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Table 1 Key to grass identification at Sourhope using trn L PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trn L PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

From: Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trn L (UAA) intron

trn L PCR Restriction digest 1 Restriction digest 2 Identity (accession number)
~500 bp Bfa I: 158/277/61   Agrostis capillaris (AY177347b)
  Bfa I: 439/61   Agrostis vinealis (AY177332)
>500 bp Nla IV/Dde I: 57/53/187/317-329   Poa pratensis/trivialisa(AY177349b/AY177338)
  Nla IV/Dde I: 57/53/200-204/298-300 Xmn I: 197/45/367-370 Festuca rubra/ovinaa (AY177345b/AY177342)
   Xmn I: 197/45/178/154 Festuca juncifolia (AY177344)
  Nla IV/Dde I: 57/53/505-536 Hsp 92II: 125/435/86 Deschampsia flexuosa (AY177336)
   Hsp 92II: 125/405/86 Deschampsia caespitosa (AY177335)
   Hsp 92II: 336/200/86 Nardus stricta (AY177337)
   Hsp 92II: 563/86 Holcus lanatus (AY177348)
   Hsp 92II: 343/281 Luzula campestris (AY177333)
   Hsp 92II: 528/86 Phleum pratense (AY177339)
  1. a can be separated by ITS PCR-RFLP with Hin fI (Pp:335/293/76/17, Pt:293/207/127/76/17; Fr:337/297/76/17, Fo:293/230/107/76/17) b sequences from reference [6].