Figure 1From: Analysis of a marine phototrophic biofilm by confocal laser scanning microscopy using the new image quantification software PHLIPMaximum intensity images (A, B, C) and vertical profiles of biofilm coverage analyzed by PHLIP (D, E, F) of a biofilm grown on glass slides in natural seawater (Oosterschelde, the Netherlands). Images and vertical profiles are shown for representative samples measured at days 3 (A, D), day 25 (B, E) and day 44 (C, F). The red channel and open circles represent bacteria stained with syto64, the green channel and black squares represent EPS labeled with ConA and the blue channel and crosses show chlorophyll a autofluorescence. CLSM maximum intensity images represent areas of 250·m × 250·m.Back to article page