Figure 3

Cluster analysis (Ward's method, squared Euclidean distance) showing similarities between 35 hybridized microarrays hybridized with probes from wildtype (WT), antisense Lox-3 (AS LOX) and antisense TD (AS TD) of the diploid native tobacco species, Nicotiana attenuata plants and from untransformed plants of 3 congeneric Nicotiana species, two of which are allotetraploids N. quadrivalis and N. clevelandii and that are thought to have N. attenuata as a common ancestor as well as the more distantly related, N. longiflora. Arrays were hybridized with probes from plants attacked by different herbivore species or elicited with methyl jasmonate (MeJA). The shaded box represents 3 replicate hybridizations of the same sample of m-RNA from LOX N. attenuata plants. Arrays included in brackets correspond to clusters in the PCA (Fig. 4).