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Figure 4 | BMC Ecology

Figure 4

From: Analysis of a marine phototrophic biofilm by confocal laser scanning microscopy using the new image quantification software PHLIP

Figure 4

Development of morphological parameters analyzed from a phototrophic biofilm grown on glass in natural seawater (Oosterschelde, the Netherlands). (A) Temporal development of area-to-volume ratio. Separate channels represent chlorophyll a fluorescence (black bars), EPS stained with Con A (open bars) and bacteria stained with syto64 (dashed bars). Values indicate mean ± S.D, (n = 21). (B) The correlation between biofilm roughness and biovolume. Filled squares: chlorophyll a fluorescence, open circles: ConA-fluorescence, open triangles: syto64, filled triangles, all channel signal.

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