Prevalence of the pathogenic chytrid fungus, Batrachochytrium dendrobatidis, in an endangered population of northern leopard frogs, Rana pipiens

Table 1 PCR results for R. pipiens individuals that were sampled with multiple tissue sampling methods on the same date.

	Tissue samples that test positive for Bd
Method	br/sw/tc	br/sw	br/tc	sw/tc	br	sw	tc	none	Total
BR, SW & TC	1	0	2	0	0	0	0	6	9
BR & SW	***	2	***	***	2	0	***	6	10
BR & TC	***	***	0	***	1	***	0	1	2
SW & TC	***	***	***	4	***	0	14	33	51
Total	1	2	2	4	3	0	14	46	72

The outcomes of the PCR assay for the 72 individuals that were tested with multiple tissue-sampling methods on the same date. The three different tissue sampling methods are bag rinse (BR), swab (SW), and toe clip (TC). The combination of methods used to sample an individual is shown in the far-left column whereas the possible outcomes of the PCR assay are shown in the top-row. For example, of the 9 individuals that were sampled with all three methods (row BR, SW & TC), 1 individual tested positive for all 3 samples (column br/sw/tc), 2 individuals tested positive for both the bag rinse and toe clip (column br/tc), and 6 animals tested negative for all 3 methods (column 'none'). The triple asterisks (***) denote PCR outcomes that are not possible for that combination of tissue sampling methods.

ISSN: 1472-6785