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Table 1 Percentage detection of taxa in environmental barcoding analysis of two sources of DNA using three PCR primer sets

From: Assessing biodiversity of a freshwater benthic macroinvertebrate community through non-destructive environmental barcoding of DNA from preservative ethanol

Primer set

AD

BE

CF

AD + BE

AD + CF

BE + CF

ALL

ETHANOL-DNA

69.57

78.26

67.39

84.78

82.61

86.96

86.90

TISSUE-DNA

78.26

80.43

67.39

86.96

86.96

86.96

89.10

ETHANOL + TISSUE

78.26

82.61

76.09

89.13

89.13

91.30

91.30

ETHANOL >1%

91.30

95.65

78.26

100

100

100

100

TISSUE >1%

95.65

95.65

73.91

100

100

95.65

100

  1. >1% denotes taxa with more than 1% individuals in the benthic mixture tested here.
  2. Percentages are calculated based on a total of 148 OTUs obtained in Sanger-based DNA barcode library.
  3. ETHANOL = ethanol-based DNA; TISSUE = extracted tissue-DNA.
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BMC Ecology

ISSN: 1472-6785

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