Table 4 Gene descriptions, primer sequences, efficiency, and R2 for each gene quantified with RT-qPCR.
From: Ecological transcriptomics of lake-type and riverine sockeye salmon (Oncorhynchus nerka)
Description | Primers 5'-3' (forward, reverse) | PCR efficiency, % | R2 |
|---|---|---|---|
72 kDa type IV collagenase | 5' TTC GCT GGA GAC AAG TTC TG 5' TTT GAC GAT CTT CAG GCT ACT G | 80.1 | 0.99 |
Troponin 1 Slow | 5' CAG GAC TTA GGA GGG AAG TTT AAG 5' AGA CAT GGC CTC CAC ATT CTT AC | 84.0 | 0.99 |
Single Mit Prec | 5' AGA TGT CAG CCA GAA GAC GAC 5' TCG AAT GTT GTC GCT TAA GAA TAC | 105.3 | 0.99 |
40S Ribosome | 5' CGA GAA GTG GTT CTA CAC CAG AG 5' GGT TCT TCT CCA CCA TCT TGA G | 95.1 | 0.99 |
Malate dehydogenase | 5' ATT TCT ACA GTG CAG AGA GG 5' GAA CAG GGA ATG AGT AGA TGA GG | 106.5 | 0.99 |
5-amino-levuleninic acid synthase | 5' ACA TCA TCC CTG TCA GAG TGT C 5' TGA TTG GGA CTT GAG AGG TAG AC | 95.6 | 0.97 |