Salivary Gland Gene Expression from P. papatasi collected at Swaymeh. Expression profiles were assessed as fold changes (Y axis) over the control non-sugar fed, and colony-maintained P. papatasi using the 2-ΔΔCT method. Expression profiles of P. papatasi collected in June (early in the season) and August (middle) 2007, and September (late) 2006 are displayed for SP12 (A), SP14 (B), SP28 (C), SP29 (D), SP30 (E), SP32 (F), SP36 (G), SP42 (H), and SP44 (I). Horizontal bars represent the expression mean values between the samples and each sample represents an individual fly. Asterisk (*) indicates statistically significant differences (p < 0.0006) between every two groups analyzed. Triangle, circle and square represent expression levels of sand flies collected early, in the middle and late in the season, respectively.