Table 4 List of selective amplification primers and fluorescent labels used in nine different combinations and the number of scorable and polymorphic bands generated by each combination.
From: Genetic structure of Leptopilina boulardi populations from different climatic zones of Iran
Combination | Primers and labels | Scored band | polymorphic band |
|---|---|---|---|
A | EcoRI+ACA (labeled Fam), MseI+CA | 117 | 22 (18.8%) |
B | EcoRI+AGG (labeled Joe), MseI+CA | 63 | 20 (31.74%) |
C | EcoRI+AAC (labeled Ned), MseI+CA | 65 | 12 (18346%) |
D | EcoRI+ACA (labeled Fam), MseI+CT | 101 | 15 (14.85%) |
E | EcoRI+AGG (labeled Joe), MseI+CT | 65 | 25 (38.46%) |
F | EcoRI+AAC (labeled Ned), MseI+CT | 58 | 11 (18.96) |
G | EcoRI+ACA (labeled Fam), MseI+CG | 80 | 14 (17.5%) |
H | EcoRI+AGG (labeled Joe), MseI+CG | 69 | 16 (23.19%) |
I | EcoRI+AGG (labeled Joe), MseI+CC | 52 | 12 (23.08%) |
Total band | 670 | 147 | |