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Table 2 Key for the Churchill, Manitoba, area Limnephilidae larvae

From: DNA barcoding facilitates associations and diagnoses for Trichoptera larvae of the Churchill (Manitoba, Canada) area

1) all gill clusters with a single filament Phanocelia canadensis
  at least some gills with clusters of 3 or more filaments (Figure 15A) 2
2) at least some gill clusters with more than 3 filaments (Figure 15A) 3
  no gill clusters with more than 3 filaments (Figure 15D) 5
3) setae present on metonotal membrane between sa2 sclerites (Figure 15B) Hesperophylax designatus
  metanotal membrane setae absent 4
4) 2nd and 3rd mesofemur with only 2 major ventral setae (Figure 15C) Lenarchus fautini
  2nd and 3rd mesofemur with numerous major ventral setae (Figure 15F) Onocosmoecus unicolor
5) setae present on metonotal membrane between sa2 sclerites (Figure 15B) Asynarchus rossi
  metanotal membrane setae absent 6
6) dorsomesal chloride epithelia present (Figure 15D) 7
  dorsomesal chloride epithelia absent 12
7) mesofemur with accessory setae on at least one lateral surface (Figure 15C) 8
  mesofemur lacking lateral accessary setae 11
8) anterolateral corner of pronotum with scurf of small, stout spicules (Figure 15E) Anabolia bimaculata
  stout spicule scurf absent 9
9) basal trochantal segment with accessory setae (Figure 16A & B) 10
  basal trochantal segment without accessory setae Asynarchus mutatus
10) ventrolateral abdominal gill series extends to 4th segment Asynarchus lapponicus
  ventrolateral abdominal gills extend to 6-7th segment Asynarchus montanus
11) ventral apotome short, about half the length of ventral ecdysial suture (Figure 16C); metanotal sa1 and sa2 sclerites small or absent Arctopora pulchella
  ventral apotome long, nearly reaching posterior margin (Figure 16D); metanotal sclerites large, obvious (Figure16E) Grammotaulius interrogationis
12) accessory setae present on at least one mesofemur lateral surface (Figure 15C &16B) 13
  mesofemur lateral accessary setae absent 19
13) basal segment of mesotrochanter with accessary setae (Figure 16B) Philarctus bergrothi
  mesotrochanter basal segment without accessory setae 14
14) dorsum of head base color pale with a distinct U-shaped dark band (Figure 10C) Nemotaulius hostilus
  base color of head dark (Figure 9I) 15
15) anterior of pronotum with transverse, wide dark band (Figure 16E) 16
  anterior of pronotom without wide, transverse dark band 17
16) head with three distinct dark bands dorsally against pale background (Figure 9G) Limnephilus infernalis
  head with dark areas and scattered darker muscle scars, without pale background laterally (Figure 9I) Limnephilus nigriceps
17) pale blotches of frontoclypeal area all located within frontoclypeal sutures (Figure 8K) Limnephilus alaicus
  at least parts of frontoclypeal pale areas located laterad of frontoclypeal sutures (Figure 8J) 18
18) the two anterior frontoclypeal pale areas are round (Figure 8J) Limnephilus ademus
  the two anterior frontoclypeal pale areas are linear and extend further forward along frontoclypeal suture (Figure 9H) Limnephilus major
19) head with dark background color with darker muscle scars. Any pale areas are small and limited to the frontoclypeal area (Figure 9L) 20
  head with large pale areas not limited to frontoclypeal area (Figure 9A) 23
20) head monochromatic brown with little evidence of pale areas in frontoclypeal area (Figure 8L) Limnephilus argenteus
  head with pale areas in frontoclypeal area (Figure 10B) 21
21) anterior ventral apotome about half the length of the ecdysial suture (Figure 16C) Limnephilus picturatus
  anterior ventral apotome nearly as long as the ecdysial suture (Figure 16D) 22
22) frontoclypeal pale blotches extend anteriorly beyond eye (Figure 9K) Limnephilus perpusillus
  frontoclypeal pale blotches do not extend anteriorly beyond eye (Figure 10B) Limnephilus sericeus
23) frontoclypeus without distinct linear dark stripe (Figure 9A) Limnephilus canadensis
  frontoclypeal stripe distinct (Figure 9D) 24
24) medial frontoclypeal band expanded anteriorly with dark coloration reaching anterior frontoclypeal corners (Figure 9C) 25
  medial frontoclypeal band not strongly expanded and without distinct dark coloration in anterior frontoclypeal corners (Figure 9F) 27
25) lateral dark bands of head reach posterior margin of head (Figure 9D) Limnephilus femoralis
  lateral dark bands curved mesad and not reaching posterior margin of head (Figure 9B) 26
26) mesonotal sa1 with numerous setae Limnephilus externus
  mesonotal sa1 with only a single seta (Figure 16F) Limnephilus extractus
27) mesonotal base color dark (Figure 17A) Limnephilus fischeri
  mesonotal base color pale (Figure 17B) 28
28) dark muscle scars broadly scattered across postgenal area (Figure 17C) Limnephilus sansoni
  postgenal muscle scars coalesced into a band 29
29) postgenal band distinct (Figure 17D) Limnephilus partitus
  postgenal band indistinct (Figure 17E) Limnephilus hageni