Skip to main content

Table 2 PCR primer sets used for amplification of three fragments in COI DNA barcode region

From: Assessing biodiversity of a freshwater benthic macroinvertebrate community through non-destructive environmental barcoding of DNA from preservative ethanol

Primer set Sequence (5-3) Amplicon size (bp)
AD Forward: GGIGGITTTGGIAATTGAYTIGTICC 191
Reverse: CCTARIATIGAIGARAYICCIGC
BE Forward: CCIGAYATRGCITTYCCICG 224
Reverse: GTRATIGCICCIGCIARIAC
CF Forward: GITGAACIGTITAYCCICC 197
Reverse: CCIGCIGGRTCIAARAAIGAIGT