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Table 2 Sequence of 18 RAPD-PCR primers, total number of scored bands, number of polymorphic bands, percentage polymorphism and polymorphic information content of Neorautanenia brachypus leaf samples

From: Phenotypic and genetic characterisation revealed the existence of several biotypes within the Neorautanenia brachypus (Harms) C.A. wild accessions in South East Lowveld, Zimbabwe

Primer

Primer sequence 5′ 3′

Total number of bands

Number of polymorphic bands

Percentage polymorphism

Polymorphic information content (PIC)

OPE-03

CCAGATGCAC

8

6

75.00

0.1327

OPL-03

CCAGCAGCTT

21

18

85.71

0.1873

OPAS-14

TCGCAGCGTT

6

4

66.67

0.1429

OPAB-17

TCGCATCCAG

11

11

100.00

0.1748

OPN-11

TCGCCGCAAA

8

8

100.00

0.1451

OPAL-14

TCGCTCCGTT

11

11

100.00

0.1497

UBC-434

TCGCTAGTCC

11

10

90.91

0.1439

UBC-737

GGTGGGTGTG

29

26

89.66

0.1534

UBC-738

GGTGGGTGGT

40

34

85.00

0.1747

UBC-742

CCTCCCTCCT

11

10

90.91

0.1327

UBC-743

CCACCCCAC

24

13

54.17

0.1622

UBC-744

CCACCCACCA

13

12

92.31

0.1439

UBC-745

GGGAAGAGGG

14

14

100.00

0.1514

UBC-746

GGGTGTTGGG

44

37

84.09

0.1487

UBC-740

GGAGGGAGGA

28

18

64.29

0.1851

OPAY-10

CAAGGCCCCT

22

14

63.64

0.1681

OPAY-11

ACGCGCCTTC

29

22

75.86

0.1727

OPAY-12

CTGTCGGCGT

21

18

85.71

0.1577

Total

 

368

293

–

 

Average

 

17.52

13.95

77.51

0.157