Predator cues reduce intraspecific trait variability in a marine dinoflagellate
© The Author(s) 2017
Received: 13 August 2016
Accepted: 15 February 2017
Published: 27 February 2017
Phenotypic plasticity is commonplace and enables an organism to respond to variations in the environment. Plastic responses often modify a suite of traits and can be triggered by both abiotic and biotic changes. Here we analysed the plastic response towards a grazer of two genotypes of the marine dinoflagellate Alexandrium fundyense, evaluated the similarity of this response and discuss potential strain-specific trade-offs. We compared the expression of the known inducible defensive traits paralytic shellfish toxin content, and chain length. The effectiveness of the induced defense was assessed by monitoring grazing rates in both strains.
Our results show that the grazer cues diminish phenotypic variability in a population by driving the phenotype towards a common defended morphotype. We further showed that the expression of the sxtA gene that initiates the paralytic shellfish toxin biosynthesis pathway does not correlate with an observed increase in the paralytic shellfish toxin analogue saxitoxin, and that toxin induction differs in its physiological characteristics in both strains.
Induced defense response in Alexandrium thus can directly affect further species interactions by reducing phenotypic variation and can result in genotype-dependent ecological trade-offs.
KeywordsAlexandrium Saxitoxin gene expression Grazer induced defense Intraspecific trait variation Predator–prey interaction
Organisms can change their traits and thus their phenotype in response to variations in the environment. However, the ability of various genotypes to produce a common, environmentally cued phenotype may differ in terms of trade-offs associated with its expression caused by genetic variation between individuals . In addition, the induction of one phenotype may indirectly influence the expression of linked traits and expose those traits to selection .
Phytoplankton cell size is a master trait that impacts growth, metabolism, and access to resources and therefore shapes the ecological niches of phytoplankton . Cell size and related morphological traits like shape, and coloniality are extremely plastic traits that depend on many environmental variables such as light levels and nutrient concentrations . Response to grazing pressure and related grazer resistance is significantly correlated with morphological traits as most grazers are confined to limited ranges of prey sizes . The grazer’s ability to detect, capture, and handle the prey sets the lower limit of size whereas morphological e.g. constrains on handling larger prey set the upper limit . For example, colony forming and size plasticity to escape grazing pressure is common in both marine (e.g. Phaeocystis globosa) and freshwater (e.g. Scenedesmus) phytoplankton . The marine diatom Skeletonema marinoi adjust its chain length in the presence of copepod grazers towards smaller chain sizes but chain length was maintained in cultures exposed to microzooplankton grazers . The bacterium Flectobacillus sp. induces filament formation when grazed by the microflagellates as single suspended cells were highly vulnerable to grazing, whereas filamentous cells were resistant to grazing .
Here we analysed the plastic response of two genotypes of the marine dinoflagellate Alexandrium fundyense in response to grazers or grazer cues. The genotypes were different in basal level of paralytic shellfish toxin production, amount of cells in chains, and ability to produce lytic extracellular substances, traits that are considered defensive. Alexandrium spp. are marine dinoflagellates and constitute together with diatoms and haptophytes the dominant groups of marine phytoplankton that accounts for approximately half of the global annual net primary production . More than 80% of the marine phytoplankton production is consumed by herbivores, thus grazing is the most important loss factor for phytoplankton [9–11]. Grazing consequently exerts a strong selective pressure on phytoplankton and structures phytoplankton communities . Alexandrium combines colony size plasticity and reduced swimming speed to avoid encounters with copepod grazers . In addition, Alexandrium produces paralytic shellfish toxins (PSTs) in response to chemical cues from certain copepods [14–19]. Genotypes within a single natural Alexandrium population showed phenotypic differences concerning the production of PSTs and further uncharacterized allelochemical compounds [20, 21]. Such an intraspecific trait variation can alter predator–prey dynamics and can lead to altered gene frequencies within a population as a result of strong grazing pressure. Induced defense as response towards grazing yet can maintain genotypic diversity, however it may also bear different trade-offs associated with the inducible defense response for each genotype [1, 22].
Our aim was to investigate genotype dependent plastic responses in Alexandrium in order to characterize the effect of grazer cues on trait variability. The two genotypes investigated here differed under standard culture condition in the amount of PSTs produced, the number of cells in colonies, the ability to produce lytic compounds and showed genetic variation in terms of gene expression patterns . We monitored the known inducible traits toxin content and chain length and investigated grazing rates of the copepods. The enhanced PSP-toxin production as response towards copepods and their cues seems to be an induction rather than an accumulations as e.g. caused by phosphorous limitation, We therefore analysed a potential direct correlation between the expression of the sxtA gene that initiates the saxitoxin biosynthesis pathway in Alexandrium and enhanced toxin production.
Alexandrium fundyense strains and zooplankton collection
The two clonal strains of A. fundyense Balech 1985 emended Anderson ; (before named as Alexandrium tamarense) were isolated from the North Sea coast east off Scotland  and grown in K-medium  in a temperature and light controlled room (salinity ~33, 18 °C, 14 h:10 h light–dark cycles, ~150 fmol m−2 s−1). The two strains, hereafter named Alex2 and Alex5 (both belong to the same population of the North American clade/ribotype group 1) are both producers of PSTs. Alex2 is further characterized by the presence of allelochemically active, unknown lytic compound(s)  whereas strain Alex5 lacks this ability.
Female Centropages typicus copepods were collected with vertical work package 2 (WP2) net hauls (200 µm mesh size) from ~20 m depth to the surface in the Gullmars fjord on the Swedish west coast. Only adult females were used in the experiments to minimize the variability between treatments. The copepods were maintained in the laboratory in filtered seawater (0.2 µm; salinity ~33) and fed Rhodomonas baltica (from the University of Gothenburg Marine Culture Collection, GUMACC) until the start of the experiments.
Direct grazing experiments
For both A. fundyense strains 18 bottles with 500 mL (~5 × 106 cells L−1) cultures in K/10 medium  was prepared. Half of the bottles received 10 C. typicus females per bottle, and the remaining bottles were kept as copepod free controls. All bottles were incubated in a temperature and light controlled room (salinity ~33, 18 °C, 14 h:10 h light–dark cycles, ~150 fmol m−2 s−1). Three replicates of the grazed and control treatments were harvested after 12, 48, and 72 h (see “sampling procedure” section below). Clearance rates were calculated using the equations of Frost .
The experiments with fed or starved copepods kept in cages were conducted in 500 mL glass bottles with cages made out of 50 mL polypropylene tubes with a 10 µm plankton mesh at the bottom and incubation conditions as described above. The plankton mesh constrained the organisms to their compartment (flask or cage) while allowing waterborne-cues to move between the compartments. For each A. fundyense strain, 9 bottles were set up consisting of three replicates of copepod-free controls, fed C. typicus, and starved C. typicus. Each bottle received 450 mL of A. fundyense culture with ~4 × 106 cells L−1 in K/10 medium . Cages were deployed into the flasks and filled with 30 mL of the same culture for the control treatments and the fed treatments. The flasks for the starved treatment received 30 mL of K/10 medium instead. Each cage of the fed and starved treatments received 10 C. typicus individuals i.e. the same amount as in the direct grazing experiment. The cages were gently moved up and down 5 times a day in order to promote the exchange of chemical cues between the compartments. The experiments were terminated after 48 h.
Bottles were sealed and carefully turned over 7 times to ensure equal mixing of the culture. The direct grazing experiment samples were pre-filtered through a submerged 64 µm nylon mesh to remove copepods and copepods eggs. 60–70 mL were sub-sampled for enumeration and sizing of cells and chains using a coulter counter (Micromeritics, Norcross, USA) mounted with a 100 µm orifice tube and continuous stirring. A known volume of the subsamples was then suction filtered onto glass-fibre filters (Whatman) and stored at −20 °C until toxin extraction and analysis.
The remaining culture was concentrated on a 10 µm nylon mesh to collect the A. fundyense cells. The 10 µm mesh was rinsed into a 50 mL centrifuge tube with sterile filtered seawater and immediately centrifuged at 4 °C for 5 min for RNA samples. The supernatant was discharged and the pellet immediately mixed with 1 mL 60 °C hot TriReagent (Sigma-Aldrich, Steinheim, Germany) and transferred to a cryovial with acid washed glass beads. The cryovial was vortexed for 10 s and submerged into liquid nitrogen. The samples were stored at −80 °C until RNA isolation.
Paralytic shellfish toxins samples (PST) were freeze-dried and extracted in 1 mL 0.05 M acetic acid (aq) through three consecutive freeze–thaw cycles. Extracts were filtered (GF/F) and stored frozen in glass vials. Samples were hydrolyzed with 0.1 M HCl at 100 °C for 10 min to transform C-toxins into their corresponding carbamates. We used a modified version of the method described by Asp and co-workers  to analyze all carbamate PSTs in a single run . HPLC analysis was carried out on a Hitachi-7000 system equipped with a Genesis C8 column, (Vymac, 4 μm, 150*3 mm). A gradient between elution with 2 mM L−1 sodiumheptanesulfonate in 10 mM L−1 ammonium-phosphate buffer (pH 7.1) and 2 mM L−1 sodiumheptanesulfonate in 30 mM L−1 ammonium phosphate buffer (pH 7.1): acetonitrile (96:4) was used to separate PSTs. After the separation, toxins were oxidized with 7 mM periodic acid in 50 mM sodium phosphate buffer (pH 9.0) in a PEEK capillary (10 m, 80 °C). The oxidation was terminated with 0.5 M acetic acid before fluorescent detection at λex = 330 nm, λem = 390 nm. Toxin standards were obtained from the certified reference materials program, National Research Council, Halifax, Canada.
Total RNA isolation
TriReagent fixed and frozen cells were lysed using a Bio101 FastPrep instrument (Thermo Savant Illkirch, France) at maximum speed (6.5 m s−1) for 2 × 45 s. Lysed cells were cooled on ice, 200 µL chloroform was added and vortexed for 20 s. The samples were transferred to a phase lock tube after 5 min incubation at room temperature (Eppendorf, Hamburg; Germany) and incubated for another 5 min followed by centrifugation for 15 min at 13,000×g and 4 °C. The upper aqueous phase was transferred to a new tube and mixed with the same volume isopropanol, 1/10 volume of 3 M Na-acetate (pH 5.5; Ambion by Life Technologies, Carlsbad, California, USA) and 2 µL linear polyacrylamide (Ambion). Total RNA was precipitated for 90 min at −20 °C and collected by centrifugation for 20 min and 13,000×g at 4 °C. The obtained pellet was washed twice, first with 1 mL 70% EtOH followed by 1 mL EtOH absolute; the RNA pellet was dried for 1 min at 37 °C and resolved in 30 µL RNase free water (Qiagen, Hilden). RNA quality check was performed using a NanoDrop ND-1000 spectrometer (PeqLab, Erlangen, Germany) for purity and the RNA Nano Chip Assay with the 2100 Bioanalyzer device (Agilent Technologies, Santa Clara, California, USA) was just to examine the integrity of the extracted RNA. Potential DNA contaminations of RNA samples were tested by amplification of the 28 s rDNA gene. We considered our samples to be free of DNA as the respective gene could be amplified in positive controls only (containing both, RNA and added DNA).
Expression analysis of the putative sxtA gene via RT-qPCR
The quantitative expression of the putative sxtA gene in Alex2 was evaluated via RT-qPCR using the method described in Freitag et al. . For this RT-qPCR analysis, we only considered treatments were a significant increase in saxitoxin on the total PST profile compared to control cultures was observed (Alex2, direct grazing experiment, 48 and 72 h). Prior to cDNA synthesis, 500 ng of total RNA of the samples of interest were spiked with 10 ng of MA mRNA and 1 ng of NSP mRNA that served as an external Ref. . The cDNA was synthesized with the Omniscript RT kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions with anchored oligo (dT) 20 primers. The synthesized cDNAs were diluted 1:5 and 2 µL were used to analyze the expression of the putative sxtA gene, the MA gene and the NSP gene with a SYBRgreen assay according to the manufactures protocol (Applied Biosystems/Life Technologies, Carlsbad, California, USA). Primers for the putative sxtA gene fragment had the sequences: 5′GCGAGACCGACGAGAAGTTC′3 and 5′AGCCGCTTGCGCTGAAG′3; primer sequences for the MA and NSP gene are given by Freitag et al. . The qPCR reaction were carried out in a StepOnePlus™ Real-Time PCR System-device (Applied Biosystem by Life Technologies, Carlsbad, California, USA) with the following cycling parameters: 10 min initial denaturation at 95 °C, 40 cycles of 95 °C for 15 s and 60 °C for 1 min. A melting curve analysis was performed at the end of the reactions to verify the formation of a single PCR product in each reaction. In addition, standard curves with 6 points and a serial dilution factor of 1:10 starting with 10 ng PCR product from each gene were also analyzed with the SYBRgreen assay using the same primers and parameters. PCR products for the standard curves for the sxtA gene were obtained from the prepared cDNAs and the following primers: 5′CCGCCATATGTGCTTGTTTG′3 and 5′AGCTCCCTGTACACCTCTGC′3. Expression ratios were calculated using the equations of Pfaffl .
Alex5 was generally less prone to form chains (Fig. 1C, D). Yet, exposure to both direct grazing and grazer cues resulted in a significantly reduced average size (mean diameter) also for this strain (~4% after 48 h and ~6% after 72 h, two-way ANOVA p < 0.05, TukeyHSD p < 0.05) (Additional file 1).
Different sampling times led to observable variations of cell size distributions in both strains (Fig. 1). Cells sampled at the time point 12 h have been collected just before the onset of the dark cycle that triggers cell division, and therefore also contain several cells that have accumulated enough biovolume to divide. Cells sampled at time points 48 and 72 h yet have been sampled after the dark cycle in the morning after cell division, thus showing a less scattered distribution with smaller overall cell sizes.
In the treatment with waterborne cues from caged fed and caged starved copepods, the cell size means significantly decreased by ~6.5% (one-way ANOVA p < 0.05, TukeyHSD p < 0.05) (Fig. 1D; Additonal file 1) showing that the response does not require any physical contact between copepods and algal cells.
PST-contents and clearance rates
The treatment with waterborne-cues from caged fed and starved copepods however showed significant differences in the mean PST content for Alex2 only (one-way ANOVA p < 0.05, Fig. 2B), where PST content increased compared to the control (TukeyHSD p < 0.05).
Clearance rates on Alex2 significantly decreased from 1.7 mL per female per hour to 0.4 mL per female per hour (one-way ANOVA p < 0.05, Fig. 2A) during the direct grazing experiment. Clearance of copepods grazing on Alex5 cultures, however, remained constant around 0.4 mL per female per hour (Fig. 2C).
PST profiles and differential expression of the sxtA gene
The phenotypic differences show that Alex5 expresses the ‘defended’ phenotype, with smaller units (cells and/or cell chains) with higher toxin content already before exposure to grazers or grazer cues (Figs. 1, 2). Contrarily, Alex2 exhibited a less defended phenotype, with more cells in chains and lower toxin content in the absence of grazers or grazer cues. This was also accompanied by significant differences in the clearance rate of the copepods on the two genotypes. The clearance rates during the initial 12 h were fourfold lower for Alex5 compared to Alex2 (Fig. 2A, C). However, the grazing rates on Alex2 decrease to the same level after the induction of the defended phenotype (48 h, Figs. 1, 2). The phenotype expressed by Alex2 prior to defense induction is less vulnerable to unicellular heterotrophic grazers  and the allelochemicals produced by this strain did not seem to protect them against grazing copepods. Hence, the consequence of grazer exposure for Alex2 is a phenotypic shift towards less vulnerability towards copepod grazing. The splitting of cell chains in Alex2 may cause ecological costs under natural conditions i.e. a reduction of the ability to perform vertical migrations to retrieve nutrients at depth  and to resist unicellular grazers and competitors due to a reduced amount of cells in chains accompanied by a decreased concentration of allelochemicals surrounding the cells of this strain [30, 31]. These ecological costs may not only be strain-specific as they can indirectly influence the survival of conspecifics protected by the allelochemical cloud of this strain [23, 32]. Yet, the ability of A. fundyense to induce defense at different levels (morphological and physiological, as well as behavioural (see also Selander et al. ), offers flexibility in the applied defense strategy and allows to lower the predation risk at feasible costs depending on trade-offs that are themselves dependent on the environment and genotype [33, 34].
The increase in toxin biosynthesis differs in its physiological characteristics between the two strains: Alex5 increased the amount of PSTs without changing the relative composition of individual PSTs; in Alex2 the PST profile changed to a higher proportion of the saxitoxin derivate (Fig. 3). The higher amount of PST and in particular saxitoxin in Alex2 did, however, at the measured time points not correlate with a higher expression of the putative sxtA gene  that initiate the saxitoxin biosynthesis pathway as verified via RT-qPCR (Fig. 4). The sxtA gene product might not be the limiting step in the biosynthesis of saxitoxin or might have been upregulated already at the onset of the induced defense response prior to the observed morphological and physiological changes. Further, the biosynthesis of saxitoxin could be regulated by other means e.g. post-transcriptional, by the regulation of other enzymes participating in this pathway  or by the provision of precursors and reduction equivalents. The observed differences in the two strains may also point towards a convoluted biosynthesis pathway where several routes could lead to an increase in intracellular PST content. An increase in PSTs could therefore be as well associated with changes in other traits where beneficial trait associations led to a selective advantage. For example, we cannot rule out that the PST increase per biovolume is purely due to a decrease of cell size in our experiment. However, previous studies with the same genotype Alex5 showed increased PST contents after 48 h exposure to copepods on a per cell basis, irrespective of the biolvolume . Selander et al. showed that after removal of copepods, grazer-induced PST contents in A. fundyense returned to control levels after approximately 5 cell divisions, whereas the average biovolume returned to control levels after one to two cell divisions. Thus A. fundyense cells remained more toxic compared to controls with comparable biovolumes .
With the cage experiment design we demonstrated that the copepod cues alone are sufficient to induce the expression of defensive features. However, due to a potential signal dampening effect the signals alone lead to a weaker response compared to the direct grazing experiments (Figs. 1, 2) [17, 30, 37, 38]. The reduction of cell chains, however, remained at comparable levels to the direct grazing experiment in both strains (Figs. 1, 2). A meta-analysis on herbivore resistance in plants presumed that traits other than secondary metabolites (e.g. morphology, life history, phenology, primary chemistry and physiology) are more effective in defense . As the effect of increased toxin content in Alexandrium on grazing rates strongly depends on the grazer abundance, species composition and local adaptations [37, 40–45], the significance of increased toxin content for defense is still unclear, particularly because intoxication of the grazer benefits the whole population and additionally potential competitors, unless the more toxic cells are rejected by grazers and directly benefit from their inducible toxicity [15, 37, 41]. The observed morphological changes are preventive by reducing encounter rates with grazers  and may therefore directly contribute increased fitness to responding cells.
In conclusion, we showed that the presence of copepods has the potential to induce the formation of a comparable phenotype from initially different genotypes which highlights the selective pressure that emanates from grazers in the pelagic realm. We could further show that the morphological changes might be stronger correlated to the induced defense response than changes in PST-content are. The induced defense response will reduce the trait variability for natural selection to operate on; the costs for its induction however can be different for each strain on several levels i.e. in terms of physiological and ecological costs. Selection on different genotypes can therefore occur in the presence of plasticity due to different costs and trade-offs that arise for the respective genotype. The occurrence of multiple environmental cues to respond to in nature however might equalize the effects of strain-specific trade-offs within populations.
paralytic shellfish toxin(s)
Conceived and designed the experiments: SW ES UJ. Performed the experiments: SW ES. Analyzed the data: SW ES. Wrote the paper: SW ES UJ. All authors read and approved the final manuscript.
The authors thank Anke Stüken for her help with setting up the RT-qPCR analysis for the sxtA gene. Further, the authors thank the two anonymous reviewers for their constructive comments.
The authors declare that they have no competing interests.
Availability of data and materials
The datasets generated and/or analysed during the current study are available in the PANGAEA® Data Publisher repository: https://doi.org/10.1594/PANGAEA.871148.
Financial support was provided by the PACES research program of the Alfred-Wegener-Institute Helmholtz-Zentrum für Polar-und Meeresforschung, by the German Research Foundation (DFG) Priority Programme DynaTrait (1704; JO 702/7-1) and by FORMAS research Grant No 223-2012-693 to Erik Selander.
Role of funding: PACES: study design, data analysis and interpretation, manuscript writing; DFG: data analysis and interpretation, manuscript writing; FORMAS: study design, data analysis and interpretation, manuscript writing.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Bolnick DI, Amarasekare P, Araújo MS, Bürger R, Levine JM, Novak M, Rudolf VHW, Schreiber SJ, Urban MC, Vasseur DA. Why intraspecific trait variation matters in community ecology. Trends Ecol Evol. 2011;26(4):183–92.View ArticlePubMedPubMed CentralGoogle Scholar
- Pfennig DW, Wund MA, Snell-Rood EC, Cruickshank T, Schlichting CD, Moczek AP. Phenotypic plasticity’s impacts on diversification and speciation. Trends Ecol Evol. 2010;25(8):459–67.View ArticlePubMedGoogle Scholar
- Litchman E, Klausmeier CA. Trait-based community ecology of phytoplankton. Annu Rev Ecol Evol Syst. 2008;39(1):615–39.View ArticleGoogle Scholar
- Hansen B, Bjornsen PK, Hansen PJ. The size ratio between planktonic predators and their prey. Limnol Oceanogr. 1994;39(2):395–403.View ArticleGoogle Scholar
- Van Donk E, Ianora A, Vos M. Induced defences in marine and freshwater phytoplankton: a review. Hydrobiologia. 2011;668:3–19.View ArticleGoogle Scholar
- Bergkvist J, Thor P, Jakobsen HH, Wängberg S-Å, Selander E. Grazer-induced chain length plasticity reduces grazing risk in a marine diatom. Limnol Oceanogr. 2012;57(1):318–24.View ArticleGoogle Scholar
- Corno G, Jürgens K. Direct and indirect effects of protist predation on population size structure of a bacterial strain with high phenotypic plasticity. Appl Environ Microbiol. 2006;72(1):78–86.View ArticlePubMedPubMed CentralGoogle Scholar
- Falkowski PG, Katz ME, Knoll AH, Quigg A, Raven JA, Schofield O, Taylor FJR. The evolution of modern eukaryotic phytoplankton. Science. 2004;305:354–60.View ArticlePubMedGoogle Scholar
- Calbet A. Mesozooplankton grazing effect on primary production: a global comparative analysis in marine ecosystems. Limnol Oceanogr. 2001;46:1824–30.View ArticleGoogle Scholar
- Calbet A, Landry MR. Phytoplankton growth, microzooplankton grazing, and carbon cycling in marine systems. Limnol Oceanogr. 2004;49:51–7.View ArticleGoogle Scholar
- Cyr H, Pace ML. Allometric theory-extrapolations from individuals to communities. Ecology. 1993;74:1234–45.View ArticleGoogle Scholar
- Verschoor AM, Vos M, van der Stap I. Inducible defences prevent strong population fluctuations in bi- and tritrophic food chains. Ecol Lett. 2004;7:1143–8.View ArticleGoogle Scholar
- Selander E, Jakobsen HH, Lombard F, Kiørboe T. Grazer cues induce stealth behavior in marine dinoflagellates. Proc Natl Acad Sci. 2011;108:4030–4.View ArticlePubMedPubMed CentralGoogle Scholar
- Selander E, Kubanek J, Hamberg M, Andersson MX, Cervin G, Pavia H. Predator lipids induce paralytic shellfish toxins in bloom-forming algae. Proc Natl Acad Sci. 2015;112(20):6395–400.View ArticlePubMedPubMed CentralGoogle Scholar
- Selander E, Thor P, Toth G, Pavia H. Copepods induce paralytic shellfish toxin production in marine dinoflagellates. Proc R Soc B-Biol Sci. 2006;273:1673–80.View ArticleGoogle Scholar
- Selander E, Fagerberg T, Wohlrab S, Pavia H. Fight and flight in Dinoflagellates? Kinetics of simultaneous grazer-induced responses in Alexandrium tamarense. Limonol Oceanogr. 2012;57:58–64.View ArticleGoogle Scholar
- Wohlrab S, Iversen MH, John U. A molecular and co-evolutionary context for grazer induced toxin production in Alexandrium tamarense. PLoS ONE. 2010;5:e15039.View ArticlePubMedPubMed CentralGoogle Scholar
- Yang I, Selander E, Pavia H, John U. Grazer-induced toxin formation in dinoflagellates: a transcriptomic model study. Eur J Phycol. 2011;46:66–73.View ArticleGoogle Scholar
- Senft-Batoh CD, Dam HG, Shumway SE, Wikfors GH. A multi-phylum study of grazer-induced paralytic shellfish toxin production in the dinoflagellate Alexandrium fundyense: a new perspective on control of algal toxicity. Harmful Algae. 2015;44:20–31.View ArticleGoogle Scholar
- Tillmann U, Hansen P. Allelopathic effects of Alexandrium tamarense on other algae: evidence from mixed growth experiments. Aquat Microb Ecol. 2009;57:101–12.View ArticleGoogle Scholar
- Alpermann TJ, Tillmann U, Beszteri B, Cembella AD, John U. Phenotypic variation and genotypic diversity in a planktonic population of the toxigenic marine dinoflagellate Alexandrium tamarense (Dinophyceae). J Phycol. 2010;46:18–32.View ArticleGoogle Scholar
- Tillmann U, Alpermann TL, da Purificação RC, Krock B, Cembella A. Intra-population clonal variability in allelochemical potency of the toxigenic dinoflagellate Alexandrium tamarense. Harmful Algae. 2009;8:759–69.View ArticleGoogle Scholar
- Wohlrab S, Tillmann U, Cembella A, John U. Trait changes induced by species interactions in two phenotypically distinct strains of a marine dinoflagellate. ISME. 2016.
- John U, Litaker RW, Montresor M, Murray S, Brosnahan ML, Anderson DM. Formal revision of the Alexandrium tamarense Species Complex (Dinophyceae) taxonomy: the introduction of five species with emphasis on molecular-based (rDNA) classification. Protist. 2014;165(6):779–804.View ArticlePubMedPubMed CentralGoogle Scholar
- Keller MD, Selvin RC, Claus W, Guillard RRL. Media for the culture of oceanic ultraphytoplankton. J Phycol. 1987;23:633–8.View ArticleGoogle Scholar
- Frost BW. Effects of size and concentration of food particles on the feeding behavior of the marine planktonic copepod Calanus pacificus. Limnol Oceanogr. 1972;17:805–15.View ArticleGoogle Scholar
- Asp TN, Larsen S, Aune T. Analysis of PSP toxins in Norwegian mussels by a post-column derivatization HPLC method. Toxicon. 2004;43:319–27.View ArticlePubMedGoogle Scholar
- Freitag M, Beszteri S, Vogel H, John U. Effects of physiological shock treatments on toxicity and polyketide synthase gene expression in Prymnesium parvum (Prymnesiophyceae). Eur J Phycol. 2011;46:193–201.View ArticleGoogle Scholar
- Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001;29:e45.View ArticlePubMedPubMed CentralGoogle Scholar
- Harvell DC, Tollrian R. Why inducible defenses? In: Tollrian R, Harvell DC, editors. The ecology and evolution of inducible defenses. Princeton: Princeton University Press; 1999. p. 3–9.Google Scholar
- Jonsson PR, Pavia H, Toth G. Formation of harmful algal blooms cannot be explained by allelopathic interactions. Proc Natl Acad Sci USA. 2009;106:11177–82.View ArticlePubMedPubMed CentralGoogle Scholar
- John U, Tillmann U, Hülskötter J, Alpermann TJ, Wohlrab S, Van de Waal DB. Intraspecific facilitation by allelochemical mediated grazing protection within a toxigenic dinoflagellate population. Proc R Soc Lond B: Biol Sci. 2015;282(1798):20141268.View ArticleGoogle Scholar
- Ellner SP. Rapid evolution: from genes to communities, and back again? Funct Ecol. 2013;27(5):1087–99.View ArticleGoogle Scholar
- Bjærke O, Jonsson PR, Alam A, Selander E. Is chain length in phytoplankton regulated to evade predation? J Plankton Res. 2015;37:1110–9.Google Scholar
- Stüken A, Orr RJS, Kellmann R, Murray SA, Neilan BA, Jakobsen KS. Discovery of nuclear-encoded genes for the neurotoxin saxitoxin in dinoflagellates. PLoS ONE. 2011;6:e20096.View ArticlePubMedPubMed CentralGoogle Scholar
- Perini F, Galluzzi L, Dell’Aversano C, Iacovo E, Tartaglione L, Ricci F, Forino M, Ciminiello P, Penna A. SxtA and sxtG gene expression and toxin production in the Mediterranean Alexandrium minutum (Dinophyceae). Marine Drugs. 2014;12(10):5258.View ArticlePubMedPubMed CentralGoogle Scholar
- Senft-Batoh CD, Dam HG, Shumway SE, Wikfors GH, Schlichting CD. Influence of predator–prey evolutionary history, chemical alarm-cues, and feeding selection on induction of toxin production in a marine dinoflagellate. Limnol Oceanogr. 2015;60(1):318–28.View ArticleGoogle Scholar
- Bergkvist J, Selander E, Pavia H. Induction of toxin production in dinoflagellates: the grazer makes a difference. Oecologia. 2008;156:147–54.View ArticlePubMedGoogle Scholar
- Carmona D, Lajeunesse MJ, Johnson MTJ. Plant traits that predict resistance to herbivores. Funct Ecol. 2011;25:358–67.View ArticleGoogle Scholar
- Turner JT, Tester PA. Toxic marine phytoplankton, zooplankton grazers, and pelagic food webs. Limnol Oceanogr. 1997;42:1203–14.View ArticleGoogle Scholar
- Teegarden GJ. Copepod grazing selection and particle discrimination on the basis of PSP toxin content. Mar Ecol Prog Ser. 1999;181:163–76.View ArticleGoogle Scholar
- Turner TJ, Doucette JG, Powell LC, Kulis MD, Keafer AB, Anderson MD. Accumulation of red tide toxins in larger size fractions of zooplankton assemblages from Massachusetts Bay. USA. Mar Ecol Prog Ser. 2000;203:95–107.View ArticleGoogle Scholar
- Colin SP, Dam H. Testing for resistance of pelagic marine copepods to a toxic dinoflagellate. Evol Ecol. 2004;18:355–77.View ArticleGoogle Scholar
- Teegarden GJ, Campbell RG, Anson DT, Ouellett A, Westman BA, Durbin EG. Copepod feeding response to varying Alexandrium spp. cellular toxicity and cell concentration among natural plankton samples. Harmful Algae. 2008;7:33–44.View ArticleGoogle Scholar
- Dam HG, Haley ST. Comparative dynamics of paralytic shellfish toxins (PST) in a tolerant and susceptible population of the copepod Acartia hudsonica. Harmful Algae. 2011;10:245–53.View ArticleGoogle Scholar