WNV titers in Cx. pipiens mosquitoes at the time of death. (a) Data points represent individual mosquitoes and the line represents the best-fit relationship between WNV body titer and time of death. (b) Relationship between WNV body titer and WNV leg titer in individual mosquitoes. The dotted line refers to the threshold body titer required for WNV dissemination (4.2 log10 pfu) and the linear regression analysis was completed for all values above that threshold.