Fig. 4

Metabarcoding tufA. a Location of the metabarcode along 1251 bp of tufA displaying sites with conserved A/T (orange) and G/C (blue) nucleotides, insertion-deletions regions (grey), and percentage of maximum entropy (as moving average). Note the lower proportion of conserved sites (i.e. greater informativeness) within the metabarcode. b Site conservation within the forward (top) and reverse (bottom) priming regions. All of the above were produced with (or from data output from) WebLogo 3.4