Figure 2
Feeding assay experimental design for snails collected for RNA isolation. Snails were randomly placed into one of nine groups, time zero group (To), a control group (C1,C2, C3), or one of seven gorgonian diet groups (A, Briareum asbestinum; B, Eunicea mammosa; C, Gorgonia ventalina; D, Pseudopterogoria acerosa; E, Pseudopterogoria americana; F, Pseudopterogoria elisabethae; G, Plexaura homomalla). Digestive glands collected after completion of this feeding assay were used for RNA isolation and mRNA expression analysis.