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Fig. 1 | BMC Ecology

Fig. 1

From: Stagewise resolution of temperature-dependent embryonic and postembryonic development in the cowpea seed beetle Callosobruchus maculatus (F.)

Fig. 1

Embryonic and early larval development in C. maculatus as visualized with (ap) confocal laser scanning microscopy and (qs) light microscopy. Embryos stained with SYTOX Green fluorescent nucleic acid stain: (af) selected cleavages: 2, 16, 64, 256, 1024, and 4096 nuclei, respectively; (g) a mitotic wave during transition from 4096 to 8192 nuclei; (h) invagination of a transverse furrow during gastrulation; (i) a three-layered, segmented germ band; (jl) germ band extension; (m) germ band retraction; (n) the end of germ band retraction and the onset of dorsal closure; (op) dorsal closure; in paired images (ip), the upper and the lower parts correspond to a ventral and a dorsal view, respectively, and belong to different but same-stage embryos, except for (n) that shows the same embryo from both sides. Late embryos at different stages of sclerotization and hatching: (q) reddening of the mandibular tips, ventral view; (r) hatching, viewed ventrolaterally – note that the fully formed larva has rotated 180° and is now emerging through the former dorsal side of the egg; (s) freshly hatched first instar larva, ventral view

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