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Table 4 Gene descriptions, primer sequences, efficiency, and R2 for each gene quantified with RT-qPCR.

From: Ecological transcriptomics of lake-type and riverine sockeye salmon (Oncorhynchus nerka)

Description Primers 5'-3' (forward, reverse) PCR efficiency, % R2
72 kDa type IV collagenase 5' TTC GCT GGA GAC AAG TTC TG
5' TTT GAC GAT CTT CAG GCT ACT G
80.1 0.99
Troponin 1 Slow 5' CAG GAC TTA GGA GGG AAG TTT AAG
5' AGA CAT GGC CTC CAC ATT CTT AC
84.0 0.99
Single Mit Prec 5' AGA TGT CAG CCA GAA GAC GAC
5' TCG AAT GTT GTC GCT TAA GAA TAC
105.3 0.99
40S Ribosome 5' CGA GAA GTG GTT CTA CAC CAG AG
5' GGT TCT TCT CCA CCA TCT TGA G
95.1 0.99
Malate dehydogenase 5' ATT TCT ACA GTG CAG AGA GG
5' GAA CAG GGA ATG AGT AGA TGA GG
106.5 0.99
5-amino-levuleninic acid synthase 5' ACA TCA TCC CTG TCA GAG TGT C
5' TGA TTG GGA CTT GAG AGG TAG AC
95.6 0.97