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Table 5 Number of OTUs common to UPARSE and SWARM output at multiple clustering thresholds

From: A metabarcoding framework for facilitated survey of endolithic phototrophs with tufA

 

UPARSE

99 %

98 %

97 %

96 %

95 %

SWARM

 

1070

918

822

761

698

d01

2483

1044

913

818

752

696

d04

1416

963

902

813

750

693

d08

1208

851

845

806

748

690

d09

1135

835

829

804

747

689

d10

1093

833

827

802

747

689

d11

1066

833

827

802

747

689

d12

1047

833

827

802

747

689

d13

1032

833

827

802

747

689

d14

788

735

735

735

719

676

d15

763

722

722

723

708

673

d16

740

706

706

707

696

668

  1. Recommended global threshold for UPARSE (97 %) and determined local threshold optima for SWARM (d10–d13) are in bold italics. The 802 OTUs kept for subsequent analyses are italicized