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Table 1 Percentage detection of taxa in environmental barcoding analysis of two sources of DNA using three PCR primer sets

From: Assessing biodiversity of a freshwater benthic macroinvertebrate community through non-destructive environmental barcoding of DNA from preservative ethanol

Primer set AD BE CF AD + BE AD + CF BE + CF ALL
ETHANOL-DNA 69.57 78.26 67.39 84.78 82.61 86.96 86.90
TISSUE-DNA 78.26 80.43 67.39 86.96 86.96 86.96 89.10
ETHANOL + TISSUE 78.26 82.61 76.09 89.13 89.13 91.30 91.30
ETHANOL >1% 91.30 95.65 78.26 100 100 100 100
TISSUE >1% 95.65 95.65 73.91 100 100 95.65 100
  1. >1% denotes taxa with more than 1% individuals in the benthic mixture tested here.
  2. Percentages are calculated based on a total of 148 OTUs obtained in Sanger-based DNA barcode library.
  3. ETHANOL = ethanol-based DNA; TISSUE = extracted tissue-DNA.