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Figure 3 | BMC Ecology

Figure 3

From: A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper

Figure 3

Sensitivity of detection of Blepharisma japonicum on Whatman FTA paper Sensitivity of detection of Blepharisma japonicum which has been immoblised on Whatman FTA paper. Lanes denoted M contain molecular size markers – figures refer to sizes in base pairs. Amplified products from a range of areas of FTA paper were loaded on the gel. These areas contained the following equivalent number of Blepharisma cells: 5 cells (lane 3), 2.5 cells (lane 4), 1.25 cells (lane 5), 0.625 cells (lane 6). Lane 1 contained pure Blepharisma DNA, immobilised on FTA paper, as a positive control for the PCR reaction while Lane 2 contained Paramecium caudatum DNA, on FTA paper, as a negative control. Larger areas of FTA paper (eg lanes 3 an 4) consistently inhibited the PCR reaction.

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