Resistance of rumen bacteria murein to bovine gastric lysozyme

BMC Ecology

Table 1 Percentage of growth of control cultures at 6 hours, of Gram+ and Gram- bacteria grown in the presence of EW lysozyme, BG lysozyme and BGE.

ORIGIN	BACTERIUM		Egg White Lysozyme150 ug/ml		Bovine Gastric Lysozyme150 ug/ml		Bovine Gastric Extract10 mg/ml
			pH 5.5	pH 7.0	pH 5.5	pH 7.0	pH 5.5	pH 7.0
ENVIRONMENTAL, INTESTINAL	Gram positive	M. luteus	*lysed* ¹	*lysed* ¹	*lysed* ¹	*lysed* ¹	*lysed* ¹	*lysed* ¹
	Gram negative	P. aeruginosa	106	173	142	182	0	90
		E. coli	103	91	86	124	15	44
RUMEN	Gram positive	L. vitulinus	1	4	81	69	10	8
		L. acidophilus	0	NG	64	NG	168	NG
		S. bovis	101	6	108	114	98	111
	Gram negative	S. ruminantium	109	112	130	96	89	87
		P. ruminicola	NG	24	NG	113	NG	208

¹= cells lysed by treatment in scattering assay. Italic bold numbers = growth inhibited. NG = no growth of culture at this pH.

ISSN: 1472-6785