Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding

Table 2 Summary of the primers used in each project and sequencing success rates

Project	Primary primers	Secondary primers	Number of specimens	Success rate (%)	Number of specimens >200 bp with <2% Ns
CHUBL	LCO1490_t1/HCO2198_t1	None	94	87%	78
SAOST	LCO1490_t1/HCO2198_t1	LCO1490_t1/MLepR1; MLepF1/HCO2198_t1^a	95	83%	79
COCSA	LCO1490_t1/HCO2198_t1	CrustDF1/CrustDR1	190	65%	124
OZFWZ^b	C_LepFolF/C_LepFolR	ZplankF1_t1/ZplankR1_t1	90	70%	63
OZFWC – Plates 1 and 2^b	ZplankF1_t1/ZplankR1_t1	C_LepFolF/C_LepFolR	70	34%	24
OZFWC – Plates 3 and 4^b	LCO1490_t1/HCO2198_t1	C_LepFolF/C_LepFolR	49	37%	18
OZFWC – Plates 5-9^b	C_LepFolF/C_LepFolR	ZplankF1_t1/ZplankR1_t1	164	68%	112
Combined			752	66.2%	498

Primer sequences and references are provided in Additional file 1: Appendix A. The primer pairs used for both PCR amplification and cycle sequencing for each individual specimen are available through BOLD.
^a This primer combination involving mini-primers uses two separate PCR reactions to attempt to amplify the full-length barcode region in two fragments.
^b These samples were run on 96-well plates including mixed microcrustaceans, and thus the sample sizes do not reflect full plates.

ISSN: 1472-6785