Figure 3From: A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paperSensitivity of detection of Blepharisma japonicum on Whatman FTA paper Sensitivity of detection of Blepharisma japonicum which has been immoblised on Whatman FTA paper. Lanes denoted M contain molecular size markers – figures refer to sizes in base pairs. Amplified products from a range of areas of FTA paper were loaded on the gel. These areas contained the following equivalent number of Blepharisma cells: 5 cells (lane 3), 2.5 cells (lane 4), 1.25 cells (lane 5), 0.625 cells (lane 6). Lane 1 contained pure Blepharisma DNA, immobilised on FTA paper, as a positive control for the PCR reaction while Lane 2 contained Paramecium caudatum DNA, on FTA paper, as a negative control. Larger areas of FTA paper (eg lanes 3 an 4) consistently inhibited the PCR reaction.Back to article page