Table 1 Primers and references for PCR amplification conditions of markers employed in the current study

16S rRNA

Cyanobacterial genome

Primer 1 (5' CTC TGT GTG CCT AGG TAT CC 3') and Primer 2 (5' GGG GAA TTT TCC GCA ATG GG 3')

[51]

Cyanobacteria

COI

Mitochondrial

GazF1 (5' TCA ACA AAT CAT AAA GAT ATT GG 3') and GazR1 (5' ACT TCT GGA TGT CCA AAA AAY CA 3')

[52]

Rhodophyta

ITS

Nuclear

CladoITS-9 F (5′ CCG CCC GTC GCT CCT ACC GAT TGG GTG TG 3′) and CladoITS7R (5′ TCC CTT TTC GCT CGC CGT TAC TA 3′)

[93]

Chlorophyta

LSU

Nuclear

C′1 (5′ ACC CGC TGA ATT TAA GCA TAT 3′) and D2 (5′ TCC GTG TTT CAA GAC GG 3′); C1FL (5' ACC CGC TGA ACT TAA GC 3′) and D2FL (5' GGT CCG TGT TTC AAG 3′)

[53],[54]

Chlorophyta

SSU

Nuclear

SR4 (5' AGC CGC GGT AAT TCC AGC T 3') and SR9 (5' AAC TAA GAA CGG CAT GCA C 3')

[94]

All eukaryotic lineages

rbcL

Plastid

F160 (5' CCT CAA CCA GGA GTA GAT CC 3') and rbcLR (5' ACA TTT GCT GTT GGA GTC TC 3') (Rhodophyta); Comp1 (5' GAA TCT TCT ACA GCA ACT TGG AC 3') and Comp 2 (5' GCA TCT CTT ATT ATT TGA GGA CC 3') (Rhodophyta); RH1 (5' ATG TCA CCA CAA ACA GAA ACT AAA GC 3') and rbcL-R1351-Zyg (5' AGC AGC TAA TTC AGG ACT CAA 3') (Streptophyta)

[55]-[57]

Rhodophyta and Streptophyta

tufA

Plastid

tufAF (5' TGA AAC AGA AMA WCG TCA TTA TGC 3') and tufAR (5' CCT TCN CGA ATM GCR AAW CGC 3')

[58]

Streptophyta and Chlorophyta

UPA

Plastid

p23SrV_f1 (5' GGA CAG AAA GAC CCT ATG AA 3') and p23SrV_r1 (5' TCA GCC TGT TAT CCC TAG AG 3')

[61]

All lineages