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Table 1 Number of individual-plant DNA samples harvested for AFLP and ISSR analysis of plants grown from seed collected from: A) six sites within Utah collected over multiple years; B) three burns and 4 roadside washes within Utah collected in 1999; C) 3 non-Utah collections.(Codes identify samples in Fig. 3 and Table 5 [see Additional file 1]

From: ISSR and AFLP analysis of the temporal and spatial population structure of the post-fire annual, Nicotiana attenuata, in SW Utah

Location

Seed collection Year

Codes

SET I Used for only AFLP

SET II Used for ISSR & AFLP

Burn(B) or Wash (W)

A. Time series from the following Utah sites:1

1. Motoqua roadside wash and burn (fire in 1994)

Wash

1990

M1

4

 

W

Lower Burn

1995

M2

3

 

B

Middle burn

1995

M3

3

 

B

Middle burn

1996

M4

8

4

B

Lower Burn

1996

M5

3

3

B

Wash

1999

M6

4

8

W

2. DI Ranch burns (yearly fires at garbage dump)

 

1988

D1

4

 

B

 

1990

D2

1

 

B

 

1992

D3

6

 

B

 

1993

D4

2

 

B

 

1995

D5

4

4

B

3. Goldstrike roadside washes

#2

1990

 

2

 

W

#5

1990

 

1

 

W

#2

1993

G1

5

5

W

#4

1993

G2

2

2

W

#5

1993

G3

1

1

W

4. Shivwits Reservation roadside wash # 1

 

1988

 

1

 

W

 

1990

 

2

 

W

 

1992

 

1

 

W

 

1993

 

4

 

W

 

1995

 

1

 

W

 

1999

B7

8

8

W

5. Shivwits Reservation roadside wash # 2

 

1992

B1

1

 

W

 

1993

B2

3

 

W

 

1995

B3

4

 

W

 

1996

B4

5

 

W

 

1999

B5

9

8

W

 

1999

B6

1

 

W

6. Pahcoon Spring roadside wash

 

1990

 

1

 

W

 

1999

P9

9

8

W

B) Burn and roadside wash populations collected in Utah 1999

1. Pahcoon Spring Burn (fire in 1998)

Burn transect 1

1999

P1

9

6

B

Burn transect 2

1999

P2

7

5

B

Burn transect 3

1999

P3

9

8

B

Burn transect 4

1999

P4

10

8

B

Burn area 1

1999

P5

9

8

B

Burn area 2

1999

P6

9

8

B

Burn area 3

1999

P7

9

7

B

Burn area 4

1999

P8

8

8

B

2. Rt 91 Burn (fire in 1998)

Burn area 1

1999

R1

8

6

B

Burn area 2

1999

R2

8

8

B

3. Single collection roadside washes and burns

Shivwits Reservation 4

1999

B8

9

8

W

Rt-91

1999

R3

9

7

W

Lytle Ranch Preserve

1999

L1

10

10

W

Jackson Spring

1999

J1

10

10

W

Cedar Pockets

1999

C1

10

10

B

C) Non-Utah collections

     

Arizona

1996

 

3

3

 

Oregon

1994

 

2

2

 

California

1999

 

2

2

 

Total plants

  

244

175

Â