Fig. 2

Feeding of Tranylcypromine dissolved in water on eye pigment expression. a Eye colour expression in male flies fed with Tranylcypromine (1.25 mg up to 4 mg dissolved in water per culture vessel) in F0/F1 generation. Flies of consecutive generations were flipped onto fresh culture medium without additive, and after egg-laying prepared for analysis. Eye colour measurement was done in triplicates (using 3 × 20 male heads for each probe). Three independent experiments comprising a total of six biological replicates for the control (untreated flies), and 11 biological replicates for Tranylcypromine treatment (control n = 45 × 20 heads, F0/P n = 9x, F1 n = 26x, F2 n = 18x). Student’s T test (one-sided, homoscedastic) to control: F0/P Ø, F1 **, F2 Ø. Data are normally distributed. b Relative white gene expression in adult male fly heads of consecutive generations after feeding with Tranylcypromine (1.25 mg dissolved in water per culture vessel) in F0/F1 generation. Relative white gene expression is significantly different compared to the controls in the F3 generation (p**; Student’s T test, one-sided, homoscedastic). Expression of white was related to actin expression in each probe and the fold change calculated to the mean relative expression in the control. Control flies (altogether five biological replicates and n technical replicates: F1 (n = 8) + F2 (n = 3) + F3 (n = 8)), F1 flies (one biological replicate and six technical replicates), F2 flies (two biological replicates and six technical replicates), F3 flies (three biological replicates and nine technical replicates)