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Table 3 List of primers and adaptor used for the AFLP analysis

From: Genetic structure of Leptopilina boulardi populations from different climatic zones of Iran

Type

Name

Sequence 5'-3'

Adaptor

EcoRI adaptor/F

CTCGTAGACTGCGTACC

 

EcoRI adaptor/R

AATTGGTACGCAGTCTAC

 

MseI adaptor/F

GACGATGAGTCCTGAG

 

MseI adaptor/R

TACTCAGGACTCAT

Primer, pre select. amp.

EcoRI Preamp.

GAC TGCGTACCAATTCA*

 

MseI Preamp.

GATGAGTCCTGAGTAAC*

Primer, select. amp

EcoRI Select.

GACTGCGTACCAATTCA NN *

.

MseI Select.

GATGAGTCCTGAGTAAC N *

  1. *Nucleotides in bold are the fixed extended base in the 3' end of the primers and N are the variable extension to provide different primer combination, see above.