (× 20 male heads), F1 n = 2, F2 n = 9, F3 n = 18, F4 n = 18, F5 n = 17, F6 n = 18, F7 n = 3, F8 n = 9. Student’s T test (one-sided, homoscedastic) to control: F0/P Ø, F1 ***, F2 ***, F3 ***, F4 **, F5 Ø, F6 ***, F7 Ø, F8 Ø

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