Table 1 Minimum and maximum values for the image analysis parameters calculated by PHLIP during the development of phototrophic marine biofilms. ANOVA testing determined whether variations in biofilm parameters over time was statistically significant. Significance levels are indicated by asterisks. Channels; concanavaline A (ConA), syto64 (syto), chlorophylla (chla).

Biovolume^a

5.8–28.8

n.s.

0.5–4.7

***

0.1–61.9

***

8.4–82.3

***

Substratum coverage

0.48–0.86

n.s.

0.07–1.63

***

0.03–2.86

***

Area-to-volume ratio

3.5–5.8

n.s.

3.2–5.0

**

1.4–4.4

**

Horizontal spreading^a

8.4–9.1

n.s.

5.8–8.3

**

1.9–7.7

***

Vertical spreading^a

0.09–27.5

***

0.03–24.0

**

0.02–30.4

***

Total spreading^a

8.5–11.7

n.s.

8.3–9.3

*

2.0–10.8

***

Average thickness

15.3–127.4

***

6.3–111.2

*

4.3–102.3

***

6.3–111.2

**

Biofilm roughness

0.15–0.26

n.s.

0.04–0.20

***

0.02–0.30

***

0.24–0.35

n.s.

Co-localization in 2D

5.4–14.5

n.s.

0–3.7

n.s.

0.5–27.9

n.s.

Co-localization in 3D

0.9–5.2

n.s.

0–0.8

n.s.

0.04–2.7

n.s.