Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding

Table 1 Summary of the methods used and numbers of genetic clusters found in each ostracod study

Project	Strategy	Dates	# of sites	Net μm	Primary target of collection	Number of clusters separated by 2% divergence	Number of specimens barcoded	Number of clusters captured that only appeared in 1 or 2 sampling strategies
CHUBL	Time- based effort	July 7–21, 2007	9	100	Ostracoda	18	78	5
SAOST	Rapid Blitz	July 9–17, 2008	14	100	Microcrustacea	18	79	6
COCSA	Liberal re-sort	July 7–21, 2007 & July 9–17, 2008	14	100	Ostracoda (re-sort)	29	124	13
OZFWZ	Fixed- protocol	June 3-Aug 25, 2010	27 (75)	64, 100, 250	Insecta (with mixed invertebrates retained)	17	63	5
OZFWC	Flexible- protocol	July 22-Aug 2, 2011	27 (42)	153	Microcrustacea	29	154	13

Study codes “CHUBL”, “SAOST”, “COCSA”, “OZFWC”, and “OZFWZ” refer to codes for each specimen on BOLD (“Process IDs”), each associated with one strategy. These five study codes can be found in three projects (COCSA, OZFWC and OZFWZ) on the Barcode of Life Database (BOLD Systems: http://www.boldsystems.org). For the column “# of sites”, all values shown without brackets represent the number of sites sampled where ostracods were found. Bracketed values represent the total number of sites for the strategy, including those where ostracods were not found. For the first three strategies, the total number of sites and number of sites with ostracods are the same due to targeted selection of suitable habitats.